IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein. Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein. Journal of hematology & oncology 2017 imex curation Chen Y., Zhang S., Wang Q., Zhang X. as far as I can make out, the exact cell lines used are not specified for the first 2 expts. The only stated version of rCHI3L1 is Human in origin (supplement) so I assume the GST tagged version will be so as well. zxb0812@zju.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Chen Y., Zhang S., Wang Q., Zhang X. as far as I can make out, the exact cell lines used are not specified for the first 2 expts. The only stated version of rCHI3L1 is Human in origin (supplement) so I assume the GST tagged version will be so as well. GST-CHI3L1 purified protein was incubated with solubilized membrane proteins of gastric and breast cancer cells. zxb0812@zju.edu.cn Accepted 2017-SEP-13 AT 11:38 BST AT 11:38 BST by PPORRAS Journal of hematology & oncology 2017 imex curation There is only ONE IP - and they do not specify which cell line was used for this - the cell lines given at the top are for the Input/expression only. Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein. Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein. Journal of hematology & oncology 2017 imex curation Chen Y., Zhang S., Wang Q., Zhang X. as far as I can make out, the exact cell lines used are not specified for the first 2 expts. The only stated version of rCHI3L1 is Human in origin (supplement) so I assume the GST tagged version will be so as well. zxb0812@zju.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Chen Y., Zhang S., Wang Q., Zhang X. as far as I can make out, the exact cell lines used are not specified for the first 2 expts. The only stated version of rCHI3L1 is Human in origin (supplement) so I assume the GST tagged version will be so as well. Recombinant CHI3L1 purified protein was incubated with MDA-MB-231 cells. zxb0812@zju.edu.cn Accepted 2017-SEP-13 AT 11:38 BST AT 11:38 BST by PPORRAS Journal of hematology & oncology 2017 imex curation There is only ONE IP - and they do not specify which cell line was used for this - the cell lines given at the top are for the Input/expression only. Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein. Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein. Journal of hematology & oncology 2017 imex curation Chen Y., Zhang S., Wang Q., Zhang X. as far as I can make out, the exact cell lines used are not specified for the first 2 expts. The only stated version of rCHI3L1 is Human in origin (supplement) so I assume the GST tagged version will be so as well. zxb0812@zju.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen Y., Zhang S., Wang Q., Zhang X. as far as I can make out, the exact cell lines used are not specified for the first 2 expts. The only stated version of rCHI3L1 is Human in origin (supplement) so I assume the GST tagged version will be so as well. Recombinant CHI3L1 purified protein was incubated with gastric and breast cancer cells. Cell membrane proteins were then extracted and incubated with antibodies. zxb0812@zju.edu.cn Accepted 2017-SEP-13 AT 11:38 BST AT 11:38 BST by PPORRAS Journal of hematology & oncology 2017 imex curation There is only ONE IP - and they do not specify which cell line was used for this - the cell lines given at the top are for the Input/expression only. i13r2_human Interleukin-13 receptor subunit alpha-2 IL13RA2 IL13R Interleukin-13-binding protein protein protein human Homo sapiens Human MAFVCLAIGCLYTFLISTTFGCTSSSDTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYELKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKVQDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDFYICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDDTTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLLRFWLPFGFILILVIFVTGLLLRKPNTYPKMIPEFFCDT DIP protein Q14627 original sequence version: 99 3C6ACB1B5562C887 p36222-pro_0000011965 Chitinase-3-like protein 1 39 kDa synovial protein Cartilage glycoprotein 39 YKL-40 CHI3L1 protein protein human Homo sapiens Human YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT 22 383 BE87C12A36F45673 ch3l1_human Chitinase-3-like protein 1 CHI3L1 39 kDa synovial protein Cartilage glycoprotein 39 YKL-40 protein protein human Homo sapiens Human MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT 6C9EF133BDC7C2D1 567uru-1 1 4 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 4A chi3l1-il13ra2-1 3 4 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association 4B chi3l1-il13ra2-2 2 4 unspecified role unspecified role neutral component neutral component human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma 5 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection colocalization colocalization 4C