UniProt UniProt http://www.uniprot.org Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Biochemistry 2010 imex curation Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu Accepted 2019-JUN-05 AT 09:31 BST AT 09:31 BST by LIVIAP Biochemistry 2010 Only protein-protein interactions imex curation imex curation Sorry, I forgot to delete the remark internal when I found the evidence of purification.; " Mats and Meths state: "The regions that contained the highest homology in each sLRP were CR3-5 (831-956), CR16-18 (2712-2838), and CR25-27 (3471-3516) (numbering according to mature LRP-1). " I noticed that you shifted all the ranges according to the post-processed chain in Uniprot. Please either add a data-processing comment explaining the shifting or replace the UniprotAc of lrp1 with the post-processed id (PRO_0000017317) and maintain the same numbering as in the publication. " - neither of these have ever been common practice when a signal/transit peptide has been ignored in the numbering. We only ever use a chain when its a peptide, not just a signal peptide which has potentially been clipped off, and we've never added data processing comments for correcting residue numbering - apart from anything else, the free text comment will be wrong but cannot be automatically corrected if the the protein gets a sequence update. Otherwise all done. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Biochemistry 2010 imex curation Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu Accepted 2019-JUN-05 AT 09:31 BST AT 09:31 BST by LIVIAP Biochemistry 2010 Only protein-protein interactions imex curation imex curation Sorry, I forgot to delete the remark internal when I found the evidence of purification.; " Mats and Meths state: "The regions that contained the highest homology in each sLRP were CR3-5 (831-956), CR16-18 (2712-2838), and CR25-27 (3471-3516) (numbering according to mature LRP-1). " I noticed that you shifted all the ranges according to the post-processed chain in Uniprot. Please either add a data-processing comment explaining the shifting or replace the UniprotAc of lrp1 with the post-processed id (PRO_0000017317) and maintain the same numbering as in the publication. " - neither of these have ever been common practice when a signal/transit peptide has been ignored in the numbering. We only ever use a chain when its a peptide, not just a signal peptide which has potentially been clipped off, and we've never added data processing comments for correcting residue numbering - apart from anything else, the free text comment will be wrong but cannot be automatically corrected if the the protein gets a sequence update. Otherwise all done. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Biochemistry 2010 imex curation Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu Accepted 2019-JUN-05 AT 09:31 BST AT 09:31 BST by LIVIAP Biochemistry 2010 Only protein-protein interactions imex curation imex curation Sorry, I forgot to delete the remark internal when I found the evidence of purification.; " Mats and Meths state: "The regions that contained the highest homology in each sLRP were CR3-5 (831-956), CR16-18 (2712-2838), and CR25-27 (3471-3516) (numbering according to mature LRP-1). " I noticed that you shifted all the ranges according to the post-processed chain in Uniprot. Please either add a data-processing comment explaining the shifting or replace the UniprotAc of lrp1 with the post-processed id (PRO_0000017317) and maintain the same numbering as in the publication. " - neither of these have ever been common practice when a signal/transit peptide has been ignored in the numbering. We only ever use a chain when its a peptide, not just a signal peptide which has potentially been clipped off, and we've never added data processing comments for correcting residue numbering - apart from anything else, the free text comment will be wrong but cannot be automatically corrected if the the protein gets a sequence update. Otherwise all done. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Biochemistry 2010 imex curation Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu Accepted 2019-JUN-05 AT 09:31 BST AT 09:31 BST by LIVIAP Biochemistry 2010 Only protein-protein interactions imex curation imex curation Sorry, I forgot to delete the remark internal when I found the evidence of purification.; " Mats and Meths state: "The regions that contained the highest homology in each sLRP were CR3-5 (831-956), CR16-18 (2712-2838), and CR25-27 (3471-3516) (numbering according to mature LRP-1). " I noticed that you shifted all the ranges according to the post-processed chain in Uniprot. Please either add a data-processing comment explaining the shifting or replace the UniprotAc of lrp1 with the post-processed id (PRO_0000017317) and maintain the same numbering as in the publication. " - neither of these have ever been common practice when a signal/transit peptide has been ignored in the numbering. We only ever use a chain when its a peptide, not just a signal peptide which has potentially been clipped off, and we've never added data processing comments for correcting residue numbering - apart from anything else, the free text comment will be wrong but cannot be automatically corrected if the the protein gets a sequence update. Otherwise all done. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E. Biochemistry 2010 imex curation Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Guttman M., Prieto JH., Croy JE., Komives EA. The supp mat appears to be for the wrong paper so I've only added data which is described in the main paper. I have also not included fusion constructs. ekomives@ucsd.edu Accepted 2019-JUN-05 AT 09:31 BST AT 09:31 BST by LIVIAP Biochemistry 2010 Only protein-protein interactions imex curation imex curation Sorry, I forgot to delete the remark internal when I found the evidence of purification.; " Mats and Meths state: "The regions that contained the highest homology in each sLRP were CR3-5 (831-956), CR16-18 (2712-2838), and CR25-27 (3471-3516) (numbering according to mature LRP-1). " I noticed that you shifted all the ranges according to the post-processed chain in Uniprot. Please either add a data-processing comment explaining the shifting or replace the UniprotAc of lrp1 with the post-processed id (PRO_0000017317) and maintain the same numbering as in the publication. " - neither of these have ever been common practice when a signal/transit peptide has been ignored in the numbering. We only ever use a chain when its a peptide, not just a signal peptide which has potentially been clipped off, and we've never added data processing comments for correcting residue numbering - apart from anything else, the free text comment will be wrong but cannot be automatically corrected if the the protein gets a sequence update. Otherwise all done. dimyristoylphosphatidyl choline dimyristoylphosphatidyl choline (1)-(7-myristoyl-4-oxido-10-oxo-3,5,9-trioxa-4-phosphatricosyl)trimethylammonium 4-oxide 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine 1,2-ditetradecanoyl-sn-glycero-3-phosphocholine 2,3-bis(tetradecanoyloxy)propyl 2-(trimethylammonio)ethyl phosphate [2-({[2,3-bis(tetradecanoyloxy)propyl] phosphonato}oxy)ethyl]trimethylazanium dimyristoyl-phosphatidylcholine dimyristoylphosphatidylcholine DMPC 2,3-bis(tetradecanoyloxy)propyl 2-(trimethylazaniumyl)ethyl phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) CITHEXJVPOWHKC-UHFFFAOYSA-N CITHEXJVPOWHKC-UHFFFAOYSA-N ldlr_human Low-density lipoprotein receptor LDLR protein protein human Homo sapiens Human MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA DIP protein P01130 original sequence version: 115 DIP protein P01130 original sequence version: 122 DIP protein P01130 original sequence version: 140 DIP protein P01130 original sequence version: 173 A4C28E9B8BADAD5E amrp_human Alpha-2-macroglobulin receptor-associated protein Low density lipoprotein receptor-related protein-associated protein 1 LRPAP1 A2MRAP protein protein human Homo sapiens Human MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL DIP protein P30533 original sequence version: 105 DIP protein P30533 original sequence version: 103 D56A7DA754125CFA apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH DIP protein P02649 original sequence version: 197 DIP protein P02649 original sequence version: 146 91AFC04210A30689 calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) lrp1_human Prolow-density lipoprotein receptor-related protein 1 Alpha-2-macroglobulin receptor Apolipoprotein E receptor LRP1 A2MR APR protein protein human Homo sapiens Human MLTPPLLLLLPLLSALVAAAIDAPKTCSPKQFACRDQITCISKGWRCDGERDCPDGSDEAPEICPQSKAQRCQPNEHNCLGTELCVPMSRLCNGVQDCMDGSDEGPHCRELQGNCSRLGCQHHCVPTLDGPTCYCNSSFQLQADGKTCKDFDECSVYGTCSQLCTNTDGSFICGCVEGYLLQPDNRSCKAKNEPVDRPPVLLIANSQNILATYLSGAQVSTITPTSTRQTTAMDFSYANETVCWVHVGDSAAQTQLKCARMPGLKGFVDEHTINISLSLHHVEQMAIDWLTGNFYFVDDIDDRIFVCNRNGDTCVTLLDLELYNPKGIALDPAMGKVFFTDYGQIPKVERCDMDGQNRTKLVDSKIVFPHGITLDLVSRLVYWADAYLDYIEVVDYEGKGRQTIIQGILIEHLYGLTVFENYLYATNSDNANAQQKTSVIRVNRFNSTEYQVVTRVDKGGALHIYHQRRQPRVRSHACENDQYGKPGGCSDICLLANSHKARTCRCRSGFSLGSDGKSCKKPEHELFLVYGKGRPGIIRGMDMGAKVPDEHMIPIENLMNPRALDFHAETGFIYFADTTSYLIGRQKIDGTERETILKDGIHNVEGVAVDWMGDNLYWTDDGPKKTISVARLEKAAQTRKTLIEGKMTHPRAIVVDPLNGWMYWTDWEEDPKDSRRGRLERAWMDGSHRDIFVTSKTVLWPNGLSLDIPAGRLYWVDAFYDRIETILLNGTDRKIVYEGPELNHAFGLCHHGNYLFWTEYRSGSVYRLERGVGGAPPTVTLLRSERPPIFEIRMYDAQQQQVGTNKCRVNNGGCSSLCLATPGSRQCACAEDQVLDADGVTCLANPSYVPPPQCQPGEFACANSRCIQERWKCDGDNDCLDNSDEAPALCHQHTCPSDRFKCENNRCIPNRWLCDGDNDCGNSEDESNATCSARTCPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTCNNGRCININWRCDNDNDCGDNSDEAGCSHSCSSTQFKCNSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTHVCDPSVKFGCKDSARCISKAWVCDGDNDCEDNSDEENCESLACRPPSHPCANNTSVCLPPDKLCDGNDDCGDGSDEGELCDQCSLNNGGCSHNCSVAPGEGIVCSCPLGMELGPDNHTCQIQSYCAKHLKCSQKCDQNKFSVKCSCYEGWVLEPDGESCRSLDPFKPFIIFSNRHEIRRIDLHKGDYSVLVPGLRNTIALDFHLSQSALYWTDVVEDKIYRGKLLDNGALTSFEVVIQYGLATPEGLAVDWIAGNIYWVESNLDQIEVAKLDGTLRTTLLAGDIEHPRAIALDPRDGILFWTDWDASLPRIEAASMSGAGRRTVHRETGSGGWPNGLTVDYLEKRILWIDARSDAIYSARYDGSGHMEVLRGHEFLSHPFAVTLYGGEVYWTDWRTNTLAKANKWTGHNVTVVQRTNTQPFDLQVYHPSRQPMAPNPCEANGGQGPCSHLCLINYNRTVSCACPHLMKLHKDNTTCYEFKKFLLYARQMEIRGVDLDAPYYNYIISFTVPDIDNVTVLDYDAREQRVYWSDVRTQAIKRAFINGTGVETVVSADLPNAHGLAVDWVSRNLFWTSYDTNKKQINVARLDGSFKNAVVQGLEQPHGLVVHPLRGKLYWTDGDNISMANMDGSNRTLLFSGQKGPVGLAIDFPESKLYWISSGNHTINRCNLDGSGLEVIDAMRSQLGKATALAIMGDKLWWADQVSEKMGTCSKADGSGSVVLRNSTTLVMHMKVYDESIQLDHKGTNPCSVNNGDCSQLCLPTSETTRSCMCTAGYSLRSGQQACEGVGSFLLYSVHEGIRGIPLDPNDKSDALVPVSGTSLAVGIDFHAENDTIYWVDMGLSTISRAKRDQTWREDVVTNGIGRVEGIAVDWIAGNIYWTDQGFDVIEVARLNGSFRYVVISQGLDKPRAITVHPEKGYLFWTEWGQYPRIERSRLDGTERVVLVNVSISWPNGISVDYQDGKLYWCDARTDKIERIDLETGENREVVLSSNNMDMFSVSVFEDFIYWSDRTHANGSIKRGSKDNATDSVPLRTGIGVQLKDIKVFNRDRQKGTNVCAVANGGCQQLCLYRGRGQRACACAHGMLAEDGASCREYAGYLLYSERTILKSIHLSDERNLNAPVQPFEDPEHMKNVIALAFDYRAGTSPGTPNRIFFSDIHFGNIQQINDDGSRRITIVENVGSVEGLAYHRGWDTLYWTSYTTSTITRHTVDQTRPGAFERETVITMSGDDHPRAFVLDECQNLMFWTNWNEQHPSIMRAALSGANVLTLIEKDIRTPNGLAIDHRAEKLYFSDATLDKIERCEYDGSHRYVILKSEPVHPFGLAVYGEHIFWTDWVRRAVQRANKHVGSNMKLLRVDIPQQPMGIIAVANDTNSCELSPCRINNGGCQDLCLLTHQGHVNCSCRGGRILQDDLTCRAVNSSCRAQDEFECANGECINFSLTCDGVPHCKDKSDEKPSYCNSRRCKKTFRQCSNGRCVSNMLWCNGADDCGDGSDEIPCNKTACGVGEFRCRDGTCIGNSSRCNQFVDCEDASDEMNCSATDCSSYFRLGVKGVLFQPCERTSLCYAPSWVCDGANDCGDYSDERDCPGVKRPRCPLNYFACPSGRCIPMSWTCDKEDDCEHGEDETHCNKFCSEAQFECQNHRCISKQWLCDGSDDCGDGSDEAAHCEGKTCGPSSFSCPGTHVCVPERWLCDGDKDCADGADESIAAGCLYNSTCDDREFMCQNRQCIPKHFVCDHDRDCADGSDESPECEYPTCGPSEFRCANGRCLSSRQWECDGENDCHDQSDEAPKNPHCTSQEHKCNASSQFLCSSGRCVAEALLCNGQDDCGDSSDERGCHINECLSRKLSGCSQDCEDLKIGFKCRCRPGFRLKDDGRTCADVDECSTTFPCSQRCINTHGSYKCLCVEGYAPRGGDPHSCKAVTDEEPFLIFANRYYLRKLNLDGSNYTLLKQGLNNAVALDFDYREQMIYWTDVTTQGSMIRRMHLNGSNVQVLHRTGLSNPDGLAVDWVGGNLYWCDKGRDTIEVSKLNGAYRTVLVSSGLREPRALVVDVQNGYLYWTDWGDHSLIGRIGMDGSSRSVIVDTKITWPNGLTLDYVTERIYWADAREDYIEFASLDGSNRHVVLSQDIPHIFALTLFEDYVYWTDWETKSINRAHKTTGTNKTLLISTLHRPMDLHVFHALRQPDVPNHPCKVNNGGCSNLCLLSPGGGHKCACPTNFYLGSDGRTCVSNCTASQFVCKNDKCIPFWWKCDTEDDCGDHSDEPPDCPEFKCRPGQFQCSTGICTNPAFICDGDNDCQDNSDEANCDIHVCLPSQFKCTNTNRCIPGIFRCNGQDNCGDGEDERDCPEVTCAPNQFQCSITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDCTPRCDMDQFQCKSGHCIPLRWRCDADADCMDGSDEEACGTGVRTCPLDEFQCNNTLCKPLAWKCDGEDDCGDNSDENPEECARFVCPPNRPFRCKNDRVCLWIGRQCDGTDNCGDGTDEEDCEPPTAHTTHCKDKKEFLCRNQRCLSSSLRCNMFDDCGDGSDEEDCSIDPKLTSCATNASICGDEARCVRTEKAAYCACRSGFHTVPGQPGCQDINECLRFGTCSQLCNNTKGGHLCSCARNFMKTHNTCKAEGSEYQVLYIADDNEIRSLFPGHPHSAYEQAFQGDESVRIDAMDVHVKAGRVYWTNWHTGTISYRSLPPAAPPTTSNRHRRQIDRGVTHLNISGLKMPRGIAIDWVAGNVYWTDSGRDVIEVAQMKGENRKTLISGMIDEPHAIVVDPLRGTMYWSDWGNHPKIETAAMDGTLRETLVQDNIQWPTGLAVDYHNERLYWADAKLSVIGSIRLNGTDPIVAADSKRGLSHPFSIDVFEDYIYGVTYINNRVFKIHKFGHSPLVNLTGGLSHASDVVLYHQHKQPEVTNPCDRKKCEWLCLLSPSGPVCTCPNGKRLDNGTCVPVPSPTPPPDAPRPGTCNLQCFNGGSCFLNARRQPKCRCQPRYTGDKCELDQCWEHCRNGGTCAASPSGMPTCRCPTGFTGPKCTQQVCAGYCANNSTCTVNQGNQPQCRCLPGFLGDRCQYRQCSGYCENFGTCQMAADGSRQCRCTAYFEGSRCEVNKCSRCLEGACVVNKQSGDVTCNCTDGRVAPSCLTCVGHCSNGGSCTMNSKMMPECQCPPHMTGPRCEEHVFSQQQPGHIASILIPLLLLLLLVLVAGVVFWYKRRVQGAKGFQHQRMTNGAMNVEIGNPTYKMYEGGEPDDVGGLLDADFALDPDKPTNFTNPVYATLYMGGHGSRHSLASTDEKRELLGRGPEDEIGDPLA DIP protein Q07954 original sequence version: 159 5A11CC02FAB127BE apoe-lrp1-1 5 9 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Supp. Fig. 1 apoe-lrp1-2 3 9 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 2A, 2B EDTA (CHEBI:64755) Heparin (CHEBI:28304) Inhibition by EDTA (CHEBI:64755) suggests interaction is Ca2+ dependent LRPAP1 218-323 (P30533) calcium(2+)-lrp1-1 2 10 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q07954:p.Asp2797Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Fri Feb 01 16:42:10 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp2778Ala' Tue Jun 30 16:52:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp2797Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 3A apoe-lrp1-3 1 9 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ubiquitin tagged region fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q07954:p.Asp2797Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 19:33:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp2797Ala' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4, Table 1 apoe-ldlr-1 1 9 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ubiquitin tagged region fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4, Table 1 ldlr-lrpap1-1 1 8 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ubiquitin tagged region fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4, Table 1 lrp1-lrpap1-1 1 11 unspecified role unspecified role neutral component neutral component purified purified c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q07954:p.Asp2797Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 19:46:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp2797Ala' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ubiquitin tagged region fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 4, Table 1 apoe-lrp1-4 4 6 unspecified role unspecified role prey prey apoe binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dmpc binding region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q07954:p.[Asp2797Ala;Asp2840Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 18:53:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp2797Ala;Asp2840Ala]' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 76 72 association association Fig. 5, 6A, 6C, Table 2 LRPAP1 218-323 (P30533) EDTA (CHEBI:64755) Inhibition by EDTA showed interaction to be Ca2+-dependent apoe-lrp1-5 1 9 unspecified role unspecified role neutral component neutral component purified purified region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P02649:p.[Lys161Ala;Lys164Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:16:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys161Ala;Lys164Ala]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ubiquitin tagged region fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component purified purified c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4, Table 1 apoe-dimyristoylphosphatidyl choline-1 4 6 unspecified role unspecified role prey prey apoe binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dmpc binding region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role prey prey purified purified P01130:p.His211Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 16:59:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His211Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P01130:p.Asp170Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 17:55:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp170Ala' P01130:p.Gly219_Pro220delinsAspAla mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain GP DA Fri Feb 01 16:21:59 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly219Asp,Pro220Ala]' Tue Jun 30 17:38:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly219_Pro220delinsAspAla' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 96 92 association association Fig. 5, 6A, 6C Table 2 LRPAP1 218-323 (P30533) - GST tagged EDTA (CHEBI:64755) Inhibition by EDTA showed interaction to be Ca2+-dependent lrpap1-ldlr-1 4 7 unspecified role unspecified role prey prey purified purified P01130:p.His211Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 18:39:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His211Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P01130:p.Asp170Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 18:56:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp170Ala' P01130:p.Gly219_Pro220delinsAspAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain GP DA Fri Feb 01 16:14:34 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly219Asp,Pro220Ala]' Tue Jun 30 18:12:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly219_Pro220delinsAspAla' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 6B, Table 2 lrpap1-lrp1-1 4 8 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q07954:p.[Asp2797Ala;Asp2840Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 17:45:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp2797Ala;Asp2840Ala]' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 6B Table 2