IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference confocal microscopy confocal microscopy immunostaining immunostaining Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 2018 imex curation Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2018/lazo-2017. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Accepted 2018-JAN-23 AT 09:38 GMT AT 09:38 GMT by MGT Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation Results from 7b added.; Mouse protein corrected, exp preps are fine.; All done. aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA DIP protein Q96GD4 original sequence version: 88 DIP protein Q96GD4 original sequence version: 113 DIP protein Q96GD4 original sequence version: 116 DIP protein Q96GD4 original sequence version: 122 DIP protein Q96GD4 original sequence version: 177 DIP protein Q96GD4-1 original sequence version: 177 A5ED13EF5A1FAFBF p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 h31t_human Histone H3.1t H3-4 H3/g H3FT HIST3H3 Histone H3.4 protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLMREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCVIHAKRVTIMPKDIQLARRIRGERA DIP protein Q16695 original sequence version: 117 37375A2C3A377B2B vrk1_human Serine/threonine-protein kinase VRK1 Vaccinia-related kinase 1 VRK1 protein protein human Homo sapiens Human MPRVKAAQAGRQSSAKRHLAEQFAVGEIITDMAKKEWKVGLPIGQGGFGCIYLADMNSSESVGSDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKEYKEDPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRKRVQK 5640C624BF059949 aurkb-vrk1-1 2 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 12 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:39:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 16:01:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' ecoli Escherichia coli (strain K12) physical association physical association 1a In a VRK1 pull-down (Fig. 1a) [...] AURKB was always brought down by VRK1, and the interaction was stronger with kinase-dead VRK1 (K179E). aurkb-vrk1-2 8 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed engineered engineered n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:41:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 18:24:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' physical association physical association 1b In [...] reciprocal immunoprecipitations (Fig. 1b, c), AURKB was always brought down by VRK1, and the interaction was stronger with kinase-dead VRK1 (K179E). In the immunoprecipitation of tagged AURKB, its interaction was also stronger with kinase-dead VRK1 (Fig. 1b, c). vrk1-aurkb-1 8 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed engineered engineered n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 19:50:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 18:39:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' physical association physical association 1c In [...] reciprocal immunoprecipitations (Fig. 1b, c), AURKB was always brought down by VRK1, and the interaction was stronger with kinase-dead VRK1 (K179E). In the immunoprecipitation of tagged AURKB, its interaction was also stronger with kinase-dead VRK1 (Fig. 1b, c). aurkb-vrk1-3 7 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 1d, 1e, 1f Interaction detected also in serum-deprived cells, during mitosis and faintly in asynchronous phase. Next, we determined if the VRK1–AURKB interaction was also detectable with endogenous proteins using extracts from HeLa cells. This interaction was also detected when VRK1, a very abundant nuclear protein [30], was immunoprecipitated, but not when the minority protein, AURKB was immunoprecipitated (Fig. 1d). The interaction between VRK1 and AURKB was mainly detected in mitotic cells (Fig. 1f) [...]. aurkb-vrk1-4 3 9 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 12 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization 2, 7d Only a minor colocalization of VRK1 and AURKB is detectable in anaphase in the central spindle. Only a minor colocalization of VRK1 and AURKB is detectable in anaphase in the central spindle. Nocodazole (CHEBI:34892) aurkb-vrk1-5 1 9 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 12 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization S2 The interaction is only detected after treatment with thymidine + nocodazole, which arrests cell cycle at early mitosis/G2 phase. aurkb-hist3h3-1 4 9 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96GD4:p.Lys106Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:49:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys106arg' Tue Jun 30 19:02:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys106Arg' ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). phosphorylation phosphorylation reaction phosphorylation 3a [...] phosphorylation of histone H3 by AURKB was inhibited by kinase-dead VRK1 (K179E) (Fig. 3a). vrk1-10 4 12 enzyme enzyme putative self putative self engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 18:38:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 19:20:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true 3a, S3a Autophosphorylation of VRK1 was inhibited by kinase-dead AURKB (Fig. 3a) [...]. vrk1-11 6 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). 12 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3c, 3d, S4 [...] the phosphorylation of histone H3 in Thr3 by VRK1 was inhibited in a dose-dependent manner by increasing amounts of AURKB (Fig. 3c, left) or kinase-dead AURKB (K106R) (Fig. 3c, right). The specific phosphorylation of Histone H3 in Thr3 or Ser10 by VRK1 or AURKB, respectively, was confirmed by in vitro kinase assays with purified proteins (Fig. 3d). hist3h3-vrk1-1 6 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). 9 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3b, 3d,S4 The specific phosphorylation of histone H3 in Ser10 by AURKB was inhibited in a dose-dependent manner by both VRK1 (Fig. 3b, left) and its kinase-dead form (Fig. 3b, right). hist3h3-vrk1-2 6 10 enzyme target enzyme target substrate substrate neutral component neutral component engineered engineered purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 12 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3e Furthermore, the specific phosphorylation of p53 in Thr18 by VRK1 [31–33] is also inhibited in a dose-dependent manner by kinase-dead AURKB (Fig. 3e). tp53-vrk1-2 4 10 enzyme target enzyme target substrate substrate neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 12 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation S3a aurkb-vrk1-6 5 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 4b Maximum strengh of this interaction was depected 360 minutes after nocodazole release. [...] the interaction between AURKB and VRK1 reached its maximum as demonstrated by immunoprecipitation (Fig. 4b). The increased detection of the AURKB–VRK1 complex (Fig. 4b) by immunoprecipitation [...] aurkb-vrk1-7 5 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain physical association physical association 7b [...] AURKB interaction with H3 (Fig. 7b) [...] VRK1 (Q99986), nocodazole (CHEBI:34892) Phosphorylation of Thr4 and Ser11 in H3 decreased in absence of VRK1. aurkb-hist3h3-2 4 12 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation mutation certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:56:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 20:24:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' ecoli Escherichia coli (strain K12) 9 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96GD4:p.Lys106Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:57:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys106arg' Tue Jun 30 19:33:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys106Arg' ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). phosphorylation phosphorylation reaction phosphorylation 3a, S3b [...] phosphorylation of histone H3 by AURKB was inhibited by kinase-dead VRK1 (K179E) (Fig. 3a). vrk1-12 4 9 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96GD4:p.Lys106Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Mon Jan 22 16:42:08 GMT 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Wed May 02 17:44:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys106arg' Tue Jun 30 17:11:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys106Arg' ecoli Escherichia coli (strain K12) 12 enzyme enzyme putative self putative self engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 17:38:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 19:55:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3a Autophosphorylation of VRK1 was inhibited by kinase-dead AURKB (Fig. 3a) [...]. aurkb-hist3h3-3 6 12 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99986:p.Lys179Glu mutation mutation certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:40:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179glu' Tue Jun 30 15:50:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Glu' ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). 9 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3b, S4 The specific phosphorylation of histone H3 in Ser10 by AURKB was inhibited in a dose-dependent manner by both VRK1 (Fig. 3b, left) and its kinase-dead form (Fig. 3b, right). tp53-vrk1-4 6 9 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96GD4:p.Lys106Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:02:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys106arg' Tue Jun 30 16:37:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys106Arg' ecoli Escherichia coli (strain K12) 10 enzyme target enzyme target substrate substrate neutral component neutral component engineered engineered purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 12 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3e Furthermore, the specific phosphorylation of p53 in Thr18 by VRK1 [31–33] is also inhibited in a dose-dependent manner by kinase-dead AURKB (Fig. 3e). hist3h3-vrk1-3 6 9 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96GD4:p.Lys106Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed May 02 17:53:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys106arg' Tue Jun 30 18:48:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys106Arg' ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). 12 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3c, S4 [...] the phosphorylation of histone H3 in Thr3 by VRK1 was inhibited in a dose-dependent manner by increasing amounts of AURKB (Fig. 3c, left) or kinase-dead AURKB (K106R) (Fig. 3c, right). The specific phosphorylation of Histone H3 in Thr3 or Ser10 by VRK1 or AURKB, respectively, was confirmed by in vitro kinase assays with purified proteins (Fig. 3d). aurkb-hist3h3-4 6 12 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). 9 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3bi The specific phosphorylation of histone H3 in Ser10 by AURKB was inhibited in a dose-dependent manner by both VRK1 (Fig. 3b, left) and its kinase-dead form (Fig. 3b, right). There is an inverse correlation between H3–S11 phosphorylation and H3–T4 phosphorylation as the ratio of AURKB/VRK1 changed. There is an inverse correlation between H3–S10ph and H3–T3ph as the ratio of AURKB/VRK1 changed. aurkb-hist3h3-5 6 9 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). 12 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 3c [...] the phosphorylation of histone H3 in Thr3 by VRK1 was inhibited in a dose-dependent manner by increasing amounts of AURKB (Fig. 3c, left) or kinase-dead AURKB (K106R) (Fig. 3c, right). The specific phosphorylation of Histone H3 in Thr3 or Ser10 by VRK1 or AURKB, respectively, was confirmed by in vitro kinase assays with purified proteins (Fig. 3d).