IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. PLoS genetics 2012 imex curation Xie J., Peng M., Guillemette S., Quan S., Maniatis S., Wu Y., Venkatesh A., Shaffer SA., Brosh RM., Cantor SB. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added the acetylation assay reported in Fig.2A since it has not been performed using purified proteins. Sharon.Cantor@umassmed.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-fa-j Homo sapiens fa-j fa-j anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie J., Peng M., Guillemette S., Quan S., Maniatis S., Wu Y., Venkatesh A., Shaffer SA., Brosh RM., Cantor SB. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added the acetylation assay reported in Fig.2A since it has not been performed using purified proteins. Sharon.Cantor@umassmed.edu sharon.cantor@umasmed.edu Accepted 2018-JUL-13 AT 10:45 BST AT 10:45 BST by PPORRAS PLoS genetics 2012 Only protein-protein interactions imex curation imex curation All done FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. PLoS genetics 2012 imex curation Xie J., Peng M., Guillemette S., Quan S., Maniatis S., Wu Y., Venkatesh A., Shaffer SA., Brosh RM., Cantor SB. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added the acetylation assay reported in Fig.2A since it has not been performed using purified proteins. Sharon.Cantor@umassmed.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie J., Peng M., Guillemette S., Quan S., Maniatis S., Wu Y., Venkatesh A., Shaffer SA., Brosh RM., Cantor SB. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added the acetylation assay reported in Fig.2A since it has not been performed using purified proteins. Sharon.Cantor@umassmed.edu sharon.cantor@umasmed.edu Accepted 2018-JUL-13 AT 10:45 BST AT 10:45 BST by PPORRAS PLoS genetics 2012 Only protein-protein interactions imex curation imex curation All done FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. PLoS genetics 2012 imex curation Xie J., Peng M., Guillemette S., Quan S., Maniatis S., Wu Y., Venkatesh A., Shaffer SA., Brosh RM., Cantor SB. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added the acetylation assay reported in Fig.2A since it has not been performed using purified proteins. Sharon.Cantor@umassmed.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-fa-j Homo sapiens fa-j fa-j confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xie J., Peng M., Guillemette S., Quan S., Maniatis S., Wu Y., Venkatesh A., Shaffer SA., Brosh RM., Cantor SB. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added in vitro acetylated assay (Fig 2 B) since the construct for HATp300 was not provided and was not retrievable from the literature. I haven't added the acetylation assay reported in Fig.2A since it has not been performed using purified proteins. Sharon.Cantor@umassmed.edu sharon.cantor@umasmed.edu Accepted 2018-JUL-13 AT 10:45 BST AT 10:45 BST by PPORRAS PLoS genetics 2012 Only protein-protein interactions imex curation imex curation All done brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY DIP protein P38398 original sequence version: 183 DIP protein P38398 original sequence version: 111 DIP protein P38398 original sequence version: 149 DIP protein P38398-1 original sequence version: 149 DIP protein P38398 original sequence version: 142 DIP protein P38398 original sequence version: 212 DIP protein P38398 original sequence version: 163 DIP protein P38398 original sequence version: 185 DIP protein P38398 original sequence version: 148 DIP protein P38398 original sequence version: 210 89C6D83FF56312AF fancj_human Fanconi anemia group J protein BRIP1 BACH1 FANCJ ATP-dependent RNA helicase BRIP1 BRCA1-associated C-terminal helicase 1 BRCA1-interacting protein C-terminal helicase 1 protein protein human Homo sapiens Human MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKACPYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARDELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKLLEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLDFSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWGALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLEVTSLKYSTSPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKAEKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDCIDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK DIP protein Q9BX63 original sequence version: 81 8ED691F03A442BE1 mlh1_human DNA mismatch repair protein Mlh1 MutL protein homolog 1 MLH1 COCA2 protein protein human Homo sapiens Human MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC DIP protein P40692 original sequence version: 99 C9231FC406C2CA20 fancj-brca1-1 2 5 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 3A Zeocin and MMC treatment did not measurably alter FANCJ co-precipitation with BRCA1. Zeocin induces acetylation of FANCJ. UV treatment reduces FANCJ co-precipitation with BRCA1, it could reflect the UV-induced BRCA1 degradation. brip1-brca1-7 1 4 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait living cell living cell infection infection Q9BX63:p.Lys1249Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:08:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1249arg' Tue Jun 30 15:36:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1249Arg' Q9BX63:p.Lys1249Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K Q Wed May 02 18:27:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1249gln' Tue Jun 30 16:35:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1249Gln' association association Fig. 4B Similar to FANCJWT, FANCJK1249R and FANCJK1249Q precipitated with known FANCJ interacting partners, BRCA1 and MLH1 [6], [8] (Figure 4B). In addition, the mutants co-localized with BRCA1 in response to DNA damage and the FA-J cells expressing FANCJWT or mutants had similar asynchronous cell cycle profiles (Figure 4C, 4D). brip1-brca1-8 3 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 5 unspecified role unspecified role neutral component neutral component infection infection fixed cell fixed cell Q9BX63:p.Lys1249Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:30:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1249arg' Tue Jun 30 18:22:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1249Arg' Q9BX63:p.Lys1249Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K Q Wed May 02 14:55:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1249gln' Tue Jun 30 17:05:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1249Gln' colocalization colocalization Fig. 4D Similar to FANCJWT, FANCJK1249R and FANCJK1249Q precipitated with known FANCJ interacting partners, BRCA1 and MLH1 [6], [8] (Figure 4B). In addition, the mutants co-localized with BRCA1 in response to DNA damage and the FA-J cells expressing FANCJWT or mutants had similar asynchronous cell cycle profiles (Figure 4C, 4D).