IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation. PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation. The Journal of experimental medicine 2016 imex curation Kong D., Shen Y., Liu G., Zuo S., Ji Y., Lu A., Nakamura M., Lazarus M., Stratakis CA., Breyer RM., Yu Y. 20/07/2018: Sent email to authors for info on the cell line you used in the Coimmunoprecipitation experiment shown in Fig. 7K and on the origin species of the following protein: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7) INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8) PRKAR2A in the construct HA-PRKAR2A(fig.7) PRKAR2A in the construct His-PRKAR2A(fig.8). NO REPLY Interactions depicted in figure 7K were not included because of the lack of information about the host cell line. Interactions involving the following constructs were not included because of lack of information about species of origin: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7), INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8), PRKAR2A in the construct HA-PRKAR2A(fig.7) and PRKAR2A in the construct His-PRKAR2A(fig.8). The authors were contacted by email requesting additional information but did not reply. yuying@sibs.ac.cn, yuying@tmu.edu.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-macrophage Mus musculus macrophage macrophage Macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kong D., Shen Y., Liu G., Zuo S., Ji Y., Lu A., Nakamura M., Lazarus M., Stratakis CA., Breyer RM., Yu Y. 20/07/2018: Sent email to authors for info on the cell line you used in the Coimmunoprecipitation experiment shown in Fig. 7K and on the origin species of the following protein: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7) INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8) PRKAR2A in the construct HA-PRKAR2A(fig.7) PRKAR2A in the construct His-PRKAR2A(fig.8). NO REPLY Interactions depicted in figure 7K were not included because of the lack of information about the host cell line. Interactions involving the following constructs were not included because of lack of information about species of origin: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7), INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8), PRKAR2A in the construct HA-PRKAR2A(fig.7) and PRKAR2A in the construct His-PRKAR2A(fig.8). The authors were contacted by email requesting additional information but did not reply. yuying@sibs.ac.cn, yuying@tmu.edu.cn Accepted 2018-OCT-09 AT 17:15 BST AT 17:15 BST by PPORRAS The Journal of experimental medicine 2016 Only protein-protein interactions imex curation imex curation PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation. PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation. The Journal of experimental medicine 2016 imex curation Kong D., Shen Y., Liu G., Zuo S., Ji Y., Lu A., Nakamura M., Lazarus M., Stratakis CA., Breyer RM., Yu Y. 20/07/2018: Sent email to authors for info on the cell line you used in the Coimmunoprecipitation experiment shown in Fig. 7K and on the origin species of the following protein: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7) INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8) PRKAR2A in the construct HA-PRKAR2A(fig.7) PRKAR2A in the construct His-PRKAR2A(fig.8). NO REPLY Interactions depicted in figure 7K were not included because of the lack of information about the host cell line. Interactions involving the following constructs were not included because of lack of information about species of origin: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7), INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8), PRKAR2A in the construct HA-PRKAR2A(fig.7) and PRKAR2A in the construct His-PRKAR2A(fig.8). The authors were contacted by email requesting additional information but did not reply. yuying@sibs.ac.cn, yuying@tmu.edu.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-macrophage Mus musculus macrophage macrophage Macrophage confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry Kong D., Shen Y., Liu G., Zuo S., Ji Y., Lu A., Nakamura M., Lazarus M., Stratakis CA., Breyer RM., Yu Y. 20/07/2018: Sent email to authors for info on the cell line you used in the Coimmunoprecipitation experiment shown in Fig. 7K and on the origin species of the following protein: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7) INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8) PRKAR2A in the construct HA-PRKAR2A(fig.7) PRKAR2A in the construct His-PRKAR2A(fig.8). NO REPLY Interactions depicted in figure 7K were not included because of the lack of information about the host cell line. Interactions involving the following constructs were not included because of lack of information about species of origin: INF-gammaR2 in the construct FLAG-INF-gammaR2 (fig.7), INF-gammaR2 in the construct GST-INF-gammaR2 (fig. 8), PRKAR2A in the construct HA-PRKAR2A(fig.7) and PRKAR2A in the construct His-PRKAR2A(fig.8). The authors were contacted by email requesting additional information but did not reply. yuying@sibs.ac.cn, yuying@tmu.edu.cn Accepted 2018-OCT-09 AT 17:15 BST AT 17:15 BST by PPORRAS The Journal of experimental medicine 2016 Only protein-protein interactions imex curation imex curation ingr2_human Interferon gamma receptor 2 Interferon gamma receptor beta-chain IFNGR2 IFNGT1 Interferon gamma receptor accessory factor 1 Interferon gamma transducer 1 protein protein human Homo sapiens Human MRPTLLWSLLLLLGVFAAAAAAPPDPLSQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYQVQFKYTDSKWFTADIMSIGVNCTQITATECDFTAASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNISCYETMADASTELQQVILISVGTFSLLSVLAGACFFLVLKYRGLIKYWFHTPPSIPLQIEEYLKDPTQPILEALDKDSSPKDDVWDSVSIISFPEKEQEDVLQTL 18C68BAF7D91B8AA kap2_human cAMP-dependent protein kinase type II-alpha regulatory subunit PKR2 PRKAR2 PRKAR2A protein protein human Homo sapiens Human MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ DIP protein P13861 original sequence version: 107 DIP protein P13861 original sequence version: 128 C7D6E8E476E1DB65 ifngr2-prkar2a-1 1 4 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 7R, Fig. 7S CHEBI:131496 - BW 245C Consistent with these observations, BW augmented the PRKAR2A–IFN-γR2 interaction (Fig. 7 R), which was blunted by Rp-cAMP in a dose-dependent manner (Fig. 7 S). CHEBI:84622 - (Rp)-cAMPS ifngr2-prkar2a-2 2 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 3 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization Fig. 7P, Fig. 7Q DP1 activation increased the membrane mobilization of free PRKAR2A in macrophages, as well as its colocalization with IFN-γR2, whereas this was markedly decreased in Mac-DP1 KO macrophages CHEBI:131496 - BW 245C