DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the human voltage-gated sodium channel Nav1.4 in complex with β1. Structure of the human voltage-gated sodium channel Nav1.4 in complex with β1. Science (New York, N.Y.) 2018 imex curation Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N., Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N. nyan@princeton.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N., Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N. nyan@princeton.edu Accepted 2018-NOV-30 AT 14:24 GMT AT 14:24 GMT by ORCHARD Science (New York, N.Y.) 2018 imex curation Structure of the human voltage-gated sodium channel Nav1.4 in complex with β1. Structure of the human voltage-gated sodium channel Nav1.4 in complex with β1. Science (New York, N.Y.) 2018 imex curation Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N., Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N. nyan@princeton.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N., Pan X., Li Z., Zhou Q., Shen H., Wu K., Huang X., Chen J., Zhang J., Zhu X., Lei J., Xiong W., Gong H., Xiao B., Yan N. nyan@princeton.edu Accepted 2018-NOV-30 AT 14:24 GMT AT 14:24 GMT by ORCHARD Science (New York, N.Y.) 2018 imex curation q07699-1 Sodium channel subunit beta-1 SCN1B Beta-1 protein protein human Homo sapiens Human MGRLLALVVGAALVSSACGGCVEVDSETEAVYGMTFKILCISCKRRSETNAETFTEWTFRQKGTEEFVKILRYENEVLQLEEDERFEGRVVWNGSRGTKDLQDLSIFITNVTYNHSGDYECHVYRLLFFENYEHNTSVVKKIHIEVVDKANRDMASIVSEIMMYVLIVVLTIWLVAEMIYCYKKIAAATETAAQENASEYLAITSESKENCTGVQVAE 09B812FA3F9E9018 scn4a_human Sodium channel protein type 4 subunit alpha SCN4A SkM1 Sodium channel protein skeletal muscle subunit alpha Sodium channel protein type IV subunit alpha Voltage-gated sodium channel subunit alpha Nav1.4 protein protein human Homo sapiens Human MARPSLCTLVPLGPECLRPFTRESLAAIEQRAVEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPMIYGDPPPEVIGIPLEDLDPYYSNKKTFIVLNKGKAIFRFSATPALYLLSPFSVVRRGAIKVLIHALFSMFIMITILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCVDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNTTWYSNDTWYGNDTWYGNEMWYGNDSWYANDTWNSHASWATNDTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKTGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDKEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEADGDPAHGKDCNGSLDTSQGEKGAPRQSSSGDSGISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWLKFKNIIHLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLTVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIALDCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKLGIGFAKAFLLGLLHGKILSPKDIMLSLGEADGAGEAGEAGETAPEDEKKEPPEEDLKKDNHILNHMGLADGPPSSLELDHLNFINNPYLTIQVPIASEESDLEMPTEEETDTFSEPEDSKKPPQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGEQPEECFTEACVQRWPCLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIRTILEYADKVFTYIFIMEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDLVTKQAFDITIMILICLNMVTMMVETDNQSQLKVDILYNINMIFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSVKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIAYSRLSDFVDTLQEPLRIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSMKQASYMYRHSHDGSGDDAPEKEGLLANTMSKMYGHENGNSSSPSPEEKGEAGDAGPTMGLMPISPSDTAWPPAPPPGQTVRPGVKESLV interactionMixImport_1519598262712 scn1b-scn4a-1 2 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified twin strep tag twin strep tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Figure 1B scn1b-scn4a-2 1 3 predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role unspecified role unspecified role purified purified human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role unspecified role unspecified role purified purified twin strep tag twin strep tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Figure 1A