IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Molecular endocrinology (Baltimore, Md.) 2008 imex curation Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca Accepted 2018-MAY-09 AT 11:51 BST AT 11:51 BST by ORCHARD Molecular endocrinology (Baltimore, Md.) 2008 imex curation Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Molecular endocrinology (Baltimore, Md.) 2008 imex curation Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca Accepted 2018-MAY-09 AT 11:51 BST AT 11:51 BST by ORCHARD Molecular endocrinology (Baltimore, Md.) 2008 imex curation Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Molecular endocrinology (Baltimore, Md.) 2008 imex curation Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca Accepted 2018-MAY-09 AT 11:51 BST AT 11:51 BST by ORCHARD Molecular endocrinology (Baltimore, Md.) 2008 imex curation Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Molecular endocrinology (Baltimore, Md.) 2008 imex curation Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro sumoylase assay sumoylase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca Accepted 2018-MAY-09 AT 11:51 BST AT 11:51 BST by ORCHARD Molecular endocrinology (Baltimore, Md.) 2008 imex curation Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Molecular endocrinology (Baltimore, Md.) 2008 imex curation Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca Accepted 2018-MAY-09 AT 11:51 BST AT 11:51 BST by ORCHARD Molecular endocrinology (Baltimore, Md.) 2008 imex curation Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif. Molecular endocrinology (Baltimore, Md.) 2008 imex curation Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Tremblay AM., Wilson BJ., Yang XJ., Giguère V. Currently, UniProt lists Human SUMO2 as a protein of 95 amino acids, and SUMO3 with 103 amino acids. Reference 49 (pmid:11451954) gives the sequences (figure 1) and primers used for SUMO2 and SUMO3 proteins; LAE BIO is the supplier for SUMO proteins. In Ref 49 the sequence for stated SUMO3 protein corresponds to Uniprot entry for SUMO2, and for the SUMO2 protein sequence corresponds to SUMO3. LAE BIO gives an obsolete Uniprot accession number (P55855) for "SUMO3" which corresponds to the current Uniprot entry for SUMO2. Thus when SUMO2 protein has been stated to have been used, a UniProt accession for SUMO3 has been entered and vice-versa. The interaction in mouse-liver (Fig 7D) has not been annotated due to lack of antibody specificity;"anti-SUMO2/3 antibody (no. AB3876, Chemicon)" was used fig 7d: although they state it is "SUMO2" in fig 7D, with the problems associated (see caution) with IDs of Sumo proteins and lack of antibody specificity, probably safer to omit. vincent.giguere@mcgill.ca Accepted 2018-MAY-09 AT 11:51 BST AT 11:51 BST by ORCHARD Molecular endocrinology (Baltimore, Md.) 2008 imex curation ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS DIP protein P63279 original sequence version: 33 DIP protein P63279 original sequence version: 57 DIP protein P63279 original sequence version: 80 DIP protein P63279 original sequence version: 137 DIP protein P63279 original sequence version: 99 DIP protein P63279 original sequence version: 140 DIP protein P63279 original sequence version: 120 DIP protein P63279 original sequence version: 117 DIP protein P63279 original sequence version: 93 E2C826E9C8D0683D pias4_human E3 SUMO-protein ligase PIAS4 Protein inhibitor of activated STAT protein gamma PIASy PIAS4 PIASG Protein inhibitor of activated STAT protein 4 RING-type E3 ubiquitin transferase PIAS4 protein protein human Homo sapiens Human MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC DIP protein Q8N2W9 original sequence version: 122 26AAA18246E1ACE3 err1_human Steroid hormone receptor ERR1 Estrogen-related receptor alpha Estrogen receptor-like 1 Nuclear receptor subfamily 3 group B member 1 ESRRA ERR1 ESRL1 NR3B1 protein protein human Homo sapiens Human MSSQVVGIEPLYIKAEPASPDSPKGSSETETEPPVALAPGPAPTRCLPGHKEEEDGEGAGPGEQGGGKLVLSSLPKRLCLVCGDVASGYHYGVASCEACKAFFKRTIQGSIEYSCPASNECEITKRRRKACQACRFTKCLRVGMLKEGVRLDRVRGGRQKYKRRPEVDPLPFPGPFPAGPLAVAGGPRKTAAPVNALVSHLLVVEPEKLYAMPDPAGPDGHLPAVATLCDLFDREIVVTISWAKSIPGFSSLSLSDQMSVLQSVWMEVLVLGVAQRSLPLQDELAFAEDLVLDEEGARAAGLGELGAALLQLVRRLQALRLEREEYVLLKALALANSDSVHIEDAEAVEQLREALHEALLEYEAGRAGPGGGAERRRAGRLLLTLPLLRQTAGKVLAHFYGVKLEGKVPMHKLFLEMLEAMMD DIP protein P11474 original sequence version: 102 BAE62DAF0BE6BA96 err3_human Estrogen-related receptor gamma ESRRG ERR3 ERRG2 KIAA0832 NR3B3 ERR gamma-2 Estrogen receptor-related protein 3 Nuclear receptor subfamily 3 group B member 3 protein protein human Homo sapiens Human MDSVELCLPESFSLHYEEELLCRMSNKDRHIDSSCSSFIKTEPSSPASLTDSVNHHSPGGSSDASGSYSSTMNGHQNGLDSPPLYPSAPILGGSGPVRKLYDDCSSTIVEDPQTKCEYMLNSMPKRLCLVCGDIASGYHYGVASCEACKAFFKRTIQGNIEYSCPATNECEITKRRRKSCQACRFMKCLKVGMLKEGVRLDRVRGGRQKYKRRIDAENSPYLNPQLVQPAKKPYNKIVSHLLVAEPEKIYAMPDPTVPDSDIKALTTLCDLADRELVVIIGWAKHIPGFSTLSLADQMSLLQSAWMEILILGVVYRSLSFEDELVYADDYIMDEDQSKLAGLLDLNNAILQLVKKYKSMKLEKEEFVTLKAIALANSDSMHIEDVEAVQKLQDVLHEALQDYEAGQHMEDPRRAGKMLMTLPLLRQTSTKAVQHFYNIKLEGKVPMHKLFLEMLEAKV 63D36CFD37573152 sae2_human SUMO-activating enzyme subunit 2 Ubiquitin-like modifier-activating enzyme 2 HRIHFB2115 Ubiquitin-like 1-activating enzyme E1B Anthracycline-associated resistance ARX UBA2 SAE2 UBLE1B protein protein human Homo sapiens Human MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD DIP protein Q9UBT2 original sequence version: 85 C12D15293BBF90EB erre_probe consensus ERR response element (ERRE) probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human TCGACGCTTTCAAGGTCATATCCG sae1_human SUMO-activating enzyme subunit 1 Ubiquitin-like 1-activating enzyme E1A SAE1 AOS1 UBLE1A SUA1 protein protein human Homo sapiens Human MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK DIP protein Q9UBE0 original sequence version: 86 E2B10A69FF2ED746 sumo3_human Small ubiquitin-related modifier 3 SMT3 homolog 1 SUMO-2 SMT3H1 SUMO3 SMT3A Ubiquitin-like protein SMT3A protein protein human Homo sapiens Human MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVPESSLAGHSF DIP protein P55854 original sequence version: 56 DIP protein P55854 original sequence version: 127 DIP protein P55854 original sequence version: 106 DIP protein P55854 original sequence version: 124 DIP protein P55854 original sequence version: 99 E337E34CB5B3B187 sumo1_human Small ubiquitin-related modifier 1 SUMO1 Sentrin Ubiquitin-like protein SMT3C Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 SMT3 homolog 3 SMT3C SMT3H3 UBL1 OK/SW-cl.43 protein protein human Homo sapiens Human MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV DIP protein P63165 original sequence version: 36 DIP protein P63165 original sequence version: 40 DIP protein P63165 original sequence version: 124 DIP protein P63165 original sequence version: 125 DIP protein P63165 original sequence version: 83 DIP protein P63165 original sequence version: 85 DIP protein P63165 original sequence version: 81 DIP protein P63165 original sequence version: 84 DIP protein P63165 original sequence version: 103 DIP protein P63165 original sequence version: 100 DIP protein P63165 original sequence version: 95 DIP protein P63165 original sequence version: 98 DIP protein P63165 original sequence version: 101 DIP protein P63165-1 original sequence version: 131 DIP protein P63165 original sequence version: 102 DIP protein P63165 original sequence version: 122 DIP protein P63165 original sequence version: 62 DIP protein P63165 original sequence version: 119 DIP protein P63165 original sequence version: 82 89BE97D2D054FB33 prgc1_human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Ligand effect modulator 6 PPARGC1A LEM6 PGC1 PGC1A PPARGC1 protein protein human Homo sapiens Human MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR DIP protein Q9UBK2 original sequence version: 127 DIP protein Q9UBK2 original sequence version: 104 97DDD3643051A5F1 sumo2_human Small ubiquitin-related modifier 2 SMT3 homolog 2 Sentrin-2 HSMT3 SUMO-3 SUMO2 SMT3H2 SMT3B Ubiquitin-like protein SMT3B protein protein human Homo sapiens Human MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY DIP protein P61956 original sequence version: 39 DIP protein P61956 original sequence version: 79 DIP protein P61956 original sequence version: 81 DIP protein P61956 original sequence version: 77 DIP protein P61956 original sequence version: 98 DIP protein P61956 original sequence version: 116 DIP protein P61956 original sequence version: 141 F8F0426849BEF08B esrra-sumo3-1 2 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1C esrra-sumo2-1 2 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1D esrra-sae1-3 4 15 enzyme enzyme neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 ancillary ancillary neutral component neutral component purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 ancillary ancillary neutral component neutral component purified purified 13 ancillary ancillary neutral component neutral component purified purified 9 enzyme target enzyme target substrate substrate neutral component neutral component partially purified partially purified region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11474:p.Lys14Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:53:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys14arg' Tue Jun 30 16:26:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys14Arg' P11474:p.Lys403Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:15:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys403arg' Tue Jun 30 20:05:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys403Arg' in vitro In vitro sumoylation sumoylation reaction sumoylation Figure 2A LHS It is stated that ERRa K403R was sumoylated to a level similar to the wild-type receptor, however there is a missing band for the ERRa K403R mutant. esrra-sae1-1 4 17 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 7 ancillary ancillary neutral component neutral component purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 ancillary ancillary neutral component neutral component purified purified 13 ancillary ancillary neutral component neutral component purified purified 9 enzyme target enzyme target substrate substrate neutral component neutral component partially purified partially purified region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11474:p.Lys14Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 19:01:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys14arg' Tue Jun 30 17:22:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys14Arg' P11474:p.Lys403Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 17:22:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys403arg' Tue Jun 30 18:20:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys403Arg' in vitro In vitro sumoylation sumoylation reaction sumoylation Figure 2A-RHS It is stated that ERRa K403R was sumoylated to a level similar to the wild-type receptor, however there is a missing band for the ERRa K403R mutant. esrra-sae1-2 4 15 enzyme enzyme neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 ancillary ancillary neutral component neutral component purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 ancillary ancillary neutral component neutral component purified purified 13 ancillary ancillary neutral component neutral component purified purified 10 enzyme target enzyme target substrate substrate neutral component neutral component partially purified partially purified region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P62508:p.Lys360Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:02:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys360arg' Tue Jun 30 17:52:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys360Arg' P62508:p.Lys439Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:34:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys439arg' Tue Jun 30 17:40:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys439Arg' P62508:p.Lys40Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:10:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys40arg' Tue Jun 30 19:27:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Arg' in vitro In vitro sumoylation sumoylation reaction sumoylation Figure 2B LHS It is stated that ERRa K403R was sumoylated to a level similar to the wild-type receptor, however there is a missing band for the ERRa K403R mutant. esrrg-sae1-1 4 17 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 7 ancillary ancillary neutral component neutral component purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 ancillary ancillary neutral component neutral component purified purified 13 ancillary ancillary neutral component neutral component purified purified 10 enzyme target enzyme target substrate substrate neutral component neutral component partially purified partially purified region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P62508:p.Lys360Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 17:59:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys360arg' Tue Jun 30 17:57:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys360Arg' P62508:p.Lys439Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:02:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys439arg' Tue Jun 30 17:19:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys439Arg' P62508:p.Lys40Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:09:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys40arg' Tue Jun 30 19:57:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Arg' in vitro In vitro sumoylation sumoylation reaction sumoylation Figure 2B RHS It is stated that ERRa K403R was sumoylated to a level similar to the wild-type receptor, however there is a missing band for the ERRa K403R mutant. ppargc1a-esrra-14 6 16 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey partially purified partially purified region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11474:p.Lys14Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 19:01:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys14arg' Tue Jun 30 18:46:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys14Arg' in vitro In vitro physical association physical association Figure 3I ppargc1a-esrra-13 6 16 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey partially purified partially purified region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P62508:p.Lys40Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:20:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys40arg' Tue Jun 30 15:40:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Arg' in vitro In vitro physical association physical association Figure 3I-2 esrra-sumo3-2 5 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P11474:p.Lys14Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 19:49:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys14Arg' 14 immunocytochemistry immunocytochemistry tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figure 4A I dont think colocalisation is meant to be "nuclear" - text not very clear, but it does not look like there is much nuclear staining to me. 45646-1 1 9 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11474:p.Lys403Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 17:55:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys403arg' Tue Jun 30 20:19:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys403Arg' P11474:p.Lys14Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:22:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys14arg' Tue Jun 30 18:29:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys14Arg' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association figure 4B i have not entered the GST-PGC-1a addition, as ERRalpha is still present for these assays, and we do not know what the probe is binding to (ERRa or PGC-1a or both) in the absence of antibody being used. -same for rest of the assays - no antibody used. No change in the DNA binding pattern was observed by addition of purified GST-PGC-1a/1–250 fusion protein. pias4-esrra-1 3 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sumoylated lysine sumoylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6B A band that is stated to show probable sumoylation of ERRa on Lys-14 is shown. there is no specific antibody for sumo-erra that I can find, so no definite proof it is a sumo band. pias4-esrra-2 3 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11474:p.Lys14Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed Jun 13 18:51:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys14arg' Tue Jun 30 18:48:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys14Arg' physical association physical association Figure 6Bi No sumoylation is shown.