DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM reveals two distinct serotonin-bound conformations of full-length 5-HT3A receptor. Cryo-EM reveals two distinct serotonin-bound conformations of full-length 5-HT3A receptor. Nature 2018 imex curation Basak S., Gicheru Y., Rao S., Sansom MSP., Chakrapani S. sudha.chakrapani@case.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Basak S., Gicheru Y., Rao S., Sansom MSP., Chakrapani S. sudha.chakrapani@case.edu Accepted 2019-JAN-21 AT 13:06 GMT AT 13:06 GMT by ORCHARD Nature 2018 imex curation e9qlc0_mouse Htr3a protein protein mouse Mus musculus Mouse MRLCIPQVLLALFLSMLTAPGEGSRRRATQARDTTQPALLRLSDHLLANYKKGVRPVRDWRKPTTVSIDVIMYAILNVDEKNQVLTTYIWYRQYWTDEFLQWTPEDFDNVTKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYVHHRGEVQNYKPLQLVTACSLDIYNFPFDVQNCSLTFTSWLHTIQDINITLWRSPEEVRSDKSIFINQGEWELLEVFPQFKEFSIDISNSYAEMKFYVIIRRRPLFYAVSLLLPSIFLMVVDIVGFCLPPDSGERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRLVHKQDLQRPVPDWLRHLVLDRIAWILCLGEQPMAHRPPATFQANKTDDCSGSDLLPAMGNHCSHVGGPQDLEKTPRGRGSPLPPPREASLAVRGLLQELSSIRHFLEKRDEMREVARDWLRVGYVLDRLLFRIYLLAVLAYSITLVTLWSIWHYS 91FB42F7B252DC1A htr3a-9 1 2 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Strep tag Strep tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 1D4 tag 1D4 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 1D4 tag is not in the 'feature-type' drop down. Hence, just added tag as the feature type direct interaction direct interaction Fig 1, Extended Data Table 1