IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ MiR-216a exerts tumor-suppressing functions in renal cell carcinoma by targeting TLR4. MiR-216a exerts tumor-suppressing functions in renal cell carcinoma by targeting TLR4. American journal of cancer research 2017 imex curation Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X., Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X. xdli1010@163.com 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-786_o Homo sapiens renal cell adenocarcinoma 786_o Human renal cell adenocarcinoma cell line CRL-1932 is the ATCC identifier. ATCC identifier is CRL-1932. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X., Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X. xdli1010@163.com Accepted 2018-JUL-04 AT 11:05 BST AT 11:05 BST by LIVIAP American journal of cancer research 2017 Only protein-protein interactions imex curation imex curation MiR-216a exerts tumor-suppressing functions in renal cell carcinoma by targeting TLR4. MiR-216a exerts tumor-suppressing functions in renal cell carcinoma by targeting TLR4. American journal of cancer research 2017 imex curation Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X., Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X. xdli1010@163.com 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-caki_1 Homo sapiens caki_1 Human clear-cell renal-cell carcinoma cell line mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X., Wang W., Zhao E., Yu Y., Geng B., Zhang W., Li X. xdli1010@163.com Accepted 2018-JUL-04 AT 11:05 BST AT 11:05 BST by LIVIAP American journal of cancer research 2017 Only protein-protein interactions imex curation imex curation mrna_tlr4 toll like receptor 4 mRNA mrna messenger rna mRNA mrna human Homo sapiens Human TAGCTTCCTCTTGCTGTTTCTTTAGCCACTGGTCTGCAGGCGTTTTCTTCTTCTAACTTCCTCTCCTGTGACAAAAGAGATAACTATTAGAGAAACAAAAGTCCAGAATGCTAAGGTTGCCGCTTTCACTTCCTCTCACCCTTTAGCCCAGAACTGCTTTGAATACACCAATTGCTGTGGGGCGGCTCGAGGAAGAGAAGACACCAGTGCCTCAGAAACTGCTCGGTCAGACGGTGATAGCGAGCCACGCATTCACAGGGCCACTGCTGCTCACAGAAGCAGTGAGGATGATGCCAGGATGATGTCTGCCTCGCGCCTGGCTGGGACTCTGATCCCAGCCATGGCCTTCCTCTCCTGCGTGAGACCAGAAAGCTGGGAGCCCTGCGTGGAGGTGGTTCCTAATATTACTTATCAATGCATGGAGCTGAATTTCTACAAAATCCCCGACAACCTCCCCTTCTCAACCAAGAACCTGGACCTGAGCTTTAATCCCCTGAGGCATTTAGGCAGCTATAGCTTCTTCAGTTTCCCAGAACTGCAGGTGCTGGATTTATCCAGGTGTGAAATCCAGACAATTGAAGATGGGGCATATCAGAGCCTAAGCCACCTCTCTACCTTAATATTGACAGGAAACCCCATCCAGAGTTTAGCCCTGGGAGCCTTTTCTGGACTATCAAGTTTACAGAAGCTGGTGGCTGTGGAGACAAATCTAGCATCTCTAGAGAACTTCCCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCTCACAATCTTATCCAATCTTTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAGCACTTGGACCTTTCCAGCAACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTACATCAAATGCCCCTACTCAATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATCCAACCAGGTGCATTTAAAGAAATTAGGCTTCATAAGCTGACTTTAAGAAATAATTTTGATAGTTTAAATGTAATGAAAACTTGTATTCAAGGTCTGGCTGGTTTAGAAGTCCATCGTTTGGTTCTGGGAGAATTTAGAAATGAAGGAAACTTGGAAAAGTTTGACAAATCTGCTCTAGAGGGCCTGTGCAATTTGACCATTGAAGAATTCCGATTAGCATACTTAGACTACTACCTCGATGATATTATTGACTTATTTAATTGTTTGACAAATGTTTCTTCATTTTCCCTGGTGAGTGTGACTATTGAAAGGGTAAAAGACTTTTCTTATAATTTCGGATGGCAACATTTAGAATTAGTTAACTGTAAATTTGGACAGTTTCCCACATTGAAACTCAAATCTCTCAAAAGGCTTACTTTCACTTCCAACAAAGGTGGGAATGCTTTTTCAGAAGTTGATCTACCAAGCCTTGAGTTTCTAGATCTCAGTAGAAATGGCTTGAGTTTCAAAGGTTGCTGTTCTCAAAGTGATTTTGGGACAACCAGCCTAAAGTATTTAGATCTGAGCTTCAATGGTGTTATTACCATGAGTTCAAACTTCTTGGGCTTAGAACAACTAGAACATCTGGATTTCCAGCATTCCAATTTGAAACAAATGAGTGAGTTTTCAGTATTCCTATCACTCAGAAACCTCATTTACCTTGACATTTCTCATACTCACACCAGAGTTGCTTTCAATGGCATCTTCAATGGCTTGTCCAGTCTCGAAGTCTTGAAAATGGCTGGCAATTCTTTCCAGGAAAACTTCCTTCCAGATATCTTCACAGAGCTGAGAAACTTGACCTTCCTGGACCTCTCTCAGTGTCAACTGGAGCAGTTGTCTCCAACAGCATTTAACTCACTCTCCAGTCTTCAGGTACTAAATATGAGCCACAACAACTTCTTTTCATTGGATACGTTTCCTTATAAGTGTCTGAACTCCCTCCAGGTTCTTGATTACAGTCTCAATCACATAATGACTTCCAAAAAACAGGAACTACAGCATTTTCCAAGTAGTCTAGCTTTCTTAAATCTTACTCAGAATGACTTTGCTTGTACTTGTGAACACCAGAGTTTCCTGCAATGGATCAAGGACCAGAGGCAGCTCTTGGTGGAAGTTGAACGAATGGAATGTGCAACACCTTCAGATAAGCAGGGCATGCCTGTGCTGAGTTTGAATATCACCTGTCAGATGAATAAGACCATCATTGGTGTGTCGGTCCTCAGTGTGCTTGTAGTATCTGTTGTAGCAGTTCTGGTCTATAAGTTCTATTTTCACCTGATGCTTCTTGCTGGCTGCATAAAGTATGGTAGAGGTGAAAACATCTATGATGCCTTTGTTATCTACTCAAGCCAGGATGAGGACTGGGTAAGGAATGAGCTAGTAAAGAATTTAGAAGAAGGGGTGCCTCCATTTCAGCTCTGCCTTCACTACAGAGACTTTATTCCCGGTGTGGCCATTGCTGCCAACATCATCCATGAAGGTTTCCATAAAAGCCGAAAGGTGATTGTTGTGGTGTCCCAGCACTTCATCCAGAGCCGCTGGTGTATCTTTGAATATGAGATTGCTCAGACCTGGCAGTTTCTGAGCAGTCGTGCTGGTATCATCTTCATTGTCCTGCAGAAGGTGGAGAAGACCCTGCTCAGGCAGCAGGTGGAGCTGTACCGCCTTCTCAGCAGGAACACTTACCTGGAGTGGGAGGACAGTGTCCTGGGGCGGCACATCTTCTGGAGACGACTCAGAAAAGCCCTGCTGGATGGTAAATCATGGAATCCAGAAGGAACAGTGGGTACAGGATGCAATTGGCAGGAAGCAACATCTATCTGAAGAGGAAAAATAAAAACCTCCTGAGGCATTTCTTGCCCAGCTGGGTCCAACACTTGTTCAGTTAATAAGTATTAAATGCTGCCACATGTCAGGCCTTATGCTAAGGGTGAGTAATTCCATGGTGCACTAGATATGCAGGGCTGCTAATCTCAAGGAGCTTCCAGTGCAGAGGGAATAAATGCTAGACTAAAATACAGAGTCTTCCAGGTGGGCATTTCAACCAACTCAGTCAAGGAACCCATGACAAAGAAAGTCATTTCAACTCTTACCTCATCAAGTTGAATAAAGACAGAGAAAACAGAAAGAGACATTGTTCTTTTCCTGAGTCTTTTGAATGGAAATTGTATTATGTTATAGCCATCATAAAACCATTTTGGTAGTTTTGACTGAACTGGGTGTTCACTTTTTCCTTTTTGATTGAATACAATTTAAATTCTACTTGATGACTGCAGTCGTCAAGGGGCTCCTGATGCAAGATGCCCCTTCCATTTTAAGTCTGTCTCCTTACAGAGGTTAAAGTCTAGTGGCTAATTCCTAAGGAAACCTGATTAACACATGCTCACAACCATCCTGGTCATTCTCGAGCATGTTCTATTTTTTAACTAATCACCCCTGATATATTTTTATTTTTATATATCCAGTTTTCATTTTTTTACGTCTTGCCTATAAGCTAATATCATAAATAAGGTTGTTTAAGACGTGCTTCAAATATCCATATTAACCACTATTTTTCAAGGAAGTATGGAAAAGTACACTCTGTCACTTTGTCACTCGATGTCATTCCAAAGTTATTGCCTACTAAGTAATGACTGTCATGAAAGCAGCATTGAAATAATTTGTTTAAAGGGGGCACTCTTTTAAACGGGAAGAAAATTTCCGCTTCCTGGTCTTATCATGGACAATTTGGGCTAGAGGCAGGAAGGAAGTGGGATGACCTCAGGAGGTCACCTTTTCTTGATTCCAGAAACATATGGGCTGATAAACCCGGGGTGACCTCATGAAATGAGTTGCAGCAGAAGTTTATTTTTTTCAGAACAAGTGATGTTTGATGGACCTCTGAATCTCTTTAGGGAGACACAGATGGCTGGGATCCCTCCCCTGTACCCTTCTCACTGCCAGGAGAACTACGTGTGAAGGTATTCAAGGCAGGGAGTATACATTGCTGTTTCCTGTTGGGCAATGCTCCTTGACCACATTTTGGGAAGAGTGGATGTTATCATTGAGAAAACAATGTGTCTGGAATTAATGGGGTTCTTATAAAGAAGGTTCCCAGAAAAGAATGTTCATCCAGCCTCCTCAGAAACAGAACATTCAAGAAAAGGACAATCAGGATGTCATCAGGGAAATGAAAATAAAAACCACAATGAGATATCACCTTATACCAGGTAGAATGGCTACTATAAAAAAATGAAGTGTCATCAAGGATATAGAGAAATTGGAACCCTTCTTCACTGCTGGAGGGAATGGAAAATGGTGTAGCCGTTATGAAAAACAGTACGGAGGTTTCTCAAAAATTAAAAATAGAACTGCTATATGATCCAGCAATCTCACTTCTGTATATATACCCAAAATAATTGAAATCAGAATTTCAAGAAAATATTTACACTCCCATGTTCATTGTGGCACTCTTCACAATCACTGTTTCCAAAGTTATGGAAACAACCCAAATTTCCATTGAAAAATAAATGGACAAAGAAAATGTGCATATACGTACAATGGGATATTATTCAGCCTAAAAAAAGGGGGAATCCTGTTATTTATGACAACATGAATAAACCCGGAGGCCATTATGCTATGTAAAATGAGCAAGTAACAGAAAGACAAATACTGCCTGATTTCATTTATATGAGGTTCTAAAATAGTCAAACTCATAGAAGCAGAGAATAGAACAGTGGTTCCTAGGGAAAAGGAGGAAGGGAGAAATGAGGAAATAGGGAGTTGTCTAATTGGTATAAAATTATAGTATGCAAGATGAATTAGCTCTAAAGATCAGCTGTATAGCAGAGTTCGTATAATGAACAATACTGTATTATGCACTTAACATTTTGTTAAGAGGGTACCTCTCATGTTAAGTGTTCTTACCATATACATATACACAAGGAAGCTTTTGGAGGTGATGGATATATTTATTACCTTGATTGTGGTGATGGTTTGACAGGTATGTGACTATGTCTAAACTCATCAAATTGTATACATTAAATATATGCAGTTTTATAATATCAATTATGTCTGAATGAAGCTATAAAAAAGAAAAGACAACAAAATTCAGTTGTCAAAACTGGAAATATGACCACAGTCAGAAGTGTTTGTTACTGAGTGTTTCAGAGTGTGTTTGGTTTGAGCAGGTCTAGGGTGATTGAACATCCCTGGGTGTGTTTCCATGTCTCATGTACTAGTGAAAGTAGATGTGTGCATTTGTGCACATATCCCTATGTATCCCTATCAGGGCTGTGTGTATTTGAAAGTGTGTGTGTCCGCATGATCATATCTGTATAGAAGAGAGTGTGATTATATTTCTTGAAGAATACATCCATTTGAAATGGATGTCTATGGCTGTTTGAGATGAGTTCTCTACTCTTGTGCTTGTACAGTAGTCTCCCCTTATCCCTTATGCTTGGTGGATACGTTCTTAGACCCCAAGTGGATCTCTGAGACCGCAGATGGTACCAAACCTCATATATGCAATATTTTTTCCTATACATAAATACCTAAGATAAAGTTCATCTTCTGAATTAGGCACAGTAAGAGATTAACAATAACTAACAATAAAATTGAATAGTTATAATAATATATTGTAATAAAAGTTATGTGAATGTGATCTCTTTCTTTCTCTCTCTCAAAAT hsa-mir-216a 5p human microrna 216a 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UAAUCUCAGCUGGCAACUGUGA hsa_mir_216a 5p-1 2 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered physical association physical association Fig 5 A, B There is an evident mistake in the name of the mir in fig 5A. Moreover even if the seed sequence of the mRNA is in the refseq, its flanking region is different, so the right mutation site is probably only in the longest isoform of more than 12000 nucleotides. down-regulates quantity by destabilization hsa_mir_216a 5p-mrna_tlr4-1 1 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered physical association physical association Fig 5 A, B There is an evident mistake in the name of the mir in fig 5A. Moreover even if the seed sequence of the mRNA is in the refseq, its flanking region is different, so the right mutation site is probably only in the longest isoform of more than 12000 nucleotides. down-regulates quantity by destabilization