IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS pathogens 2017 imex curation Kinosada H., Yasunaga JI., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K., Matsuoka M. The reference strain of HTLV-1 has been used as no strain is specified either here or Ref 58. mmatsuok@virus.kyoto-u.ac.jp,jyasunag@virus.kyoto-u.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-jurkat Homo sapiens jurkat Human T cell leukemia confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Kinosada H., Yasunaga JI., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K., Matsuoka M. HBZ:Ref 58 refers back to several other publications and has several primers, but the wild geese are probably flying... The reference strain of HTLV-1 has been used as no strain is specified either here or Ref 58. mmatsuok@virus.kyoto-u.ac.jp,jyasunag@virus.kyoto-u.ac.jp Accepted 2017-SEP-07 AT 09:58 BST AT 09:58 BST by ORCHARD PLoS pathogens 2017 imex curation HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS pathogens 2017 imex curation Kinosada H., Yasunaga JI., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K., Matsuoka M. The reference strain of HTLV-1 has been used as no strain is specified either here or Ref 58. mmatsuok@virus.kyoto-u.ac.jp,jyasunag@virus.kyoto-u.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kinosada H., Yasunaga JI., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K., Matsuoka M. HBZ:Ref 58 refers back to several other publications and has several primers, but the wild geese are probably flying... The reference strain of HTLV-1 has been used as no strain is specified either here or Ref 58. mmatsuok@virus.kyoto-u.ac.jp,jyasunag@virus.kyoto-u.ac.jp Accepted 2017-SEP-07 AT 09:58 BST AT 09:58 BST by ORCHARD PLoS pathogens 2017 imex curation HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS pathogens 2017 imex curation Kinosada H., Yasunaga JI., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K., Matsuoka M. The reference strain of HTLV-1 has been used as no strain is specified either here or Ref 58. mmatsuok@virus.kyoto-u.ac.jp,jyasunag@virus.kyoto-u.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Kinosada H., Yasunaga JI., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K., Matsuoka M. HBZ:Ref 58 refers back to several other publications and has several primers, but the wild geese are probably flying... The reference strain of HTLV-1 has been used as no strain is specified either here or Ref 58. mmatsuok@virus.kyoto-u.ac.jp,jyasunag@virus.kyoto-u.ac.jp Accepted 2017-SEP-07 AT 09:58 BST AT 09:58 BST by ORCHARD PLoS pathogens 2017 imex curation pdcd1_human Programmed cell death protein 1 PDCD1 PD1 protein protein human Homo sapiens Human MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL DIP protein Q15116 original sequence version: 143 A5210FD40C304FB7 grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 hbz_htl1c HTLV-1 basic zipper factor HBZ protein protein htl1c Human T-cell leukemia virus 1 (isolate Caribbea HS-35 subtype A) HTLV-1 MVNFVSVGLFRCLPVPCPEDLLVEELVDGLLSLEEELKDKEEEETVLDGLLSLEEESRGRLRRGPPGGKAPPRGETHRDRQRRAEEKRKRKKEREKEEEKQIAEYLKRKEEEKARRRKRAEEKAADFARRKQEEQERRERKWRQGAEKAKQHSARKEKMQELGVDGYTRQLEGEVESLEAERRRLLQEKEDLMGEVNYWQGRLEAMWLQ 55323CF9189A7D54 ptn11_human Tyrosine-protein phosphatase non-receptor type 11 PTPN11 PTP2C SHPTP2 Protein-tyrosine phosphatase 2C Protein-tyrosine phosphatase 1D SH-PTP3 SH-PTP2 protein protein human Homo sapiens Human MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR 9CDBEFFA5E6CCB45 thms1_human Protein THEMIS THEMIS C6orf190 C6orf207 Thymocyte-expressed molecule involved in selection protein protein human Homo sapiens Human MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK 30B5325242212E94 pdcd1-ptpn11-1 1 4 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 5 Pervanadate (CHEBI:35607) Human T-cell leukemia virus type 1 (HTLV-1) HBZ protein SHP-2 co-localized with PD-1 in Jurkat cells after pervanadate stimulation themis-hbz-1 2 8 unspecified role unspecified role bait bait PA_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association figure 7A themis-hbz-2 2 5 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait PA_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 7B themis-grb2-16 2 5 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait PA_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 inhibitor inhibitor prey prey c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association figure 7B-2 ptpn11-pdcd1-1 2 7 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse_cd28_ecd+tm_domains tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B It is stated that "HBZ hinders recruitment of SHP-2 to the ITSM motif of PD-1.". From Ref. 40 - "colocalization of PD-1 with CD3 and/or CD28 may be necessary for inhibition of T cell activation." I see no evidence that SHP2 is binding to PD1 rather than mouse CD28- but the authors seem to think it is, so have left this interaction in, (and associated Colocalisation Experiment)- with mouse cd28 as a TAG rather than as a fusion protein as it is not meant to participate in the reaction. Or mCD28/PD1 fusion is "not a biologically relevant mol" so this interaction should not be added? Human T-cell leukemia virus 1 HBZ protein Pervanadate (CHEBI:35607) themis-grb2-17 3 8 unspecified role unspecified role neutral component neutral component PA_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization figure 8B When both proteins were expressed, HBZ was co-localized with THEMIS in the cytoplasm