IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function. Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function. European journal of immunology 2008 imex curation Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M., Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M. alemoret@unige.it 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M., Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M. alemoret@unige.it Accepted 2018-APR-07 AT 07:56 BST AT 07:56 BST by LIVIAP European journal of immunology 2008 Only protein-protein interactions imex curation imex curation Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function. Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function. European journal of immunology 2008 imex curation Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M., Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M. alemoret@unige.it 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M., Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M. alemoret@unige.it Accepted 2018-APR-07 AT 07:56 BST AT 07:56 BST by LIVIAP European journal of immunology 2008 Only protein-protein interactions imex curation imex curation Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function. Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function. European journal of immunology 2008 imex curation Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M., Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M. alemoret@unige.it 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M., Della Chiesa M., Romeo E., Falco M., Balsamo M., Augugliaro R., Moretta L., Bottino C., Moretta A., Vitale M. KIR2DL1- and KIR2DS1- Fc tagged constructs are stated to be "soluble" receptors; this has been assumed to correspond to the extracellular domain, although this construct would have a shorter sequence than the stated 245 aas for these KIRs. alemoret@unige.it Accepted 2018-APR-07 AT 07:56 BST AT 07:56 BST by LIVIAP European journal of immunology 2008 Only protein-protein interactions imex curation imex curation ki2s1_human Killer cell immunoglobulin-like receptor 2DS1 KIR2DS1 CD158H CD158 antigen-like family member H MHC class I NK cell receptor Eb6 ActI protein protein human Homo sapiens Human MSLTVVSMACVGFFLLQGAWPHEGVHRKPSLLAHPGRLVKSEETVILQCWSDVMFEHFLLHREGMFNDTLRLIGEHHDGVSKANFSISRMKQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVIIGLYEKPSLSAQPGPTVLAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAGTKVNGTFQANFPLGPATHGGTYRCFGSFRDSPYEWSKSSDPLLVSVTGNPSNSWPSPTEPSSETGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA FD323A0EB48B13C2 ki2s5_human Killer cell immunoglobulin-like receptor 2DS5 KIR2DS5 CD158G NKAT9 CD158 antigen-like family member G MHC class I NK cell receptor Natural killer-associated transcript 9 protein protein human Homo sapiens Human MSLMVISMACVAFFLLQGAWPHEGFRRKPSLLAHPGPLVKSEETVILQCWSDVMFEHFLLHREGTFNHTLRLIGEHIDGVSKGNFSIGRMTQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVITGLYEKPSLSAQPGPTVLAGESVTLSCSSRSSYDMYHLSREGEAHERRLPAGPKVNRTFQADFPLDPATHGGTYRCFGSFRDSPYEWSKSSDPLLVSVTGNSSNSWPSPTEPSSETGNPRHLHVLIGTSVVKLPFTILLFFLLHRWCSNKKNASVMDQGPAGNRTVNREDSDEQDHQEVSYA 83AFBB6A08D8DC9B hlac_human HLA class I histocompatibility antigen, C alpha chain HLA-Cw Human leukocyte antigen C HLAC HLA-C protein protein human Homo sapiens Human MRVMAPRALLLLLSGGLALTETWACSHSMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTIPIMGIVAGLAVLVVLAVLGAVVTAMMCRRKSSGGKGGSCSQAACSNSAQGSDESLITCKA DIP protein P30504 original sequence version: 126 59C23D95FD1D0BC8 tyobp_human TYRO protein tyrosine kinase-binding protein TYROBP DAP12 KARAP DNAX-activation protein 12 Killer-activating receptor-associated protein protein protein human Homo sapiens Human MGGLEPCSRLLLLPLLLAVSGLRPVQAQAQSDCSCSTVSPGVLAGIVMGDLVLTVLIALAVYFLGRLVPRGRGAAEAATRKQRITETESPYQELQGQRSDVYSDLNTQRPYYK 267CB1C1756F89F0 ki2l1_human Killer cell immunoglobulin-like receptor 2DL1 KIR2DL1 CD158 antigen-like family member A CD158A NKAT1 MHC class I NK cell receptor Natural killer-associated transcript 1 p58 natural killer cell receptor clones CL-42/47.11 p58.1 MHC class-I-specific NK receptor protein protein human Homo sapiens Human MSLLVVSMACVGFFLLQGAWPHEGVHRKPSLLAHPGPLVKSEETVILQCWSDVMFEHFLLHREGMFNDTLRLIGEHHDGVSKANFSISRMTQDLAGTYRCYGSVTHSPYQVSAPSDPLDIVIIGLYEKPSLSAQPGPTVLAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAGPKVNGTFQADFPLGPATHGGTYRCFGSFHDSPYEWSKSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHILIGTSVVIILFILLFFLLHRWCSNKKNAAVMDQESAGNRTANSEDSDEQDPQEVTYTQLNHCVFTQRKITRPSQRPKTPPTDIIVYTELPNAESRSKVVSCP mint 3DFBF2D6FDCE1D1E kir2ds5-tyrobp-1 3 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Figure 1D kir2ds5-tyrobp-2 1 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Figure 1E tyrobp-kir2ds5-1 2 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Figure 1Ei 1235445y7q3atsrh-1 3 8 unspecified role unspecified role neutral component neutral component partially purified partially purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range region fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line Human 721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line physical association physical association Figure 3 The KIR2DL1-Fc receptor strongly reacted with HLA-Cw4 and HLA-Cw6 cell transfectants. hla_c-kir2dl1-25 3 8 unspecified role unspecified role neutral component neutral component partially purified partially purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range region fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line Human 721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line physical association physical association Figure 3i The KIR2DL1-Fc receptor strongly reacted with HLA-Cw4 and HLA-Cw6 cell transfectants. the KIR2DL1-Fc receptor strongly reacted with HLA-Cw4 and HLA-Cw6 cell transfectants hla_c-kir2dl1-26 3 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line Human 721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line 4 unspecified role unspecified role neutral component neutral component partially purified partially purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range region fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Figure 3ii KIR2DS1-Fc proteins reacted strongly with cells transfected with the HLA-Cw6 allele. I have not added any (weak) interaction between KIR2DS1 and HLA-Cw4 - as there is no more interaction shown (less, in fact) than on the figure above (Cw3), which is stated to be negative.