DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein. Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein. Nature 2017 imex curation Zhang Y., Sun B., Feng D., Hu H., Chu M., Qu Q., Tarrasch JT., Li S., Sun Kobilka T., Kobilka BK., Skiniotis G. skinioti@umich.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column structure determination identification by structure determination identification by structure determination structure determination Zhang Y., Sun B., Feng D., Hu H., Chu M., Qu Q., Tarrasch JT., Li S., Sun Kobilka T., Kobilka BK., Skiniotis G. skinioti@umich.edu Accepted 2018-JUL-25 AT 11:48 BST AT 11:48 BST by ORCHARD Nature 2017 imex curation Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein. Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein. Nature 2017 imex curation Zhang Y., Sun B., Feng D., Hu H., Chu M., Qu Q., Tarrasch JT., Li S., Sun Kobilka T., Kobilka BK., Skiniotis G. skinioti@umich.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Zhang Y., Sun B., Feng D., Hu H., Chu M., Qu Q., Tarrasch JT., Li S., Sun Kobilka T., Kobilka BK., Skiniotis G. skinioti@umich.edu Accepted 2018-JUL-25 AT 11:48 BST AT 11:48 BST by ORCHARD Nature 2017 imex curation p63092-2 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short GSP Alpha-S-short Alpha-S1 GNASs GNAS Adenylate cyclase-stimulating G alpha protein GNAS1 protein protein human Homo sapiens Human MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL mint C3D8B1E786EBC618 g1sgd4_rabit GLP1R protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MAGAPSPLRLALLLLGAVGRAGPRPQGATVSLSETVQKWREYRRQCQHFLTEAPPLATGLFCNRTFDDYACWPDGAPGSFVNVSCPWYLPWASNVLQGHVYRFCTTEGHWLHKDNSSLPWRDLSECEESRRGEKSSPEEQLLSLYIIYTVGYALSFSALVIASAILLGFRHLHCTRNYIHLNLFASFILRALSVFIKDAALKWMYSTAAQQHQWDGLLSYQDSLGCRLVFLLMQYCVAANYYWLLVEGAYLYTLLAFAVFSEQRIFKLYLSIGWGVPLLFVIPWGIVKYLYEDEGCWTRNSNMNYWLIIRLPILFAIGVNFLIFIRVICIVVSKLKANLMCKTDIKCRLAKSTLTLIPLLGTHEVIFAFVMDEHARGTLRFVKLFTELSFTSFQGLMVAILYCFVNNEVQMEFRKSWERWRLEHLRVQRDSSMKPLKCPTSSLSSGATGGSSVYAATSQACS 2F578AC6ECF78A9F p01275-pro_0000011259 Glucagon-like peptide 1(7-37) GCG protein protein human Homo sapiens Human HAEGTFTSDVSSYLEGQAAKEFIAWLVKGRG 98 128 22BE1C586B4D6E52 gbg2_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 GNG2 G gamma-I protein protein bovin Bos taurus (Bovine) Bovine MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL DIP protein P63212 original sequence version: 54 DIP protein P63212 original sequence version: 55 DIP protein P63212 original sequence version: 66 EDB74E4135E7A37A gbb1_rat Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Transducin beta chain 1 Gnb1 protein protein rat Rattus norvegicus (Rat) Rat MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN DIP protein P54311 original sequence version: 123 DIP protein P54311 original sequence version: 96 896CBD32D2686598 as-2 2 3 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 6 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association Fig 1 gcg-glp1r-1 1 3 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association Extended Data Fig 1