IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Recognition of nectin-2 by the natural killer cell receptor TIGIT. Recognition of nectin-2 by the natural killer cell receptor TIGIT. The Journal of biological chemistry 2017 imex curation Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu Accepted 2017-SEP-15 AT 09:20 BST AT 09:20 BST by ORCHARD The Journal of biological chemistry 2017 imex curation Recognition of nectin-2 by the natural killer cell receptor TIGIT. Recognition of nectin-2 by the natural killer cell receptor TIGIT. The Journal of biological chemistry 2017 imex curation Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu Accepted 2017-SEP-15 AT 09:20 BST AT 09:20 BST by ORCHARD The Journal of biological chemistry 2017 imex curation Recognition of nectin-2 by the natural killer cell receptor TIGIT. Recognition of nectin-2 by the natural killer cell receptor TIGIT. The Journal of biological chemistry 2017 imex curation Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu Accepted 2017-SEP-15 AT 09:20 BST AT 09:20 BST by ORCHARD The Journal of biological chemistry 2017 imex curation Recognition of nectin-2 by the natural killer cell receptor TIGIT. Recognition of nectin-2 by the natural killer cell receptor TIGIT. The Journal of biological chemistry 2017 imex curation Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293s Human 293S cell line Human 293s 293S cell line molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Deuss FA., Gully BS., Rossjohn J., Berry R. Richard.berry@monash.edu,Jamie.rossjohn@monash.edu Accepted 2017-SEP-15 AT 09:20 BST AT 09:20 BST by ORCHARD The Journal of biological chemistry 2017 imex curation pvr_human Poliovirus receptor PVR PVS Nectin-like protein 5 protein protein human Homo sapiens Human MARAMAAAWPLLLVALLVLSWPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGISRNAIIFLVLGILVFLILLGIGIYFYWSKCSREVLWHCHLCPSSTEHASASANGHVSYSAVSRENSSSQDPQTEGTR DIP protein P15151 original sequence version: 140 D15C012CE853169B nect2_human Nectin-2 NECTIN2 HVEB Herpes virus entry mediator B Nectin cell adhesion molecule 2 PRR2 PVRL2 Poliovirus receptor-related protein 2 protein protein human Homo sapiens Human MARAAALLPSRSPPTPLLWPLLLLLLLETGAQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVSGTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDATLSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETPNTAGAGATGGIIGGIIAAIIATAVAATGILICRQQRKEQTLQGAEEDEDLEGPPSYKPPTPKAKLEAQEMPSQLFTLGASEHSPLKTPYFDAGASCTEQEMPRYHELPTLEERSGPLHPGATSLGSPIPVPPGPPAVEDVSLDLEDEEGEEEEEYLDKINPIYDALSYSSPSDSYQGKGFVMSRAMYV DIP protein Q92692 original sequence version: 131 DIP protein Q92692 original sequence version: 134 3AE4F83E92F6F624 tigit_human T-cell immunoreceptor with Ig and ITIM domains TIGIT VSIG9 VSTM3 V-set and immunoglobulin domain-containing protein 9 V-set and transmembrane domain-containing protein 3 protein protein human Homo sapiens Human MRWCLLLIWAQGLRQAPLASGMMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIPLLGAMAATLVVICTAVIVVVALTRKKKALRIHSVEGDLRRKSAGQEEWSPSAPSPPGSCVQAEAAPAGLCGEQRGEDCAELHDYFNVLSYRSLGNCSFFTETG B890AF73F618C851 nectin2-tigit-1 1 6 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s Human 293S cell line Human 293s 293S cell line 7 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q495A1:p.Cys69Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:37:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ser' Tue Jun 30 18:04:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys69Ser' ecoli Escherichia coli (strain K12) 13 10 direct interaction direct interaction 1B nectin2-tigit-3 3 6 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q92692:p.Met89Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Wed May 02 16:58:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met89ala' Tue Jun 30 17:44:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met89Ala' Q92692:p.Gly90Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 17:41:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly90ala' Tue Jun 30 16:48:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly90Ala' Q92692:p.Glu101Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:52:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu101ala' Tue Jun 30 19:21:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu101Ala' Q92692:p.Glu141Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:08:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu141ala' Tue Jun 30 17:30:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu141Ala' Q92692:p.Phe145Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:27:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe145ala' Tue Jun 30 19:47:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe145Ala' Q92692:p.Leu67Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 19:59:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu67gln' Tue Jun 30 19:42:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu67Gln' Q92692:p.Leu67Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:45:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu67ala' Tue Jun 30 18:35:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu67Ala' Q92692:p.Ala83Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Wed May 02 17:02:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala83val' Tue Jun 30 17:58:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala83Val' Q92692:p.Ala83Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A R Wed May 02 19:45:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala83arg' Tue Jun 30 19:30:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala83Arg' Q92692:p.His86Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Wed May 02 16:11:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his86ala' Tue Jun 30 18:45:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His86Ala' human-293s Human 293S cell line Human 293s 293S cell line 7 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q495A1:p.Cys69Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:14:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ser' Tue Jun 30 19:59:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys69Ser' c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 1C, 5 nectin2-7 4 6 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1B-2 nectin2-tigit-2 3 6 unspecified role unspecified role prey prey first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s Human 293S cell line Human 293s 293S cell line 7 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q495A1:p.Cys69Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 20:28:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ser' Tue Jun 30 20:07:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys69Ser' ecoli Escherichia coli (strain K12) 39 43 direct interaction direct interaction 1C-2 nectin2-tigit-4 3 5 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q495A1:p.Cys69Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:06:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ser' Tue Jun 30 15:43:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys69Ser' c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C-3 nectin2-tigit-5 2 6 unspecified role unspecified role neutral component neutral component first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s Human 293S cell line Human 293s 293S cell line 7 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q495A1:p.Cys69Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:46:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ser' Tue Jun 30 16:43:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys69Ser' ecoli Escherichia coli (strain K12) 55 58 direct interaction direct interaction Table 1 3.1A RWork- 0.218 RFree = 0.236