IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Isolated Toll-like receptor transmembrane domains are capable of oligomerization. Isolated Toll-like receptor transmembrane domains are capable of oligomerization. PloS one 2011 imex curation Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. By varying detergent concentration an apparent Kd can be determined from self-quenching interactions of TMD peptides with a fluorescent tag;at low detergent:peptide ratios, the TMD peptides interact; however, increasing the amount of detergent comcentration will cause the TMDs to dissociate, and fluorescence plateaus . hubert.yin@colorado.edu Accepted 2018-APR-12 AT 07:19 BST AT 07:19 BST by LIVIAP PloS one 2011 Only protein-protein interactions imex curation imex curation For Protein Complementation Assays Fusion tag are not added as from Manual:- "Tags need not be added if they are present in the Interaction Detection Method. " -which they are for this method. No - I did mean Table 1 -this gives the sequences of the TM domains, which are not given elsewhere. Table S1/2 is some statistical treatment similar to a T test but comparing different pairs of means and see which are significantly different from each other.- so not relevant I think. ; Basically I do not believe them; the stated average M.Wt they found is 14.1kDa. The M.Wt of the peptide + fluorescein is (2.1 kDa +~~3.3 kDa) = ~5.4 kDa for a monomer. So that might mean it is a trimer! I do not see how this becomes a "monomer-dimer-tetramer equilibrium". -there could be other explanations, so if in doubt we are not meant to enter data. (we can ask Pablo if you like) Isolated Toll-like receptor transmembrane domains are capable of oligomerization. Isolated Toll-like receptor transmembrane domains are capable of oligomerization. PloS one 2011 imex curation Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) toxcat tox-r dimerization assay toxcat predetermined predetermined participant predetermined Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. this assay system is not quite a match for "toxcat" - so could be a "protein complementation assay"? - but match for Toxcat is better than some... In the public Database:The "roles" and "interaction type" are all over the place- so have entered as Physical assoc and roles as Neutral - no idea what should be used. ToxR reporter assay:a chimeric construct ToxR-TM-MBP composed of the DNA binding/transcriptional activation domain of ToxR (a dimerization dependant transcription factor) is fused to a transmembrane domain of various TLRs and a monomeric periplasmic anchor (the maltose binding protein). Association of two TMs induces expression of a reporter enzyme, in this instance, beta-galactosidase. hubert.yin@colorado.edu Accepted 2018-APR-12 AT 07:19 BST AT 07:19 BST by LIVIAP PloS one 2011 Only protein-protein interactions imex curation imex curation For Protein Complementation Assays Fusion tag are not added as from Manual:- "Tags need not be added if they are present in the Interaction Detection Method. " -which they are for this method. No - I did mean Table 1 -this gives the sequences of the TM domains, which are not given elsewhere. Table S1/2 is some statistical treatment similar to a T test but comparing different pairs of means and see which are significantly different from each other.- so not relevant I think. ; Basically I do not believe them; the stated average M.Wt they found is 14.1kDa. The M.Wt of the peptide + fluorescein is (2.1 kDa +~~3.3 kDa) = ~5.4 kDa for a monomer. So that might mean it is a trimer! I do not see how this becomes a "monomer-dimer-tetramer equilibrium". -there could be other explanations, so if in doubt we are not meant to enter data. (we can ask Pablo if you like) Isolated Toll-like receptor transmembrane domains are capable of oligomerization. Isolated Toll-like receptor transmembrane domains are capable of oligomerization. PloS one 2011 imex curation Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu Accepted 2018-APR-12 AT 07:19 BST AT 07:19 BST by LIVIAP PloS one 2011 Only protein-protein interactions imex curation imex curation For Protein Complementation Assays Fusion tag are not added as from Manual:- "Tags need not be added if they are present in the Interaction Detection Method. " -which they are for this method. No - I did mean Table 1 -this gives the sequences of the TM domains, which are not given elsewhere. Table S1/2 is some statistical treatment similar to a T test but comparing different pairs of means and see which are significantly different from each other.- so not relevant I think. ; Basically I do not believe them; the stated average M.Wt they found is 14.1kDa. The M.Wt of the peptide + fluorescein is (2.1 kDa +~~3.3 kDa) = ~5.4 kDa for a monomer. So that might mean it is a trimer! I do not see how this becomes a "monomer-dimer-tetramer equilibrium". -there could be other explanations, so if in doubt we are not meant to enter data. (we can ask Pablo if you like) Isolated Toll-like receptor transmembrane domains are capable of oligomerization. Isolated Toll-like receptor transmembrane domains are capable of oligomerization. PloS one 2011 imex curation Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu Accepted 2018-APR-12 AT 07:19 BST AT 07:19 BST by LIVIAP PloS one 2011 Only protein-protein interactions imex curation imex curation For Protein Complementation Assays Fusion tag are not added as from Manual:- "Tags need not be added if they are present in the Interaction Detection Method. " -which they are for this method. No - I did mean Table 1 -this gives the sequences of the TM domains, which are not given elsewhere. Table S1/2 is some statistical treatment similar to a T test but comparing different pairs of means and see which are significantly different from each other.- so not relevant I think. ; Basically I do not believe them; the stated average M.Wt they found is 14.1kDa. The M.Wt of the peptide + fluorescein is (2.1 kDa +~~3.3 kDa) = ~5.4 kDa for a monomer. So that might mean it is a trimer! I do not see how this becomes a "monomer-dimer-tetramer equilibrium". -there could be other explanations, so if in doubt we are not meant to enter data. (we can ask Pablo if you like) Isolated Toll-like receptor transmembrane domains are capable of oligomerization. Isolated Toll-like receptor transmembrane domains are capable of oligomerization. PloS one 2011 imex curation Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. hubert.yin@colorado.edu 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) toxcat tox-r dimerization assay toxcat predetermined predetermined participant predetermined Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H., Godfroy JI., Roostan M., Moroz YS., Korendovych IV., Yin H. Fig 7B: I have not added any results for TLR2 with TLR4/5 as these are meant not to react -(much?)- from fig 3B . So what the mutations in fig 7B are meant to show? Modification of the ToxR reporter assay:a chimeric construct of either a)ToxR-TM-MBP composed of the DNA binding/transcriptional activation domain of a functional ToxR (a dimerization dependant transcription factor) OR b) a non-functional mutated ToxR*, is fused to a transmembrane domain of various TLRs and a monomeric periplasmic anchor (the maltose binding protein). Any interaction between the functional ToxR and non-functional ToxR* TMDs will result in a reduction in signal output as compared to the homotypic interaction. A ~50% reduction of signal indicates a positive interaction. As before, association of two TMs induces expression of a reporter enzyme, in this instance, beta-galactosidase. hubert.yin@colorado.edu Accepted 2018-APR-12 AT 07:19 BST AT 07:19 BST by LIVIAP PloS one 2011 Only protein-protein interactions imex curation imex curation For Protein Complementation Assays Fusion tag are not added as from Manual:- "Tags need not be added if they are present in the Interaction Detection Method. " -which they are for this method. No - I did mean Table 1 -this gives the sequences of the TM domains, which are not given elsewhere. Table S1/2 is some statistical treatment similar to a T test but comparing different pairs of means and see which are significantly different from each other.- so not relevant I think. ; Basically I do not believe them; the stated average M.Wt they found is 14.1kDa. The M.Wt of the peptide + fluorescein is (2.1 kDa +~~3.3 kDa) = ~5.4 kDa for a monomer. So that might mean it is a trimer! I do not see how this becomes a "monomer-dimer-tetramer equilibrium". -there could be other explanations, so if in doubt we are not meant to enter data. (we can ask Pablo if you like) lmp1_ebva8 Latent membrane protein 1 LMP1 Protein p63 BNLF1 protein protein ebva8 Epstein-Barr virus (strain AG876) (HHV-4) Human herpesvirus 4 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIVMSDWTGGALLVLYSFALILIIIILIIFIFRRDLLCPLGALCLLLLMITLLLIALWNLHGQALYLGIVLFIFGCLLVLGLWIYLLEILWRLGATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLLFLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSNSNEGRHHLLVTGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTDDNGPQDPDNTDDNGPQDPDNTDDNGPQDPDNTADNGPHDPLPHNPSDSAGNDGGPPNLTEEVENKGGDRGPPSMTDGGGGDPHLPTLLLGTSGSGGDDDDPHGPVQLSYYD E9DCE7BF5F174DC9 tlr10_human Toll-like receptor 10 TLR10 UNQ315/PRO358 protein protein human Homo sapiens Human MRLIRNIYIFCSIVMTAEGDAPELPEERELMTNCSNMSLRKVPADLTPATTTLDLSYNLLFQLQSSDFHSVSKLRVLILCHNRIQQLDLKTFEFNKELRYLDLSNNRLKSVTWYLLAGLRYLDLSFNDFDTMPICEEAGNMSHLEILGLSGAKIQKSDFQKIAHLHLNTVFLGFRTLPHYEEGSLPILNTTKLHIVLPMDTNFWVLLRDGIKTSKILEMTNIDGKSQFVSYEMQRNLSLENAKTSVLLLNKVDLLWDDLFLILQFVWHTSVEHFQIRNVTFGGKAYLDHNSFDYSNTVMRTIKLEHVHFRVFYIQQDKIYLLLTKMDIENLTISNAQMPHMLFPNYPTKFQYLNFANNILTDELFKRTIQLPHLKTLILNGNKLETLSLVSCFANNTPLEHLDLSQNLLQHKNDENCSWPETVVNMNLSYNKLSDSVFRCLPKSIQILDLNNNQIQTVPKETIHLMALRELNIAFNFLTDLPGCSHFSRLSVLNIEMNFILSPSLDFVQSCQEVKTLNAGRNPFRCTCELKNFIQLETYSEVMMVGWSDSYTCEYPLNLRGTRLKDVHLHELSCNTALLIVTIVVIMLVLGLAVAFCCLHFDLPWYLRMLGQCTQTWHRVRKTTQEQLKRNVRFHAFISYSEHDSLWVKNELIPNLEKEDGSILICLYESYFDPGKSISENIVSFIEKSYKSIFVLSPNFVQNEWCHYEFYFAHHNLFHENSDHIILILLEPIPFYCIPTRYHKLKALLEKKAYLEWPKDRRKCGLFWANLRAAINVNVLATREMYELQTFTELNEESRGSTISLMRTDCL 6BFAAEF76886BFBA tlr2_human Toll-like receptor 2 Toll/interleukin-1 receptor-like protein 4 TLR2 TIL4 protein protein human Homo sapiens Human MPHTLWMVWVLGVIISLSKEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDRVIDPGKVETLTIRRLHIPRFYLFYDLSTLYSLTERVKRITVENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACEDAWPSLQTLILRQNHLASLEKTGETLLTLKNLTNIDISKNSFHSMPETCQWPEKMKYLNLSSTRIHSVTGCIPKTLEILDVSNNNLNLFSLNLPQLKELYISRNKLMTLPDASLLPMLLVLKISRNAITTFSKEQLDSFHTLKTLEAGGNNFICSCEFLSFTQEQQALAKVLIDWPANYLCDSPSHVRGQQVQDVRLSVSECHRTALVSGMCCALFLLILLTGVLCHRFHGLWYMKMMWAWLQAKRKPRKAPSRNICYDAFVSYSERDAYWVENLMVQELENFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTVFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPMDEAQREGFWVNLRAAIKS DIP protein O60603 original sequence version: 92 DIP protein O60603 original sequence version: 114 7DBE6B24CF1FAF8B tlr4_human Toll-like receptor 4 TLR4 hToll protein protein human Homo sapiens Human MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI 92C48F55821133E8 tlr1_human Toll-like receptor 1 TLR1 KIAA0012 Toll/interleukin-1 receptor-like protein protein protein human Homo sapiens Human MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSKLRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQLKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSVKTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISNVKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPFLHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCSWTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFSSLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPESYRGTLLKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRARNIPLEELQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVENIITCIEKSYKSIFVLSPNFVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIPSSYHKLKSLMARRTYLEWPKEKSKRGLFWANLRAAINIKLTEQAKK 1BFCCC5E42EA5242 glpa_human Glycophorin-A PAS-2 Sialoglycoprotein alpha MN sialoglycoprotein GYPA GPA protein protein human Homo sapiens Human MYGKIIFVLLLSEIVSISASSTTGVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ 48A5450E22FA99C9 tlr6_human Toll-like receptor 6 TLR6 protein protein human Homo sapiens Human MTKDKEPIVKSFHFVCLMIIIVGTRIQFSDGNEFAVDKSKRGLIHVPKDLPLKTKVLDMSQNYIAELQVSDMSFLSELTVLRLSHNRIQLLDLSVFKFNQDLEYLDLSHNQLQKISCHPIVSFRHLDLSFNDFKALPICKEFGNLSQLNFLGLSAMKLQKLDLLPIAHLHLSYILLDLRNYYIKENETESLQILNAKTLHLVFHPTSLFAIQVNISVNTLGCLQLTNIKLNDDNCQVFIKFLSELTRGSTLLNFTLNHIETTWKCLVRVFQFLWPKPVEYLNIYNLTIIESIREEDFTYSKTTLKALTIEHITNQVFLFSQTALYTVFSEMNIMMLTISDTPFIHMLCPHAPSTFKFLNFTQNVFTDSIFEKCSTLVKLETLILQKNGLKDLFKVGLMTKDMPSLEILDVSWNSLESGRHKENCTWVESIVVLNLSSNMLTDSVFRCLPPRIKVLDLHSNKIKSVPKQVVKLEALQELNVAFNSLTDLPGCGSFSSLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELREFVKNIDQVSSEVLEGWPDSYKCDYPESYRGSPLKDFHMSELSCNITLLIVTIGATMLVLAVTVTSLCIYLDLPWYLRMVCQWTQTRRRARNIPLEELQRNLQFHAFISYSEHDSAWVKSELVPYLEKEDIQICLHERNFVPGKSIVENIINCIEKSYKSIFVLSPNFVQSEWCHYELYFAHHNLFHEGSNNLILILLEPIPQNSIPNKYHKLKALMTQRTYLQWPKEKSKRGLFWANIRAAFNMKLTLVTENNDVKS 9ACEAEC084CB466C tlr5_human Toll-like receptor 5 TLR5 TIL3 Toll/interleukin-1 receptor-like protein 3 protein protein human Homo sapiens Human MGDHLDLLLGVVLMAGPVFGIPSCSFDGRIAFYRFCNLTQVPQVLNTTERLLLSFNYIRTVTASSFPFLEQLQLLELGSQYTPLTIDKEAFRNLPNLRILDLGSSKIYFLHPDAFQGLFHLFELRLYFCGLSDAVLKDGYFRNLKALTRLDLSKNQIRSLYLHPSFGKLNSLKSIDFSSNQIFLVCEHELEPLQGKTLSFFSLAANSLYSRVSVDWGKCMNPFRNMVLEILDVSGNGWTVDITGNFSNAISKSQAFSLILAHHIMGAGFGFHNIKDPDQNTFAGLARSSVRHLDLSHGFVFSLNSRVFETLKDLKVLNLAYNKINKIADEAFYGLDNLQVLNLSYNLLGELYSSNFYGLPKVAYIDLQKNHIAIIQDQTFKFLEKLQTLDLRDNALTTIHFIPSIPDIFLSGNKLVTLPKINLTANLIHLSENRLENLDILYFLLRVPHLQILILNQNRFSSCSGDQTPSENPSLEQLFLGENMLQLAWETELCWDVFEGLSHLQVLYLNHNYLNSLPPGVFSHLTALRGLSLNSNRLTVLSHNDLPANLEILDISRNQLLAPNPDVFVSLSVLDITHNKFICECELSTFINWLNHTNVTIAGPPADIYCVYPDSFSGVSLFSLSTEGCDEEEVLKSLKFSLFIVCTVTLTLFLMTILTVTKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFSSKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDFQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATIS 9EE0AB6EEFEA9051 tlr7_human Toll-like receptor 7 TLR7 UNQ248/PRO285 protein protein human Homo sapiens Human MVFPMWTLKRQILILFNIILISKLLGARWFPKTLPCDVTLDVPKNHVIVDCTDKHLTEIPGGIPTNTTNLTLTINHIPDISPASFHRLDHLVEIDFRCNCVPIPLGSKNNMCIKRLQIKPRSFSGLTYLKSLYLDGNQLLEIPQGLPPSLQLLSLEANNIFSIRKENLTELANIEILYLGQNCYYRNPCYVSYSIEKDAFLNLTKLKVLSLKDNNVTAVPTVLPSTLTELYLYNNMIAKIQEDDFNNLNQLQILDLSGNCPRCYNAPFPCAPCKNNSPLQIPVNAFDALTELKVLRLHSNSLQHVPPRWFKNINKLQELDLSQNFLAKEIGDAKFLHFLPSLIQLDLSFNFELQVYRASMNLSQAFSSLKSLKILRIRGYVFKELKSFNLSPLHNLQNLEVLDLGTNFIKIANLSMFKQFKRLKVIDLSVNKISPSGDSSEVGFCSNARTSVESYEPQVLEQLHYFRYDKYARSCRFKNKEASFMSVNESCYKYGQTLDLSKNSIFFVKSSDFQHLSFLKCLNLSGNLISQTLNGSEFQPLAELRYLDFSNNRLDLLHSTAFEELHKLEVLDISSNSHYFQSEGITHMLNFTKNLKVLQKLMMNDNDISSSTSRTMESESLRTLEFRGNHLDVLWREGDNRYLQLFKNLLKLEELDISKNSLSFLPSGVFDGMPPNLKNLSLAKNGLKSFSWKKLQCLKNLETLDLSHNQLTTVPERLSNCSRSLKNLILKNNQIRSLTKYFLQDAFQLRYLDLSSNKIQMIQKTSFPENVLNNLKMLLLHHNRFLCTCDAVWFVWWVNHTEVTIPYLATDVTCVGPGAHKGQSVISLDLYTCELDLTNLILFSLSISVSLFLMVMMTASHLYFWDVWYIYHFCKAKIKGYQRLISPDCCYDAFIVYDTKDPAVTEWVLAELVAKLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTDKYAKTENFKIAFYLSHQRLMDEKVDVIILIFLEKPFQKSKFLQLRKRLCGSSVLEWPTNPQAHPYFWQCLKNALATDNHVAYSQVFKETV 8C701E9E437F2721 tlr3_human Toll-like receptor 3 TLR3 protein protein human Homo sapiens Human MRQTLPCIYFWGGLLPFGMLCASSTTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAANFTRYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFCTNLTELHLMSNSIQKIKNNPFVKQKNLITLDLSHNGLSSTKLGTQVQLENLQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIKEFSPGCFHAIGRLFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLKWTNLTMLDLSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLFNVRYLNLKRSFTKQSISLASLPKIDDFSFQWLKCLEHLNMEDNDIPGIKSNMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKISKIESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTRNSFALVPSLQRLMLRRVALKNVDSSPSPFQPLRNLTILDLSNNNIANINDDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKGLSHLHILNLESNGFDEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLITSVEKKVFGPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELSSHYLCNTPPHYHGFPVRLFDTSSCKDSAPFELFFMINTSILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRQTEQFEYAAYIIHAYKDKDWVWEHFSSMEKEDQSLKFCLEERDFEAGVFELEAIVNSIKRSRKIIFVITHHLLKDPLCKRFKVHHAVQQAIEQNLDSIILVFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERIGAFRHKLQVALGSKNSVH DIP protein O15455 original sequence version: 76 034E05ECA7A4D2F7 tlr8_human Toll-like receptor 8 TLR8 UNQ249/PRO286 protein protein human Homo sapiens Human MENMFLQSSMLTCIFLLISGSCELCAEENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVTAVILFFFTFFITTMVMLAALAHHLFYWDVWFIYNVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESRDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTVFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQTLRNVVLTENDSRYNNMYVDSIKQY 39A38B60629291C8 tlr9_human Toll-like receptor 9 TLR9 UNQ5798/PRO19605 protein protein human Homo sapiens Human MGFCRSALHPLSLLVQAIMLAMTLALGTLPAFLPCELQPHGLVNCNWLFLKSVPHFSMAAPRGNVTSLSLSSNRIHHLHDSDFAHLPSLRHLNLKWNCPPVGLSPMHFPCHMTIEPSTFLAVPTLEELNLSYNNIMTVPALPKSLISLSLSHTNILMLDSASLAGLHALRFLFMDGNCYYKNPCRQALEVAPGALLGLGNLTHLSLKYNNLTVVPRNLPSSLEYLLLSYNRIVKLAPEDLANLTALRVLDVGGNCRRCDHAPNPCMECPRHFPQLHPDTFSHLSRLEGLVLKDSSLSWLNASWFRGLGNLRVLDLSENFLYKCITKTKAFQGLTQLRKLNLSFNYQKRVSFAHLSLAPSFGSLVALKELDMHGIFFRSLDETTLRPLARLPMLQTLRLQMNFINQAQLGIFRAFPGLRYVDLSDNRISGASELTATMGEADGGEKVWLQPGDLAPAPVDTPSSEDFRPNCSTLNFTLDLSRNNLVTVQPEMFAQLSHLQCLRLSHNCISQAVNGSQFLPLTGLQVLDLSHNKLDLYHEHSFTELPRLEALDLSYNSQPFGMQGVGHNFSFVAHLRTLRHLSLAHNNIHSQVSQQLCSTSLRALDFSGNALGHMWAEGDLYLHFFQGLSGLIWLDLSQNRLHTLLPQTLRNLPKSLQVLRLRDNYLAFFKWWSLHFLPKLEVLDLAGNQLKALTNGSLPAGTRLRRLDVSCNSISFVAPGFFSKAKELRELNLSANALKTVDHSWFGPLASALQILDVSANPLHCACGAAFMDFLLEVQAAVPGLPSRVKCGSPGQLQGLSIFAQDLRLCLDEALSWDCFALSLLAVALGLGVPMLHHLCGWDLWYCFHLCLAWLPWRGRQSGRDEDALPYDAFVVFDKTQSAVADWVYNELRGQLEECRGRWALRLCLEERDWLPGKTLFENLWASVYGSRKTLFVLAHTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILSPDGRRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGMALTRDNHHFYNRNFCQGPTAE 71280AA9680EDCE2 tlr1-tlr1-1 2 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-1, Table 1 gypa-gypa-3 2 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2Ai, Table 1 Positive control tlr1-tlr1-2 2 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O60603:p.Gly593Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Wed May 02 19:32:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly593ala' Tue Jun 30 18:04:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly593Ala' O60603:p.Ala597Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V Wed May 02 15:36:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala597val' Tue Jun 30 15:55:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala597Val' O60603:p.Cys609Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C I Wed May 02 15:54:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys609ile' Tue Jun 30 19:16:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys609Ile' 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O60603:p.Gly593Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:49:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly593ala' Tue Jun 30 20:23:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly593Ala' O60603:p.Ala597Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V Wed May 02 15:42:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala597val' Tue Jun 30 17:34:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala597Val' O60603:p.Cys609Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C I Wed May 02 18:38:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys609ile' Tue Jun 30 17:33:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys609Ile' physical association physical association Figure 2A-2, 7A, table 1 tlr2-tlr2-1 2 15 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-3, table 1 tlr3-tlr3-1 2 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-4, table 1 tlr5-tlr5-1 2 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-5, table 1 tlr5-tlr5-2 2 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-6, table 1 tlr7-tlr7-1 2 14 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-7, table 1 tlr7-tlr7-2 2 16 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-8, table 1 tlr9-tlr9-1 2 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-9, table 1 tlr9-tlr9-2 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2A-10, table 1 tlr1-tlr1-3 5 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-1 tlr1-tlr1-4 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-2 tlr1-tlr2-2 5 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-3 tlr1-tlr6-1 5 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-4 tlr1-tlr1-5 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60603:p.Gly593Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 18:45:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly593ala' Tue Jun 30 16:49:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly593Ala' O60603:p.Ala597Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A V Wed May 02 15:30:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala597val' Tue Jun 30 15:37:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala597Val' O60603:p.Cys609Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C I Wed May 02 17:44:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys609ile' Tue Jun 30 16:43:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys609Ile' 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-1i, 7B tlr1-tlr2-3 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60603:p.Gly593Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Wed May 02 19:58:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly593ala' Tue Jun 30 19:22:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly593Ala' O60603:p.Ala597Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Wed May 02 20:09:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala597val' Tue Jun 30 16:01:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala597Val' O60603:p.Cys609Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C I Wed May 02 14:49:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys609ile' Tue Jun 30 16:31:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys609Ile' physical association physical association Figure 3-2i, 7B tlr1-tlr6-2 5 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed O60603:p.Gly593Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:34:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly593ala' Tue Jun 30 19:38:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly593Ala' O60603:p.Ala597Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Wed May 02 16:32:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala597val' Tue Jun 30 17:44:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala597Val' O60603:p.Cys609Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C I Wed May 02 19:01:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys609ile' Tue Jun 30 18:43:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys609Ile' tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-3i, 7B tlr1-tlr10-3 5 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed O60603:p.Gly593Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 19:30:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly593ala' Tue Jun 30 17:12:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly593Ala' O60603:p.Ala597Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A V Wed May 02 16:44:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala597val' Tue Jun 30 18:34:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala597Val' O60603:p.Cys609Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C I Wed May 02 17:13:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys609ile' Tue Jun 30 17:20:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys609Ile' tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-4i,7B tlr1-tlr2-4 5 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-1ii tlr1-tlr2-5 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-2ii tlr1-tlr6-3 5 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-3ii tlr10-tlr2-2 5 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mutated ToxR*_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain functional ToxR domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3-4ii tlr1-tlr1-6 1 10 unspecified role unspecified role neutral component neutral component purified purified n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Figure 5-1 Apparent Kd-646 (in terms of a dimensionless detergent:peptide ratio). A weak interaction. tlr2-tlr2-2 1 8 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Figure 5-2 Apparent Kd-4476 (in terms of a dimensionless detergent:peptide ratio). A moderately strong interaction. tlr6-tlr6-1 1 12 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Figure 5-3 Apparent Kd-884 (in terms of a dimensionless detergent:peptide ratio). A weak interaction. tlr6-tlr6-2 3 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role fluorescence donor fluorescence donor purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Figure 6(C) tlr6-tlr2-1 3 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role fluorescence donor fluorescence donor purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Figure 6(C)i lmp1-lmp1-1 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed Q1HVB3:p.Asp150Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:41:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp150ala' Tue Jun 30 19:13:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp150Ala' tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed Q1HVB3:p.Asp150Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:22:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp150ala' Tue Jun 30 20:15:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp150Ala' tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 7, Table 1 Positive control tlr2-tlr2-3 4 8 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Supp. Figure S3 Results showed that TLR2 exists in a monomer-dimer-tetramer equilibrium. I dont know how to enter stoichiometry for a situation like this, so have not. tlr6-tlr6-3 4 12 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Supp. Figure 4 TLR6 exists in a monomer-dimer equilibrium. tlr6-tlr2-2 4 8 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role neutral component neutral component purified purified tm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus coumarin label coumarin label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Supp fig 5 The average molecular weight obtained from the fit indicates that TLR6 shifts the monomer-dimer equilibrium of TLR2. This indicates that TLR2 and TLR6 are interacting.