DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu USP7 is a SUMO deubiquitinase essential for DNA replication. USP7 is a SUMO deubiquitinase essential for DNA replication. Nature structural & molecular biology 2016 imex curation Lecona E., Rodriguez-Acebes S., Specks J., Lopez-Contreras AJ., Ruppen I., Murga M., Muñoz J., Mendez J., Fernandez-Capetillo O. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Lecona E., Rodriguez-Acebes S., Specks J., Lopez-Contreras AJ., Ruppen I., Murga M., Muñoz J., Mendez J., Fernandez-Capetillo O. DIP original experiment: DIP-268841X Nature structural & molecular biology 2016 IMEx USP7 is a SUMO deubiquitinase essential for DNA replication. USP7 is a SUMO deubiquitinase essential for DNA replication. USP7 is a SUMO deubiquitinase essential for DNA replication. Nature structural & molecular biology 2016 imex curation Lecona E., Rodriguez-Acebes S., Specks J., Lopez-Contreras AJ., Ruppen I., Murga M., Muñoz J., Mendez J., Fernandez-Capetillo O. Only protein-protein interactions in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Lecona E., Rodriguez-Acebes S., Specks J., Lopez-Contreras AJ., Ruppen I., Murga M., Muñoz J., Mendez J., Fernandez-Capetillo O. DIP original experiment: DIP-268840X Nature structural & molecular biology 2016 IMEx USP7 is a SUMO deubiquitinase essential for DNA replication. USP7 is a SUMO deubiquitinase essential for DNA replication. USP7 is a SUMO deubiquitinase essential for DNA replication. Nature structural & molecular biology 2016 imex curation Lecona E., Rodriguez-Acebes S., Specks J., Lopez-Contreras AJ., Ruppen I., Murga M., Muñoz J., Mendez J., Fernandez-Capetillo O. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lecona E., Rodriguez-Acebes S., Specks J., Lopez-Contreras AJ., Ruppen I., Murga M., Muñoz J., Mendez J., Fernandez-Capetillo O. DIP original experiment: DIP-268839X Nature structural & molecular biology 2016 IMEx USP7 is a SUMO deubiquitinase essential for DNA replication. sumo2_human Small ubiquitin-related modifier 2 SMT3 homolog 2 Sentrin-2 HSMT3 SUMO-3 SUMO2 SMT3H2 SMT3B Ubiquitin-like protein SMT3B protein protein human Homo sapiens Human MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY DIP protein P61956 original sequence version: 39 DIP protein P61956 original sequence version: 79 DIP protein P61956 original sequence version: 81 DIP protein P61956 original sequence version: 77 DIP protein P61956 original sequence version: 98 DIP protein P61956 original sequence version: 116 DIP protein P61956 original sequence version: 141 F8F0426849BEF08B mcm4_human DNA replication licensing factor MCM4 MCM4 CDC21 CDC21 homolog P1-CDC21 protein protein human Homo sapiens Human MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKTVRLL DIP protein P33991 original sequence version: 121 DIP protein P33991 original sequence version: 165 DIP protein P33991 original sequence version: 168 96D9CA2A7D88D015 ubp7_human Ubiquitin carboxyl-terminal hydrolase 7 USP7 HAUSP Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 Deubiquitinating enzyme 7 Herpesvirus-associated ubiquitin-specific protease protein protein human Homo sapiens Human MNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN DIP protein Q93009 original sequence version: 71 DIP protein Q93009 original sequence version: 67 DIP protein Q93009-1 original sequence version: 180 DIP protein Q93009 original sequence version: 173 DIP protein Q93009 original sequence version: 91 DIP protein Q93009 original sequence version: 163 DIP protein Q93009 original sequence version: 176 DIP protein Q93009-1 original sequence version: 128 DIP protein Q93009 original sequence version: 128 F1A5A5C421396E45 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG scml2_human Sex comb on midleg-like protein 2 SCML2 protein protein human Homo sapiens Human MGQTVNEDSMDVKKENQEKTPQSSTSSVQRDDFHWEEYLKETGSISAPSECFRQSQIPPVNDFKVGMKLEARDPRNATSVCIATVIGITGARLRLRLDGSDNRNDFWRLVDSPDIQPVGTCEKEGDLLQPPLGYQMNTSSWPMFLLKTLNGSEMASATLFKKEPPKPPLNNFKVGMKLEAIDKKNPYLICPATIGDVKGDEVHITFDGWSGAFDYWCKYDSRDIFPAGWCRLTGDVLQPPGTSVPIVKNIAKTESSPSEASQHSMQSPQKTTLILPTQQVRRSSRIKPPGPTAVPKRSSSVKNITPRKKGPNSGKKEKPLPVICSTSAASLKSLTRDRGMLYKDVASGPCKIVMSTVCVYVNKHGNFGPHLDPKRIQQLPDHFGPGPVNVVLRRIVQACVDCALETKTVFGYLKPDNRGGEVITASFDGETHSIQLPPVNSASFALRFLENFCHSLQCDNLLSSQPFSSSRGHTHSSAEHDKNQSAKEDVTERQSTKRSPQQTVPYVVPLSPKLPKTKEYASEGEPLFAGGSAIPKEENLSEDSKSSSLNSGNYLNPACRNPMYIHTSVSQDFSRSVPGTTSSPLVGDISPKSSPHEVKFQMQRKSEAPSYIAVPDPSVLKQGFSKDPSTWSVDEVIQFMKHTDPQISGPLADLFRQHEIDGKALFLLKSDVMMKYMGLKLGPALKLCYYIEKLKEGKYS DIP protein Q9UQR0 original sequence version: 116 DIP protein Q9UQR0 original sequence version: 137 05E086D4928DEE73 sumo2-ub-1 1 4 unspecified role unspecified role unspecified role unspecified role K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction usp7-mcm4-1 3 8 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait human Homo sapiens Human association association ub-usp7-2 2 7 enzyme target enzyme target substrate substrate unspecified role unspecified role K48-Ub K48-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain K63-Ub K63-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli deubiquitination deubiquitination reaction deubiquitination