UniProt UniProt http://www.uniprot.org Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-n2a Mouse neuroblastoma cells n2a Murine neuroblastoma cells fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret antibody detection identification by antibody antibody detection Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease Fluorescence donor/acceptors were on secondary Abs. willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease Cells were treated with membrane-permeable linker dithiobis-succinimidyl-propionate before coimmunopreciptiation with anti-SORL1 willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease Cells were treated with membrane-permeable cross-linkers that penetrate the undisturbed cellular compartments and link APP- and sorLA-interacting proteins in the context of an intact cell. willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Andersen OM., Reiche J., Schmidt V., Gotthardt M., Spoelgen R., Behlke J., von Arnim CA., Breiderhoff T., Jansen P., Wu X., Bales KR., Cappai R., Masters CL., Gliemann J., Mufson EJ., Hyman BT., Paul SM., Nykjaer A., Willnow TE. Alzheimers - Interactions investigated in the context of Alzheimers disease willnow@mdc-berlin.de Accepted 2019-JAN-21 AT 17:12 GMT AT 17:12 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation sorl_human Sortilin-related receptor Sorting protein-related receptor containing LDLR class A repeats SorLA-1 Low-density lipoprotein receptor relative with 11 ligand-binding repeats SORL1 C11orf32 protein protein human Homo sapiens Human MATRSSRRESRLPFLFTLVALLPPGALCEVWTQRLHGGSAPLPQDRGFLVVQGDPRELRLWARGDARGASRADEKPLRRKRSAALQPEPIKVYGQVSLNDSHNQMVVHWAGEKSNVIVALARDSLALARPKSSDVYVSYDYGKSFKKISDKLNFGLGNRSEAVIAQFYHSPADNKRYIFADAYAQYLWITFDFCNTLQGFSIPFRAADLLLHSKASNLLLGFDRSHPNKQLWKSDDFGQTWIMIQEHVKSFSWGIDPYDKPNTIYIERHEPSGYSTVFRSTDFFQSRENQEVILEEVRDFQLRDKYMFATKVVHLLGSEQQSSVQLWVSFGRKPMRAAQFVTRHPINEYYIADASEDQVFVCVSHSNNRTNLYISEAEGLKFSLSLENVLYYSPGGAGSDTLVRYFANEPFADFHRVEGLQGVYIATLINGSMNEENMRSVITFDKGGTWEFLQAPAFTGYGEKINCELSQGCSLHLAQRLSQLLNLQLRRMPILSKESAPGLIIATGSVGKNLASKTNVYISSSAGARWREALPGPHYYTWGDHGGIITAIAQGMETNELKYSTNEGETWKTFIFSEKPVFVYGLLTEPGEKSTVFTIFGSNKENVHSWLILQVNATDALGVPCTENDYKLWSPSDERGNECLLGHKTVFKRRTPHATCFNGEDFDRPVVVSNCSCTREDYECDFGFKMSEDLSLEVCVPDPEFSGKSYSPPVPCPVGSTYRRTRGYRKISGDTCSGGDVEARLEGELVPCPLAEENEFILYAVRKSIYRYDLASGATEQLPLTGLRAAVALDFDYEHNCLYWSDLALDVIQRLCLNGSTGQEVIINSGLETVEALAFEPLSQLLYWVDAGFKKIEVANPDGDFRLTIVNSSVLDRPRALVLVPQEGVMFWTDWGDLKPGIYRSNMDGSAAYHLVSEDVKWPNGISVDDQWIYWTDAYLECIERITFSGQQRSVILDNLPHPYAIAVFKNEIYWDDWSQLSIFRASKYSGSQMEILANQLTGLMDMKIFYKGKNTGSNACVPRPCSLLCLPKANNSRSCRCPEDVSSSVLPSGDLMCDCPQGYQLKNNTCVKQENTCLRNQYRCSNGNCINSIWWCDFDNDCGDMSDERNCPTTICDLDTQFRCQESGTCIPLSYKCDLEDDCGDNSDESHCEMHQCRSDEYNCSSGMCIRSSWVCDGDNDCRDWSDEANCTAIYHTCEASNFQCRNGHCIPQRWACDGDTDCQDGSDEDPVNCEKKCNGFRCPNGTCIPSSKHCDGLRDCSDGSDEQHCEPLCTHFMDFVCKNRQQCLFHSMVCDGIIQCRDGSDEDAAFAGCSQDPEFHKVCDEFGFQCQNGVCISLIWKCDGMDDCGDYSDEANCENPTEAPNCSRYFQFRCENGHCIPNRWKCDRENDCGDWSDEKDCGDSHILPFSTPGPSTCLPNYYRCSSGTCVMDTWVCDGYRDCADGSDEEACPLLANVTAASTPTQLGRCDRFEFECHQPKTCIPNWKRCDGHQDCQDGRDEANCPTHSTLTCMSREFQCEDGEACIVLSERCDGFLDCSDESDEKACSDELTVYKVQNLQWTADFSGDVTLTWMRPKKMPSASCVYNVYYRVVGESIWKTLETHSNKTNTVLKVLKPDTTYQVKVQVQCLSKAHNTNDFVTLRTPEGLPDAPRNLQLSLPREAEGVIVGHWAPPIHTHGLIREYIVEYSRSGSKMWASQRAASNFTEIKNLLVNTLYTVRVAAVTSRGIGNWSDSKSITTIKGKVIPPPDIHIDSYGENYLSFTLTMESDIKVNGYVVNLFWAFDTHKQERRTLNFRGSILSHKVGNLTAHTSYEISAWAKTDLGDSPLAFEHVMTRGVRPPAPSLKAKAINQTAVECTWTGPRNVVYGIFYATSFLDLYRNPKSLTTSLHNKTVIVSKDEQYLFLVRVVVPYQGPSSDYVVVKMIPDSRLPPRHLHVVHTGKTSVVIKWESPYDSPDQDLLYAVAVKDLIRKTDRSYKVKSRNSTVEYTLNKLEPGGKYHIIVQLGNMSKDSSIKITTVSLSAPDALKIITENDHVLLFWKSLALKEKHFNESRGYEIHMFDSAMNITAYLGNTTDNFFKISNLKMGHNYTFTVQARCLFGNQICGEPAILLYDELGSGADASATQAARSTDVAAVVVPILFLILLSLGVGFAILYTKHRRLQSSFTAFANSHYSSRLGSAIFSSGDDLGEDDEDAPMITGFSDDVPMVIA DIP protein Q92673 original sequence version: 154 4C215BB33E65C0B2 p05067-1 Amyloid-beta precursor protein APP A4 AD1 ABPP APPI Alzheimer disease amyloid protein Amyloid precursor protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II PreA4 770 Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN A major isoform. A12EE761403740F5 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 p05067-4 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 695 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067-4 original sequence version: 183 DIP protein P05067-4 original sequence version: 198 DIP protein P05067-4 original sequence version: 224 A major isoform. 4F6EA0139F969D56 p05067-8 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 751 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAIPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN A major isoform. C987C557C5A3714E sorl1-app-1 2 11 unspecified role unspecified role prey prey purified purified extracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role bait bait purified purified ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Fig. 1B When the sorLA ectodomain immobilized on the biosensor chip was incubated with increasing concentrations of APP695, a rapid and reversible increase in response units indicated specific interaction of the two proteins on the chip surface (Fig. 1B). sorl1-app-2 2 12 unspecified role unspecified role prey prey purified purified extracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role bait bait purified purified ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Fig. 8 sorl1-app-3 2 9 unspecified role unspecified role prey prey purified purified extracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role bait bait purified purified ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Fig. 8 sorl1-app-4 3 11 unspecified role unspecified role neutral component neutral component purified purified extracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role neutral component neutral component purified purified ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Fig. 1C app-sorl1-4 6 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Fig. 2E When anti-APP immunoprecipitates were probed with an anti-sorLA antibody, coprecipitation of sorLA with APP antiserum was detected in CHO-A/S cells that express both proteins but not in CHO-S cells that lack APP (Fig. 2E). sorl1-app-6 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Fig. 2F Similarly, when anti-sorLA immunoprecipitates were probed with anti-APP antibodies, coprecipitation of APP with sorLA antiserum was detected in CHO-A/S cells but not in CHO-A cells that lack sorLA (Fig. 2F). sorl1-app-5 7 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed colocalization colocalization Fig. 2A, Supp Fig. 9A Colocalisation observed when cells were permeabilised with Triton X-100 prior to Ab detection In cells expressing APP and sorLA (CHO-A/S), both proteins colocalized to vesicular structures and to the perinuclear region (Fig. 2A, arrowhead). app-sorl1-6 7 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig. 2B, 2C Colocalisation observed irrespective of whether cells were permeabilised with Triton X-100 prior to Ab detection app-sorl1-7 1 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed extracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. proximity proximity close-contact colocalization neighbourhood interaction Fig. 3B, 3C sorl1-app-7 4 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 4A Despite low amounts of endogenous sorLA expressed in these cells (“Input” in Fig. 4A), coprecipitation of endogenous APP with anti-sorLA but not with nonimmune IgG was possible (“IP” in Fig. 4A), confirming the findings in CHO cells. sorl1-app-8 5 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Fig. 4B Despite low amounts of endogenous sorLA expressed in these cells (“Input” in Fig. 4A), coprecipitation of endogenous APP with anti-sorLA but not with nonimmune IgG was possible (“IP” in Fig. 4A), confirming the findings in CHO cells.