IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Multiple epiphyseal dysplasia mutations in MATN3 cause misfolding of the A-domain and prevent secretion of mutant matrilin-3. Multiple epiphyseal dysplasia mutations in MATN3 cause misfolding of the A-domain and prevent secretion of mutant matrilin-3. Human mutation 2005 imex curation Cotterill SL., Jackson GC., Leighton MP., Wagener R., Mäkitie O., Cole WG., Briggs MD. "A" domain sequence of Matrilin-3 found from primers. mike.briggs@manchester.ac.uk 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Cotterill SL., Jackson GC., Leighton MP., Wagener R., Mäkitie O., Cole WG., Briggs MD. CHO-B2 cells; a clone of CHO-K1 cells selected for very low cell surface expression of alpha5-beta1 integrin. Crosslinker was dithiobis[succinimidyl propionate] (DSP), followed by an IP using an anti-ERp72 antibody. "A" domain sequence of Matrilin-3 found from primers. mike.briggs@manchester.ac.uk Accepted 2019-SEP-16 AT 10:09 BST AT 10:09 BST by PPORRAS Human mutation 2005 Only protein-protein interactions imex curation imex curation pdia4_human Protein disulfide-isomerase A4 Endoplasmic reticulum resident protein 72 PDIA4 ERP70 ERP72 Endoplasmic reticulum resident protein 70 protein protein human Homo sapiens Human MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL 1919C2AE12CD2684 matn3_human Matrilin-3 MATN3 protein protein human Homo sapiens Human MPRPAPARRLPGLLLLLWPLLLLPSAAPDPVARPGFRRLETRGPGGSPGRRPSPAAPDGAPASGTSEPGRARGAGVCKSRPLDLVFIIDSSRSVRPLEFTKVKTFVSRIIDTLDIGPADTRVAVVNYASTVKIEFQLQAYTDKQSLKQAVGRITPLSTGTMSGLAIQTAMDEAFTVEAGAREPSSNIPKVAIIVTDGRPQDQVNEVAARAQASGIELYAVGVDRADMASLKMMASEPLEEHVFYVETYGVIEKLSSRFQETFCALDPCVLGTHQCQHVCISDGEGKHHCECSQGYTLNADKKTCSALDRCALNTHGCEHICVNDRSGSYHCECYEGYTLNEDRKTCSAQDKCALGTHGCQHICVNDRTGSHHCECYEGYTLNADKKTCSVRDKCALGSHGCQHICVSDGAASYHCDCYPGYTLNEDKKTCSATEEARRLVSTEDACGCEATLAFQDKVSSYLQRLNTKLDDILEKLKINEYGQIHR 688847BCC791B331 pdia4-matn3-1 1 2 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed A_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range murine_kappa_light chain_leader _sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O15232:p.Val194Asp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain V D Tue Jun 30 18:49:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val194Asp' O15232:p.Arg121Trp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain R W Tue Jun 30 17:05:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg121Trp' O15232:p.Ala219Asp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A D Tue Jun 30 19:35:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala219Asp' O15232:p.Glu252Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 16:56:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu252Lys' physical association physical association Figure 3A