DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269131X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269129X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269134X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269133X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269135X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269130X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269132X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269137X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269136X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science (New York, N.Y.) 2016 imex curation Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yi F., Danko T., Botelho SC., Patzke C., Pak C., Wernig M., Südhof TC. DIP original experiment: DIP-269138X Science (New York, N.Y.) 2016 IMEx Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. hcn1_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 HCN1 BCNG1 Brain cyclic nucleotide-gated channel 1 protein protein human Homo sapiens Human MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIAPINYPQMTTLNSTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLMQQQPQQQVQQSQPPQTQPQQPSPQPQTPGSSTPKNEVHKSTQALHNTNLTREVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL DIP protein O60741 original sequence version: 133 A62F5E79C01257A2 hcn2_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 HCN2 BCNG2 Brain cyclic nucleotide-gated channel 2 protein protein human Homo sapiens Human MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL DIP protein Q9UL51 original sequence version: 137 4B263E0C06C2A47D q4acu6-1 SH3 and multiple ankyrin repeat domains protein 3 Shank3 Kiaa1650 Prosap2 Proline-rich synapse-associated protein 2 SPANK-2 A Alpha protein protein mouse Mus musculus Mouse MDGPGASAVVVRVGIPDLQQTKCLRLDPTAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDEERLLQDYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLDPNFHDPDSGECPLSLAAQLDNATDLLKVLRNGGAHLDFRTRDGLTAVHCATRQRNAGALTTLLDLGASPDYKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGTTDENGWQEIHQACRFGHVQHLEHLLFYGANMGAQNASGNTALHICALYNQESCARVLLFRGANKDVRNYNSQTAFQVAIIAGNFELAEVIKTHKDSDVVPFRETPSYAKRRRLAGPSGLASPRPLQRSASDINLKGDQPAASPGPTLRSLPHQLLLQRLQEEKDRDRDGELENDISGPSAGRGGHNKISPSGPGGSGPAPGPGPASPAPPAPPPRGPKRKLYSAVPGRKFIAVKAHSPQGEGEIPLHRGEAVKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQYDTRHETREDRTKRLFRHYTVGSYDSLTSHSDYVIDDKVAILQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWRAGLRTGDFLIEVNGVNVVKVGHKQVVGLIRQGGNRLVMKVVSVTRKPEEDGARRRAPPPPKRAPSTTLTLRSKSMTAELEELASIRRRKGEKLDEILAVAAEPTLRPDIADADSRAATVKQRPTSRRITPAEISSLFERQGLPGPEKLPGSLRKGIPRTKSVGEDEKLASLLEGRFPRSTSMQDTVREGRGIPPPPQTAPPPPPAPYYFDSGPPPTFSPPPPPGRAYDTVRSSFKPGLEARLGAGAAGLYDPSTPLGPLPYPERQKRARSMIILQDSAPEVGDVPRPAPAATPPERPKRRPRPSGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPVGGSFAREPSPTHRGPRPGSLDYSSGEGLGLTFGGPSPGPVKERRLEERRRSTVFLSVGAIEGSPPSADLPSLQPSRSIDERLLGTGATTGRDLLLPSPVSALKPLVGGPSLGPSGSTFIHPLTGKPLDPSSPLALALAARERALASQTPSRSPTPVHSPDADRPGPLFVDVQTRDSERGPLASPAFSPRSPAWIPVPARREAEKPPREERKSPEDKKSMILSVLDTSLQRPAGLIVVHATSNGQEPSRLGAEEERPGTPELAPAPMQAAAVAEPMPSPRAQPPGSIPADPGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGILLTTPPPGPGPLPTTVPSPASGKPSSELPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFADGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHAASTGLASAAGPARPRYLFQRRSKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAARLFSSLGELSTISAQRSPGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLGAGVGGAGRPFGLTPPTILKSSSLSIPHEPKEVRFVVRSVSARSRSPSPSPLPSPSPGSGPSAGPRRPFQQKPLQLWSKFDVGDWLESIHLGEHRDRFEDHEIEGAHLPALTKEDFVELGVTRVGHRMNIERALRQLDGS DIP protein Q4ACU6-1 original sequence version: 107 hcn3_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 HCN3 KIAA1535 protein protein human Homo sapiens Human MEAEQRPAAGASEGATPGLEAVPPVAPPPATAASGPIPKSGPEPKRRHLGTLLQPTVNKFSLRVFGSHKAVEIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDLVLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPPDCWVSINHMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRGLVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLLRIGKKNSILQRKRSEPSPGSSGGIMEQHLVQHDRDMARGVRGRAPSTGAQLSGKPVLWEPLVHAPLQAAAVTSNVAIALTHQRGPLPLSPDSPATLLARSAWRSAGSPASPLVPVRAGPWASTSRLPAPPARTLHASLSRAGRSQVSLLGPPPGGGGRRLGPRGRPLSASQPSLPQRATGDGSPGRKGSGSERLPPSGLLAKPPRTAQPPRPPVPEPATPRGLQLSANM DIP protein Q9P1Z3 original sequence version: 134 DIP protein Q9P1Z3 original sequence version: 136 37B9BC13E5E2C097 shank3-hcn2-1 1 12 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association shank3-hcn1-1 2 11 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association hcn1-shank3-1 5 11 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-345 Region 1-345 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association shank3-hcn3-1 4 14 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association hcn3-shank3-1 8 14 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-345 Region 1-345 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association hcn2-shank3-1 7 12 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association shank3-hcn1-2 10 11 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-345 Region 1-345 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association hcn1-shank3-2 6 11 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association hcn2-shank3-2 9 12 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-345 Region 1-345 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association hcn3-shank3-2 3 14 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association