UniProt UniProt http://www.uniprot.org Copper mediated amyloid-β binding to Transthyretin. Copper mediated amyloid-β binding to Transthyretin. Scientific reports 2018 imex curation Ciccone L., Fruchart-Gaillard C., Mourier G., Savko M., Nencetti S., Orlandini E., Servent D., Stura EA., Shepard W. Alzheimers - Interactions investigated in the context of Alzheimers disease william.shepard@synchrotron-soleil.fr 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ciccone L., Fruchart-Gaillard C., Mourier G., Savko M., Nencetti S., Orlandini E., Servent D., Stura EA., Shepard W. Alzheimers - Interactions investigated in the context of Alzheimers disease APP-containing crystal wasn't submitted to PDB because of quality but as we are only using it as evidence of the interaction I decided quality didn't matter so much. It is fully described in table and text. william.shepard@synchrotron-soleil.fr Accepted 2019-JAN-21 AT 16:51 GMT AT 16:51 GMT by PPORRAS Scientific reports 2018 imex curation Cannot use agonist/stimulator here as no stimulation involved so have added as interactors. Have not added R-factors from table as those submitted bear no relation to those in table, also I can't work out which is R-working and which R-free.; Have made Copper a stimulator (not agonist - this is used for whole cell assays) as there is some binding shown in its absence. Copper mediated amyloid-β binding to Transthyretin. Copper mediated amyloid-β binding to Transthyretin. Scientific reports 2018 imex curation Ciccone L., Fruchart-Gaillard C., Mourier G., Savko M., Nencetti S., Orlandini E., Servent D., Stura EA., Shepard W. Alzheimers - Interactions investigated in the context of Alzheimers disease william.shepard@synchrotron-soleil.fr 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Ciccone L., Fruchart-Gaillard C., Mourier G., Savko M., Nencetti S., Orlandini E., Servent D., Stura EA., Shepard W. Alzheimers - Interactions investigated in the context of Alzheimers disease william.shepard@synchrotron-soleil.fr Accepted 2019-JAN-21 AT 16:51 GMT AT 16:51 GMT by PPORRAS Scientific reports 2018 imex curation Cannot use agonist/stimulator here as no stimulation involved so have added as interactors. Have not added R-factors from table as those submitted bear no relation to those in table, also I can't work out which is R-working and which R-free.; Have made Copper a stimulator (not agonist - this is used for whole cell assays) as there is some binding shown in its absence. tthy_human Transthyretin Prealbumin TBPA ATTR TTR PALB protein protein human Homo sapiens Human MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE DIP protein P02766 original sequence version: 114 DIP protein P02766 original sequence version: 163 DIP protein P02766 original sequence version: 174 3A6AEBCBBA56BC44 copper(2+) Copper (Cu2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) iron(3+) iron(3+) FE (III) ION Fe(III) Fe3+ Fe(3+) Ferric ion ferric iron Iron(3+) iron, ion (Fe(3+)) iron(III) cation iron(3+) iron(3+) ion small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) manganese(2+) Manganese (2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 app-ttr-1 2 3 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Binding is copper-dependent Figs 1B, 1C Copper 2+ (CHEBI:29036) app-ttr-2 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 2.14A copper(2+)-ttr-1 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figs 1B, 1C manganese(2+)-ttr-1 1 6 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figs 1B, 1C iron2plus-ttr-1 1 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Crystals were soaked with Cu2+ (5N7C), Mn2+ (5N62) or Fe2+ (5N5Q) Figs 1B, 1C