DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of a mitochondrial calcium uniporter. Cryo-EM structure of a mitochondrial calcium uniporter. Science (New York, N.Y.) 2018 imex curation Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu Accepted 2018-AUG-20 AT 11:06 BST AT 11:06 BST by ORCHARD Science (New York, N.Y.) 2018 imex curation Cryo-EM structure of a mitochondrial calcium uniporter. Cryo-EM structure of a mitochondrial calcium uniporter. Science (New York, N.Y.) 2018 imex curation Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu Accepted 2018-AUG-20 AT 11:06 BST AT 11:06 BST by ORCHARD Science (New York, N.Y.) 2018 imex curation Cryo-EM structure of a mitochondrial calcium uniporter. Cryo-EM structure of a mitochondrial calcium uniporter. Science (New York, N.Y.) 2018 imex curation Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink predetermined predetermined participant predetermined Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. Crosslinker - BS3 (bis(sulfosuccinimidyl)suberate) seok-yong.lee@duke.edu,glander@scripps.edu Accepted 2018-AUG-20 AT 11:06 BST AT 11:06 BST by ORCHARD Science (New York, N.Y.) 2018 imex curation q7s4i4_neucr Calcium uniporter protein NCU08166 protein protein neucr Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) MNCVRMRLQCRLMPSSNTLARWCLESPQKNLTLHRPRISNVALRQASTSVSPKTRETEAEAKAKKLDQKRLDEHEEEVRAREQQVRRPWHREGADKPPVEGNADPIAKGKLLTTPTRLLKLILPLPLRVEKDQKNNGRNNEYGRSISLNSDIQPLALLIHPQQPLSYVERLIQAELPPVVENGQEKIPNVYFRAEDSEQGDQKPTSRAEARSKDDGGEPSEYNTNLSHVASYSGLGHRGPKRSSQDKRWVRWSSSTEMGDFIRDAARGREFAIEIEGYNIEMRVSVPSFGDRTYYMRQRLRKMSSEIDGLAKIKHECDLLAHRSAHRLAKGGFGLLAGWWGVVYYVTFHTEFGWDLVEPVTYLAGLTTIMGGYLWFLYINKDLSYKAAMNVTVSRRQHALYEMKGFDIERWEQLVQDANALRREIRVIAVEYDVDWDETRDVGEDVKDVLDEERSRRDDEHRSIEKEKDEKFTEDEKRKRKKDKESKETSGDS 3624FBACCB4D2EA3 hjj-1 2 4 unspecified role unspecified role neutral component neutral component purified purified Binding Region Binding Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q7S4I4:p.Tyr232Ala Tyr232Ala mutation mutation certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 15:48:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr232Ala' direct interaction direct interaction Figure 1, Table S1 q7s4i4_neucr-1 3 4 unspecified role unspecified role neutral component neutral component purified purified Binding Region Binding Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q7S4I4:p.Tyr232Ala Tyr232Ala mutation mutation certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 15:54:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr232Ala' direct interaction direct interaction Fig. S2 (A) q7s4i4_neucr-2 1 4 unspecified role unspecified role neutral component neutral component purified purified Binding Region Binding Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q7S4I4:p.Tyr232Ala Tyr232Ala mutation mutation certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 18:59:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr232Ala' direct interaction direct interaction Fig.S2.(B)