IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Cardiovascular research 2014 imex curation Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. qjing@sibs.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 ch-ip chromatin immunoprecipitation assay ch-ip qrt-pcr quantitative reverse transcription pcr QRT-PCR Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. Cells were treated with oxidized low density lipoprotein. qjing@sibs.ac.cn Accepted 2018-MAY-29 AT 15:44 BST AT 15:44 BST by MGT Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Cardiovascular research 2014 imex curation Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. qjing@sibs.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift tag visualisation tag visualisation Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. qjing@sibs.ac.cn Accepted 2018-MAY-29 AT 15:44 BST AT 15:44 BST by MGT Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Cardiovascular research 2014 imex curation Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. qjing@sibs.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. qjing@sibs.ac.cn Accepted 2018-MAY-29 AT 15:44 BST AT 15:44 BST by MGT Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis. Cardiovascular research 2014 imex curation Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. qjing@sibs.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Tian FJ, Tian FJ., An LN., Wang GK., Zhu JQ., Li Q., Zhang YY., Zeng A., Zou J., Zhu RF., Han XS., Shen N., Yang HT., Zhao XX., Huang S., Qin YW., Jing Q. Cells were treated with oxidized low density lipoprotein. qjing@sibs.ac.cn Accepted 2018-MAY-29 AT 15:44 BST AT 15:44 BST by MGT Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation mrna_hbp1 HMG-box transcription factor 1 mRNA mrna messenger rna mRNA mrna mouse Mus musculus Mouse GGGGAGGGGGAAGACGAAGCTTGAAAGACTTGGTAATGGCGACGGGTTTGTCGGAGCACCATAACATGGTGTGGGAAGTGAAGACAAATCAGATGCCTAATGCAGTACAGAAACTCCTGCTGGTGATGGACAAGAGAGCACCAGGGATGAGTGACTCGCTGGAGTTGCTGCAGTGTAATGAGAATTTGCCATCCTCTCCAGGATACAACTCCTGTGATGAGCACATGGAGCTTGATGACCTTCCTGAACTTCAGGCTGTTCAAAGTGACCCTACCCAATCTGCCATATACCAGCTAAGTTCAGATGTATCTCATCAAGAATATCCAAGATCATCTTGGAGCCAGAATACCTCAGATATACCGGAAAATACTCACCGTGAAGATGAGGTGGACTGGCTAACAGAGTTAGCAAATATTGCCACTAGTCCACAAAGTCCCCTGATGCAGTGCTCATTTTATAACAGATCATCTCCTGTACACATCATAGCTACTAGCAAAAGTTTACATTCCTATGCACGCCCACCACCAGTGTCCTCTTCTTCAAAAAGTGGGCCAGCCTTCCCTCATGACCACTGGAAGGAAGAAACACCAGTAAGACATGAAAGGGCAAACAGTGAGTCAGAGTCAGGCATTTTCTGTATGTCCTCCCTGTCAGATGATGATGATCTGGGCTGGTGCAATTCCTGGCCATCCACCATTTGGCACTGCTTTCTGAAAGGCACGCGGCTGTGCTTCCATAAGGAAAGCAATAAGGAATGGCAAGATGTGGAAGACTTTGCTAGAGCCGCCAGCTGTGATAATGAGGAAGAGATCCAAATGGGCACTCACAAGGGCTATGGGTCTGATGGTCTAAAGTTGTTATCCCATGAAGAGAGTGTGTCATTCGGTGAGTCTGTACTGAAGTTGACATTTGATCCTGGTACAGTAGAAGATGGCTTGCTCACTGTAGAGTGCAAGCTGGACCACCCTTTCTATGTTAAAAATAAAGGTTGGTCGTCATTTTATCCAAGCTTGACTGTGGTACAGCATGGCATTCCATGTTGTGAAATTCATATTGGTGACGTATGTCTACCTCCTGGACACCCTGATGCCATTAATTTCGATGATTCAGGTGTTTTTGATACATTTAAAAGCTATGACTTCACACCTATGGACTCTTCTGCGGTCTATGTTTTGAGTAGTATGGCTCGTCAGCGCCGTGCATCTCTGTCTTGTGGAGGAGGACCTGGTACTGGTCAAGAGTTTTCGGGATCTGAATTCAGTAAAAGCTGTGGTTCACCTGGATCATCACAGCTCTCTTCTAGTTCTTTGTATGCTAAAGCTGTCAAAAGCCACAGCTCAGGGACTGTGAGTGCCACTTCTCCTAATAAGTGCAAAAGACCAATGAATGCCTTCATGCTTTTTGCCAAAAAATACAGAGTTGAATATACTCAGATGTATCCAGGGAAAGATAACAGAGCCATAAGTGTGATCCTTGGTGACAGGTGGAAAAAAATGAAGAACGAAGAAAGGAGGATGTACACATTAGAAGCAAAGGCTTTGGCTGAAGAACAAAAGCGTTTAAATCCTGACTGTTGGAAAAGGAAAAGAACCAACTCAGGCTCTCAACAGCATTAAACCTGATGCTTGTGTTTGTAAGTCTGCATCTGCATAAGTACTGACTCTGGATGGAAAAGCTTGGAAGACCTTGGTCTCACCATTTGTCTACATTTGTGTGACCATAAAATGCTGGGAGCATTGAGTTTTGTAATGATTTAATGGGTGAGGATTGCTTTCTCCATTAGAGCATTAAGCTATAACTATCAACATTGTAAACCAAATTGCCTTATTTTCCTTCCAAACTTCATATATCTATCAGGTAATATAGGCTTGAAAATTGATATCCTGTGGTGCTAAAGTACAGTAGAAAGATGTGTATACGTTTTATTTTAAATTGTACGAAGGGGAATTTAAAAATACGTAACTGCTGTTTATACATCAGCTCCTTACTGCTTATTAATCTGTATTGTACCCACGATATCAGGAGTGAAGCAGACGCTGGCCTTTCCATGAATAGCCACCACATGGCCCAGCATCTCTGCCAAACACAGTTGCTTCTAGTCTGGCCAGTGCCAAGAGACAACATAACATGGCAGGTGGATGGTGTCGGTGTCATTGTCTCAGCTCAAAGAGCCACCTGCTTCAGATACCATGGGCATGTGCTGAGTGGATGCACCAGGGGGCAGCACAGCTCCATGATTCCCAGTGGGGCCCAAGCCAGGCCAAGGTGCAGTCAGTTAATAGCATTGGGACAGTCATTGTAATGGTGCTGTTACCAGTCGACACCATTGTGTTTGACCGCGTTCCTGTCTCCTCAGCCACATACCCTCATCTGTGCTGTCAGTGTGTCTCTGGTGGGGCAGACTTTGCACTTACTGCAGTGCACCACTTGCACTTTACTCCCTCCGGCTTCTGAGACAGAGCAAGTACTTCAGCAGTCCTGCTGCTCTTGCTCTGAGCTACAATCATGGCTTCTCATGTTACTTACCAAGTGAGAGTTTTGGTTAGTAATCACAGCTGTAAAATTGATTTACACTTGTTAAACATATTGCCCCTCAATTTTGCACACTATATTCTTGTATATTATTTCAAATAAAAGGAAAAAAAAGGTCT mmu-mir-155 5p miR-155 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse UUAAUGCUAAUUGUGAUAGGGGU tyy1_mouse Transcriptional repressor protein YY1 Yy1 Ucrbp Delta transcription factor NF-E1 UCR-motif DNA-binding protein Yin and yang 1 protein protein mouse Mus musculus Mouse MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEEEDDDDEDGGGGDHGGGGGGHGHAGHHHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGASSGGGRVKKGGGKKSGKKSYLGGGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ DIP protein Q00899 original sequence version: 121 DIP protein Q00899 original sequence version: 107 DIP protein Q00899 original sequence version: 112 C012378288E984F9 gene_mir155 gene gene mouse Mus musculus Mouse site_c_probe_mir-155_promoter dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse CTCCTTCTGCACCCGCCATGTTCTTTTTATAAGGACATC mir_155-mrna_hbp1-1 3 5 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined r.1786_1790delinsggcct mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain CATTA GGCCT human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line 6 regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line physical association physical association Fig 2C, Suppl Fig 5 yy1-gene_mir155-1 4 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level site_C_promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 antibody detection identification by antibody antibody detection primer specific pcr primer specific pcr unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association Suppl. Fig 9D YY1 binds directly to the promoter region of pri-miR-155 in oxLDL-induced macrophages yy1-gene_mir155-2 1 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level site_C_promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 antibody detection identification by antibody antibody detection qrt-pcr quantitative reverse transcription pcr QRT-PCR unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4C YY1 binds directly to the promoter region of pri-miR-155 in oxLDL-induced macrophages yy1-gene_mir155-3 2 7 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 9 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined synthetic construct synthetic construct physical association physical association Fig 4B YY1 binds directly to the promoter region of pri-miR-155 in oxLDL-induced macrophages