IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Analytical chemistry 2017 imex curation Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn Accepted 2017-OCT-18 AT 09:34 BST AT 09:34 BST by ORCHARD Analytical chemistry 2017 imex curation done; done; I have added 293T cells for "expressed-ins" as I missed this before in the Methods section - I have not seen any other IPs -so must refer to here. So cannot be "Direct".; I have altered Interaction detection back to "Electrophoresis" (-unless you can think of something better?) as it was "sls" - static light scattering, which would seem unlikely. also type to Direct. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Analytical chemistry 2017 imex curation Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis western blot western blot Immuno blot Immuno blot Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn Accepted 2017-OCT-18 AT 09:34 BST AT 09:34 BST by ORCHARD Analytical chemistry 2017 imex curation done; done; I have added 293T cells for "expressed-ins" as I missed this before in the Methods section - I have not seen any other IPs -so must refer to here. So cannot be "Direct".; I have altered Interaction detection back to "Electrophoresis" (-unless you can think of something better?) as it was "sls" - static light scattering, which would seem unlikely. also type to Direct. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Analytical chemistry 2017 imex curation Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro biosensor biosensor predetermined predetermined participant predetermined Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? Dual-polarization interferometry ( DPI ) is an analytical technique that probes molecular layers adsorbed to the surface of a waveguide using the evanescent wave of a laser beam. xryang@ciac.ac.cn,jiansun@ciac.ac.cn Accepted 2017-OCT-18 AT 09:34 BST AT 09:34 BST by ORCHARD Analytical chemistry 2017 imex curation done; done; I have added 293T cells for "expressed-ins" as I missed this before in the Methods section - I have not seen any other IPs -so must refer to here. So cannot be "Direct".; I have altered Interaction detection back to "Electrophoresis" (-unless you can think of something better?) as it was "sls" - static light scattering, which would seem unlikely. also type to Direct. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Real-Time Analysis of Binding Events between Different Aβ1-42 Species and Human Lilrb2 by Dual Polarization Interferometry. Analytical chemistry 2017 imex curation Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Hu T., Wang S., Chen C., Sun J., Yang X. I cannot get any sensible results out of the Sangon Biotechnology Co. Ltd. (Shanghai, China) website for LILRB2 constructs. The Manual says "The fields “Min Stoich” and “Max Stoich ” should have the same values entered –a range is only entered for Complex curation." - but I HAVE entered ranges here, as would appear to be clearcut? xryang@ciac.ac.cn,jiansun@ciac.ac.cn Accepted 2017-OCT-18 AT 09:34 BST AT 09:34 BST by ORCHARD Analytical chemistry 2017 imex curation done; done; I have added 293T cells for "expressed-ins" as I missed this before in the Methods section - I have not seen any other IPs -so must refer to here. So cannot be "Direct".; I have altered Interaction detection back to "Electrophoresis" (-unless you can think of something better?) as it was "sls" - static light scattering, which would seem unlikely. also type to Direct. lirb2_human Leukocyte immunoglobulin-like receptor subfamily B member 2 LILRB2 ILT4 LIR2 MIR10 CD85 antigen-like family member D Immunoglobulin-like transcript 4 Monocyte/macrophage immunoglobulin-like receptor 10 protein protein human Homo sapiens Human MTPIVTVLICLGLSLGPRTHVQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVVLLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKDTQPEDGVEMDTRAAASEAPQDVTYAQLHSLTLRRKATEPPPSQEREPPAEPSIYATLAIH DIP protein Q8N423 original sequence version: 127 DIP protein Q8N423 original sequence version: 104 33E9737AFCAFCE2D p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 app-114 4 6 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 1 Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer. app-115 2 6 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. Figure 1 Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer. app-116 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Supp. figure 2 (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) All forms of Beta-amyloid protein 42 all bound to GFP-ED1D2L. lilrb2-app-1 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Supp. figure 2i (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) All forms of Beta-amyloid protein 42 all bound to GFP-ED1D2L. lilrb2-app-2 3 5 unspecified role unspecified role bait bait purified purified ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 3,4 Table 1, supp fig 5/6 (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) lilrb2-app-3 3 5 unspecified role unspecified role bait bait purified purified ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 3,4 Table 1, Supp fig 5/6 (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) lilrb2-app-4 3 5 unspecified role unspecified role bait bait purified purified ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 3,4 Table 1, Supp Fig 5/6. (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.)