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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: ketimine and pyridoxamine phosphate forms.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="12269802" refType="primary-reference" refTypeAc="MI:0358"/>
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          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: ketimine and pyridoxamine phosphate forms.</attribute>
            <attribute name="journal" nameAc="MI:0885">Biochemistry</attribute>
            <attribute name="publication year" nameAc="MI:0886">2002</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Yennawar NH., Conway ME., Yennawar HP., Farber GK., Hutson SM.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">nhy1@psu.edu</attribute>
            <attribute name="author-announcement">13-Nov-2020: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Yennawar NH., Conway ME., Yennawar HP., Farber GK., Hutson SM.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">nhy1@psu.edu</attribute>
          <attribute name="accepted">Accepted 2020-OCT-13 AT 11:05 BST AT 11:05 BST by LIVIAP</attribute>
          <attribute name="journal" nameAc="MI:0885">Biochemistry</attribute>
          <attribute name="publication year" nameAc="MI:0886">2002</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>bcat2_human</shortLabel>
          <fullName>Branched-chain-amino-acid aminotransferase, mitochondrial</fullName>
          <alias type="gene name" typeAc="MI:0301">BCAT2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">BCATM</alias>
          <alias type="gene name synonym" typeAc="MI:0302">BCT2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">ECA40</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Placental protein 18</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="O15382" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q9BUU6" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="B2RB87" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q96KG1" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="O00269" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q9BTB6" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000322991" version="SP_197" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3926101" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001271254.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001158245.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001181.2"/>
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          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000316273" version="SP_197" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004084" version="SP_197"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009098" version="SP_197"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0010817" version="SP_197"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1EKP" version="SP_197"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5BWW" version="SP_197"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5I5S" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5I5T" version="SP_197"/>
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          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-70895" version="SP_197"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV</sequence>
        <attributeList>
          <attribute name="crc64">EC07047264B190DA</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-28435-1">
        <names>
          <shortLabel>bcat2-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="wwpdb" dbAc="MI:0805" id="1KT8" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="imex" dbAc="MI:0670" id="IM-28435-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
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                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
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                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
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              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
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              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="5">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-26367844" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
                  </names>
                  <xref>
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                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
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                      </names>
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                    </startStatus>
                    <begin position="28"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
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                      </xref>
                    </endStatus>
                    <end position="392"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig 1, Table 1</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>