IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. Proceedings of the National Academy of Sciences of the United States of America 2003 imex curation Nguyen BD., Abbott KL., Potempa K., Kobor MS., Archambault J., Greenblatt J., Legault P., Omichinski JG. The expression system used for RAP74 was determined from a different publication (PMID:12578358). jim@bmbiris. bmb.uga.edu 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Nguyen BD., Abbott KL., Potempa K., Kobor MS., Archambault J., Greenblatt J., Legault P., Omichinski JG. The expression system used for RAP74 was determined from a different publication (PMID:12578358). jim@bmbiris. bmb.uga.edu Accepted 2019-OCT-09 AT 11:17 BST AT 11:17 BST by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2003 Only protein-protein interactions imex curation imex curation NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. Proceedings of the National Academy of Sciences of the United States of America 2003 imex curation Nguyen BD., Abbott KL., Potempa K., Kobor MS., Archambault J., Greenblatt J., Legault P., Omichinski JG. The expression system used for RAP74 was determined from a different publication (PMID:12578358). jim@bmbiris. bmb.uga.edu 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nguyen BD., Abbott KL., Potempa K., Kobor MS., Archambault J., Greenblatt J., Legault P., Omichinski JG. The expression system used for RAP74 was determined from a different publication (PMID:12578358). jim@bmbiris. bmb.uga.edu Accepted 2019-OCT-09 AT 11:17 BST AT 11:17 BST by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2003 Only protein-protein interactions imex curation imex curation t2fa_human General transcription factor IIF subunit 1 Transcription initiation factor IIF subunit alpha GTF2F1 RAP74 Transcription initiation factor RAP74 General transcription factor IIF 74 kDa subunit protein protein human Homo sapiens Human MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAAKRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE DIP protein P35269 original sequence version: 109 DIP protein P35269 original sequence version: 171 DIP protein P35269 original sequence version: 164 1032B04A36BDC24F ctdp1_human RNA polymerase II subunit A C-terminal domain phosphatase CTDP1 FCP1 TFIIF-associating CTD phosphatase protein protein human Homo sapiens Human MEVPAAGRVPAEGAPTAAVAEVRCPGPAPLRLLEWRVAAGAAVRIGSVLAVFEAAASAQSSGASQSRVASGGCVRPARPERRLRSERAGVVRELCAQPGQVVAPGAVLVRLEGCSHPVVMKGLCAECGQDLTQLQSKNGKQQVPLSTATVSMVHSVPELMVSSEQAEQLGREDQQRLHRNRKLVLMVDLDQTLIHTTEQHCQQMSNKGIFHFQLGRGEPMLHTRLRPHCKDFLEKIAKLYELHVFTFGSRLYAHTIAGFLDPEKKLFSHRILSRDECIDPFSKTGNLRNLFPCGDSMVCIIDDREDVWKFAPNLITVKKYVYFQGTGDMNAPPGSRESQTRKKVNHSRGTEVSEPSPPVRDPEGVTQAPGVEPSNGLEKPARELNGSEAATPRDSPRPGKPDERDIWPPAQAPTSSQELAGAPEPQGSCAQGGRVAPGQRPAQGATGTDLDFDLSSDSESSSESEGTKSSSSASDGESEGKRGRQKPKAAPEGAGALAQGSSLEPGRPAAPSLPGEAEPGAHAPDKEPELGGQEEGERDGLCGLGNGCADRKEAETESQNSELSGVTAGESLDQSMEEEEEEDTDEDDHLIYLEEILVRVHTDYYAKYDRYLNKEIEEAPDIRKIVPELKSKVLADVAIIFSGLHPTNFPIEKTREHYHATALGAKILTRLVLSPDAPDRATHLIAARAGTEKVLQAQECGHLHVVNPDWLWSCLERWDKVEEQLFPLRDDHTKAQRENSPAAFPDREGVPPTALFHPMPVLPKAQPGPEVRIYDSNTGKLIRTGARGPPAPSSSLPIRQEPSSFRAVPPPQPQMFGEELPDAQDGEQPGPSRRKRQPSMSETMPLYTLCKEDLESMDKEVDDILGEGSDDSDSEKRRPEEQEEEPQPRKPGTRRERTLGAPASSERSAAGGRGPRGHKRKLNEEDAASESSRESSNEDEGSSSEADEMAKALEAELNDLM 62B88FFD9CE4B157 ctdp1-gtf2f1-1 1 4 unspecified role unspecified role neutral component neutral component purified purified cterFCP sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13C radiolabelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 3 unspecified role unspecified role neutral component neutral component purified purified cterRAP74 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13C radiolabelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 11 7 direct interaction direct interaction Fig.1-3, table 1 ctdp1-gtf2f1-2 2 3 unspecified role unspecified role prey prey purified purified cterRAP74 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 4 unspecified role unspecified role bait bait purified purified GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cterFCP sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9Y5B0:p.Glu956_Leu957delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain EL AA Tue Jun 30 17:26:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu956_Leu957delinsAlaAla' ecobd Escherichia coli (strain B / BL21-DE3) 19 16 direct interaction direct interaction Fig.4B