DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269120X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269121X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269115X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269117X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269114X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269119X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269116X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature 2016 imex curation Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269118X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. a6nd01-1 Sperm-egg fusion protein Juno Folate receptor 4 Folate receptor delta IZUMO1 receptor protein JUNO IZUMO1R FOLR4 JUNO protein protein human Homo sapiens Human MACWWPLLLELWTVMPTWAGDELLNICMNAKHHKRVPSPEDKLYEECIPWKDNACCTLTTSWEAHLDVSPLYNFSLFHCGLLMPGCRKHFIQAICFYECSPNLGPWIQPVGSLGWEVAPSGQGERVVNVPLCQEDCEEWWEDCRMSYTCKSNWRGGWDWSQGKNRCPKGAQCLPFSHYFPTPADLCEKTWSNSFKASPERRNSGRCLQKWFEPAQGNPNVAVARLFASSAPSWELSYTIMVCSLFLPFLS DIP protein A6ND01-1 original sequence version: 65 No experimental confirmation available. Gene prediction based on EST data. q8iyv9-1 Izumo sperm-egg fusion protein 1 Oocyte binding/fusion factor Sperm-specific protein izumo IZUMO1 protein protein human Homo sapiens Human MGPHFTLLCAALAGCLLPAEGCVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLLGLLICGSLALITGLTFAIFRRRKVIDFIKSSLFGLGSGAAEQTQVPKEKATDSRQQ DIP protein Q8IYV9-1 original sequence version: 114 izumo1r-izumo1-1 7 9 unspecified role unspecified role bait bait Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role prey prey Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8IYV9-1:p.Trp148Ala W148A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W148A'. Wed May 02 15:43:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp148ala' Tue Jun 30 16:43:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp148Ala' Q8IYV9-1:p.Arg160Ala R160A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R160A'. Wed May 02 16:33:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg160ala' Tue Jun 30 17:30:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg160Ala' Q8IYV9-1:p.Arg160Glu R160E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R160E'. Wed May 02 16:43:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg160glu' Tue Jun 30 18:14:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg160Glu' Q8IYV9-1:p.His157Ala H157A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H157A'. Wed May 02 20:14:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his157ala' Tue Jun 30 18:16:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His157Ala' Q8IYV9-1:p.Glu71Ala E71A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E71A'. Wed May 02 17:06:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu71ala' Tue Jun 30 16:09:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu71Ala' Q8IYV9-1:p.Glu71Lys E71K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E71K'. Wed May 02 15:29:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu71lys' Tue Jun 30 17:21:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu71Lys' drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-6 5 9 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-7 2 9 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-8 8 9 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-9 3 9 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1r-izumo1-2 6 9 unspecified role unspecified role bait bait Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain A6ND01-1:p.Lys163Glu K163E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K163E'. Wed May 02 15:56:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys163glu' Tue Jun 30 19:02:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys163Glu' A6ND01-1:p.Lys163Ala K163A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K163A'. Wed May 02 14:50:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys163ala' Tue Jun 30 20:21:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys163Ala' A6ND01-1:p.Leu81Ala L81A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L81A'. Wed May 02 16:44:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu81ala' Tue Jun 30 19:35:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Ala' A6ND01-1:p.Trp62Ala W62A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W62A'. Wed May 02 19:57:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp62ala' Tue Jun 30 16:04:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp62Ala' A6ND01-1:p.Glu45Ala E45A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E45A'. Wed May 02 17:38:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu45ala' Tue Jun 30 19:52:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu45Ala' A6ND01-1:p.Glu45Lys E45K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E45K'. Wed May 02 16:40:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu45lys' Tue Jun 30 20:00:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu45Lys' drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role prey prey Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-10 1 9 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1r-izumo1-3 4 9 unspecified role unspecified role bait bait Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role prey prey Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction