IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Molecular cell 2003 imex curation Selenko P., Gregorovic G., Sprangers R., Stier G., Rhani Z., Krämer A., Sattler M. sattler@embl.de 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Selenko P., Gregorovic G., Sprangers R., Stier G., Rhani Z., Krämer A., Sattler M. sattler@embl.de Accepted 2020-MAR-11 AT 09:44 GMT AT 09:44 GMT by PPORRAS Molecular cell 2003 Only protein-protein interactions imex curation imex curation Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Molecular cell 2003 imex curation Selenko P., Gregorovic G., Sprangers R., Stier G., Rhani Z., Krämer A., Sattler M. sattler@embl.de 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Selenko P., Gregorovic G., Sprangers R., Stier G., Rhani Z., Krämer A., Sattler M. sattler@embl.de Accepted 2020-MAR-11 AT 09:44 GMT AT 09:44 GMT by PPORRAS Molecular cell 2003 Only protein-protein interactions imex curation imex curation sf01_human Splicing factor 1 Zinc finger protein 162 Transcription factor ZFM1 Zinc finger gene in MEN1 locus Mammalian branch point-binding protein SF1 ZFM1 ZNF162 protein protein human Homo sapiens Human MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGPPPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN DIP protein Q15637 original sequence version: 73 DIP protein Q15637-1 original sequence version: 138 EEBC6A02B29DAE4D u2af2_human Splicing factor U2AF 65 kDa subunit U2AF2 U2AF65 U2 snRNP auxiliary factor large subunit U2 auxiliary factor 65 kDa subunit protein protein human Homo sapiens Human MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW DIP protein P26368 original sequence version: 86 DIP protein P26368 original sequence version: 151 DIP protein P26368-1 original sequence version: 151 DIP protein P26368 original sequence version: 124 26AD271CD8FC6211 sf1-u2af2-16 1 4 unspecified role unspecified role neutral component neutral component purified purified 13C radiolabelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RRM3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 3 unspecified role unspecified role neutral component neutral component purified purified Binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13C radiolabelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 8 direct interaction direct interaction Fig.2B-D, Fig.3A-C The quoted Kd value is an upper estimate, the authors claim that the true Kd<1uM. sf1-u2af2-17 2 3 unspecified role unspecified role prey prey purified purified N-terminal half sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His6 tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobd Escherichia coli (strain B / BL21-DE3) 4 unspecified role unspecified role bait bait purified purified RRM3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 3 competitor competitor competitor unspecified role unspecified role purified purified Binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His6 tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 19 16 22 19 direct interaction direct interaction Fig.2A While the 1-25 residue SF1 construct is a potent competitor for the RRM3-SF1 interaction, a delta2-28 SF1 construct was unable to compete in a separate pull down experiment (see figure 2A of the paper). u2af2-sf1-4 2 4 unspecified role unspecified role bait bait purified purified P26368:p.Glu396_Glu397delinsGlyAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain EE GA Wed Sep 18 13:41:55 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu396_Glu397GlyAla' Tue Jun 30 17:46:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu396_Glu397delinsGlyAla' P26368:p.Glu396_Glu397delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain EE AA Wed Sep 18 13:47:07 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu396_Glu397AlaAla' Tue Jun 30 19:35:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu396_Glu397delinsAlaAla' P26368:p.Glu387_Glu388delinsArgArg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain EE RR Tue Jun 30 17:11:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu387_Glu388delinsArgArg' P26368:p.Glu396_Glu397delinsLysLys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain EE KK Wed Sep 18 13:26:53 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu396_Glu397LysLys' Tue Jun 30 15:44:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu396_Glu397delinsLysLys' P26368:p.Asp391_Glu394delinsAlaAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain DDEE AAAA Tue Jun 30 20:19:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp391_Glu394delinsAlaAlaAlaAla' P26368:p.Asp391_Glu394delinsArgArgLysLys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain DDEE RRKK Tue Jun 30 19:54:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp391_Glu394delinsArgArgLysLys' RRM3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P26368:p.Phe454Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F A Tue Jun 30 20:04:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe454Ala' P26368:p.Phe454Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F Y Tue Jun 30 19:20:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe454Tyr' P26368:p.Glu387_Glu388delinsGlyAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain EE GA Tue Jun 30 17:50:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu387_Glu388delinsGlyAla' ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey purified purified N-terminal half sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q15637:p.Lys15Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 18:46:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys15Glu' Q15637:p.Arg17Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 16:40:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg17Glu' Q15637:p.Arg19Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 15:38:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg19Glu' Q15637:p.Arg21Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R K Tue Jun 30 17:12:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg21Lys' Q15637:p.Arg21Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R D Tue Jun 30 20:00:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg21Asp' Q15637:p.Trp22Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain W F Tue Jun 30 16:58:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp22Phe' Q15637:p.Trp22Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain W A Tue Jun 30 19:34:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp22Ala' Q15637:p.Asn23Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 17:35:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn23Ala' Q15637:p.Gln24Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 17:29:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln24Ala' Q15637:p.Lys15_Arg17delinsGluGluAsp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain KKR EED Tue Jun 30 18:22:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys15_Arg17delinsGluGluAsp' Q15637:p.Lys16_Lys18delinsAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain KRK AAA Tue Jun 30 17:24:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys16_Lys18delinsAlaAlaAla' Q15637:p.Lys16_Lys18delinsGluAspGlu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain KRK EDE Tue Jun 30 17:28:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys16_Lys18delinsGluAspGlu' ecoli Escherichia coli (strain K12) 38 32 direct interaction direct interaction Fig.3D and E