UniProt UniProt http://www.uniprot.org Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Neurobiology of disease 2003 imex curation Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease georges.levesque@crsfa.ulaval.ca 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP taken from previous work by the same author georges.levesque@crsfa.ulaval.ca Accepted 2019-AUG-19 AT 08:51 BST AT 08:51 BST by MGT Neurobiology of disease 2003 Only protein-protein interactions imex curation imex curation Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Neurobiology of disease 2003 imex curation Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease georges.levesque@crsfa.ulaval.ca 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP taken from previous work by the same author georges.levesque@crsfa.ulaval.ca Accepted 2019-AUG-19 AT 08:51 BST AT 08:51 BST by MGT Neurobiology of disease 2003 Only protein-protein interactions imex curation imex curation Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Neurobiology of disease 2003 imex curation Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease georges.levesque@crsfa.ulaval.ca 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP taken from previous work by the same author georges.levesque@crsfa.ulaval.ca Accepted 2019-AUG-19 AT 08:51 BST AT 08:51 BST by MGT Neurobiology of disease 2003 Only protein-protein interactions imex curation imex curation Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Neurobiology of disease 2003 imex curation Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease georges.levesque@crsfa.ulaval.ca 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP taken from previous work by the same author georges.levesque@crsfa.ulaval.ca Accepted 2019-AUG-19 AT 08:51 BST AT 08:51 BST by MGT Neurobiology of disease 2003 Only protein-protein interactions imex curation imex curation Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Presenilin-1 interacts directly with the beta-site amyloid protein precursor cleaving enzyme (BACE1). Neurobiology of disease 2003 imex curation Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease georges.levesque@crsfa.ulaval.ca 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hébert SS., Bourdages V., Godin C., Ferland M., Carreau M., Lévesque G. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP taken from previous work by the same author georges.levesque@crsfa.ulaval.ca Accepted 2019-AUG-19 AT 08:51 BST AT 08:51 BST by MGT Neurobiology of disease 2003 Only protein-protein interactions imex curation imex curation psn1_human Presenilin-1 PSEN1 AD3 PS1 PSNL1 Protein S182 protein protein human Homo sapiens Human MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSRQVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI DIP protein P49768 original sequence version: 106 DIP protein P49768-1 original sequence version: 144 DIP protein P49768 original sequence version: 186 5E0F451EF82BCF20 bace1_human Beta-secretase 1 BACE KIAA1149 Beta-site amyloid precursor protein cleaving enzyme 1 Membrane-associated aspartic protease 2 Memapsin-2 Aspartyl protease 2 BACE1 protein protein human Homo sapiens Human MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK DIP protein P56817 original sequence version: 161 377CE4C824ACEF05 377CE4C824ACEF05 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 bace1-psen1-1 2 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Fig. 1 psen1-bace1-1 2 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Fig. 1 app-bace1-6 2 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Fig. 1 psen1-bace1-2 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 2B bace1-psen1-2 5 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Fig. 2C bace1-psen1-3 3 7 unspecified role unspecified role bait bait purified purified n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 2D bace1-psen1-4 4 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Fig. 3C