IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional elements on SIRPalpha IgV domain mediate cell surface binding to CD47. Functional elements on SIRPalpha IgV domain mediate cell surface binding to CD47. Journal of molecular biology 2007 imex curation Liu Y., Tong Q., Zhou Y., Lee HW., Yang JJ., Bühring HJ., Chen YT., Ha B., Chen CX., Yang Y., Zen K. yliu@gsu.edu 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Liu Y., Tong Q., Zhou Y., Lee HW., Yang JJ., Bühring HJ., Chen YT., Ha B., Chen CX., Yang Y., Zen K. Origin of species of CD47 taken from the given back reference 11792697; Figure 2b, mutation Val57Met had been taken as Ala57Met, since SIRPalpha1 - P78324-4 being a variant of canonical SIRPalpha(Bit) - P78324; and as per the P78324-4, we do not have Valine at 57 yliu@gsu.edu Accepted 2019-SEP-18 AT 08:38 BST AT 08:38 BST by PPORRAS Journal of molecular biology 2007 imex curation sirb1_human Signal-regulatory protein beta-1 CD172 antigen-like family member B SIRPB1 protein protein human Homo sapiens Human MPVPASWPHLPSPFLLMTLLLGRLTGVAGEDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPLLVALLLGPKLLLVVGVSAIYICWKQKA C9C5E759514E212E cd47_human Leukocyte surface antigen CD47 CD47 MER6 Integrin-associated protein Antigenic surface determinant protein OA3 Protein MER6 protein protein human Homo sapiens Human MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE 5D20730A2632D550 p78324-4 Tyrosine-protein phosphatase non-receptor type substrate 1 SIRPA BIT MFR MYD1 PTPNS1 SHPS1 SIRP Brain Ig-like molecule with tyrosine-based activation motifs CD172 antigen-like family member A Inhibitory receptor SHPS-1 Macrophage fusion receptor MyD-1 antigen Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 p84 protein protein human Homo sapiens Human MEPAGPAPGRLGPLLCLLLAASCAWSGVAGEEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK AB4F162B7FF8D97A shps1_human Tyrosine-protein phosphatase non-receptor type substrate 1 Inhibitory receptor SHPS-1 Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 MyD-1 antigen Brain Ig-like molecule with tyrosine-based activation motifs Macrophage fusion receptor p84 CD172 antigen-like family member A SIRPA BIT MFR MYD1 SHPS1 SIRP PTPNS1 protein protein human Homo sapiens Human MEPAGPAPGRLGPLLCLLLAASCAWSGVAGEEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK 18D2FD04F6182AD0 cd47-sirpa-1 1 3 unspecified role unspecified role prey prey purified purified chlae-cos Chlorocebus aethiops cos cos 5 unspecified role unspecified role bait bait purified purified P78324:p.Ala57Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A M Tue Jun 30 16:49:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Met' P78324:p.Gln67Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q M Tue Jun 30 17:39:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Met' P78324:p.Met102Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M L Tue Jun 30 16:14:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met102Leu' P78324:p.Gln67Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q R Tue Jun 30 18:40:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Arg' P78324:p.Gln67Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q K Tue Jun 30 19:27:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Lys' P78324:p.Gln67Cys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q C Tue Jun 30 16:30:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Cys' P78324:p.Gln67Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q S Tue Jun 30 18:54:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Ser' P78324:p.Gln67Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q G Tue Jun 30 16:38:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Gly' P78324:p.Gln67Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q P Tue Jun 30 18:29:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Pro' P78324:p.Gln67Leu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q L Mon Apr 29 10:31:01 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Leu' Tue Jun 30 19:22:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Leu' P78324:p.Gln67Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q F Tue Jun 30 15:43:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Phe' P78324:p.Ala57Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 18:33:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Cys' P78324:p.Ala57Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A G Tue Jun 30 16:56:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Gly' P78324:p.Ala57Tyr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A Y Tue Jun 30 15:44:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Tyr' P78324:p.Ala57Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A R Tue Jun 30 17:07:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Arg' P78324:p.Ala57Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A D Tue Jun 30 17:39:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Asp' P78324:p.Ala57Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A S Tue Jun 30 19:01:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Ser' P78324:p.Ala57Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 17:07:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Thr' P78324:p.Ala57Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 16:14:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Val' P78324:p.Ala57Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A L Tue Jun 30 18:53:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Leu' P78324:p.Ala57Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A P Tue Jun 30 19:49:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Pro' P78324:p.Ala57Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A W Tue Jun 30 17:53:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Trp' P78324:p.Ala57Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A F Tue Jun 30 19:52:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Phe' chlae-cos Chlorocebus aethiops cos cos direct interaction direct interaction Figure 1b,Figure 2a, 2b, Figure 3, Figure 4 cd47-sirpb1-1 1 4 unspecified role unspecified role prey prey purified purified P78324-4:p.Ala57Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A M Tue Jun 30 20:08:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala57Met' chlae-cos Chlorocebus aethiops cos cos 3 unspecified role unspecified role bait bait chlae-cos Chlorocebus aethiops cos cos direct interaction direct interaction Figure 1, Figure 2a cd47-sirpb1-2 1 3 unspecified role unspecified role prey prey purified purified chlae-cos Chlorocebus aethiops cos cos 2 unspecified role unspecified role bait bait purified purified O00241:p.[Met56Val;Met66Gln;Leu101Met] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain L M certain certain sequence position certain certain certain sequence position certain M V certain certain sequence position certain certain certain sequence position certain M Q Tue Jun 30 18:25:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met56Val;Met66Gln;Leu101Met]' O00241:p.[Met56Val;Met66Gln;Ala73Pro] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain M Q certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain M V Tue Jun 30 18:17:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met56Val;Met66Gln;Ala73Pro]' O00241:p.[Met56Val;Met66Gln] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain M V certain certain sequence position certain certain certain sequence position certain M Q Tue Jun 30 19:32:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met56Val;Met66Gln]' chlae-cos Chlorocebus aethiops cos cos direct interaction direct interaction Figure 8 p.Met56Val; p.Met66Gln mutations were not found to cause interaction between CD47 and SIRPB1