DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PLoS biology 2016 imex curation Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. DIP original experiment: DIP-268530X PLoS biology 2016 IMEx PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PLoS biology 2016 imex curation Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. DIP original experiment: DIP-268529X PLoS biology 2016 IMEx PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PLoS biology 2016 imex curation Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. DIP original experiment: DIP-268531X PLoS biology 2016 IMEx PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PLoS biology 2016 imex curation Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang K., Liu Y., Fan J., Zhang J., Li XA., Evers BM., Zhu H., Jia J. DIP original experiment: DIP-268528X PLoS biology 2016 IMEx PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. gprk2_drome G protein-coupled receptor kinase 2 Gprk2 Gprk-2 CG17998 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCINLKDKLDISYGYVIDQQPIGRELFRLFCENKRPVYFRYITFLDEVVKYEIEYISNRIFIGHDIGRRFLDVEAQLELRNGSGGDALDAETQEELLLNSSNANPTETAETEHCNNTTANNCNNINNSNNSQHSSDINHKKLDTRNHNGDDATGNGSSHQDDGDESVKCQEGHDDAEKGGGGEGGGGGKCVPVGGYPDELVLDVLNDDLIAQVRNKLNSGGKDIFAQCVNAVKAFLAGEPFREFESSMYFHRYLQWKWLEAQPITYKTFRMYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSPFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGGEPGFELERARFYAAEVACGLQHLHKQGIVYRDCKPENILLDDHGHVRISDLGLAVEIPEGEMVRGRVGTVGYMAPEVIDNEKYAFSPDWFSFGCLLYEMIEGQAPFRMRKEKVKREEVDRRVKEDPEKYSSKFNDEAKSMCQQLLAKSIKQRLGCRNGRMGGQDVMAHPFFHSTQLNWRRLEAGMLEPPFVPDPHAVYAKDVLDIEQFSTVKGVNIDESDTNFYTKFNTGSVSISWQNEMMETECFRELNVFGPEECPTPDLQINAAPEPDKAGCFPFRRKKKQPARTQPIPIPEHLLTTSHSVSSTTVES DIP protein P32866 original sequence version: 139 103F853BC74CFD80 cos_drome Kinesin-like protein costa cos cos2 costal-2 Kinesin-like protein costal2 CG1708 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEIPIQVAVRIFPHRELKDLLRSFGPTEPKKDAQAVDEGADSKDSEAQVPAAEKDNPSISETDPNGNAEQDSAADSKTIPDANGNDSGQKDYPDSAYCVQAIPISASALGLPSALPGGDPMDSIAAGLIQVGPHTVPVTHALPSSSSQEQVYHQTVFPLITLFLEGFDASVVTYGQRGQGKSYTLYGNVQDPTLTDSTEGVVQLCVRDIFSHISLHPERTYAINVGFVEICGGDVCDLLGMGNIHCTNVDAVFHWLQVGLSARQSLPAHTLFTLTLEQQWVSKEGLLQHRLSTASFSDLCGTERCGDQPPGRPLDAGLCMLEQVISTLTDPGLMYGVNGNIPYGQTTLTTLLKDSFGGRAQTLVILCVSPLEEHLPETLGNLQFAFKVQCVRNFVIMNTYSDDNTMIVQPAEPVPESNSSAGPLSQAGPGDNFGLQFAASQWSKLVTNAEGLFSKLIDSKLITEVEKEQIEEWLFLKQECEECLSSTEAMRQQKQLVPILEAEEPEDVNSEAANSESPNSDNENDTDNESHRPDLDDKIESLMEEFRDKTDALILEKHAEYLSKHPKAVMQSQDREIEAQPPEENGDDRKVSIGSRRRSVQPGASLSTAELAMLNRVASQQPPPPIDPESVVDPLESSSGEGIRQAALAAAAATAPIEQLQKKLRKLVAEIEGKQRQLREIEETIQVKQNIIAELVKNSDTRSHAKQRFHKKRAKLEAECDKAKKQLGKALVQGRDQSEIERWTTIIGHLERRLEDLSSMKHIAGESGQKVKKLQQSVGESRKQADDLQKKLRKECKLRCQMEAELAKLRESRETGKELVKAQGSPEQQGRQLKAVQARITHLNHILREKSDNLEEQPGPEQQETLRHEIRNLRGTRDLLLEERCHLDRKLKRDKVLTQKEERKLLECDEAIEAIDAAIEFKNEMITGHRSIDTSDRIQREKGEQMLMARLNRLSTEEMRTLLYKYFTKVIDLRDSSRKLELQLVQLERERDAWEWKERVLSNAVRQARLEGERNAVLLQRQHEMKLTLMLRHMAEETSASSASYGERALAPACVAPPVQASSDFDYDHFYKGGGNPSKALIKAPKPMPTGSALDKYKDKEQRSGRNIFAKFHVLTRYASAAAAGSSGSTAEESTALIESTTTATATTTSTTTTGAVGKVKDKALVSFRPEQLKRLMPAPTATKVTRQKNKIIIQDASRRN DIP protein O16844 original sequence version: 88 DIP protein O16844 original sequence version: 100 DIP protein O16844 original sequence version: 121 6DC898F8B4C8F20F smo_drome Protein smoothened smo CG11561 dSMO SMOH Smooth protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQYLNFPRMPNIMMFLEVAILCLWVVADASASSAKFGSTTPASAQQSDVELEPINGTLNYRLYAKKGRDDKPWFDGLDSRHIQCVRRARCYPTSNATNTCFGSKLPYELSSLDLTDFHTEKELNDKLNDYYALKHVPKCWAAIQPFLCAVFKPKCEKINGEDMVYLPSYEMCRITMEPCRILYNTTFFPKFLRCNETLFPTKCTNGARGMKFNGTGQCLSPLVPTDTSASYYPGIEGCGVRCKDPLYTDDEHRQIHKLIGWAGSICLLSNLFVVSTFFIDWKNANKYPAVIVFYINLCFLIACVGWLLQFTSGSREDIVCRKDGTLRHSEPTAGENLSCIVIFVLVYYFLTAGMVWFVFLTYAWHWRAMGHVQDRIDKKGSYFHLVAWSLPLVLTITTMAFSEVDGNSIVGICFVGYINHSMRAGLLLGPLCGVILIGGYFITRGMVMLFGLKHFANDIKSTSASNKIHLIIMRMGVCALLTLVFILVAIACHVTEFRHADEWAQSFRQFIICKISSVFEEKSSCRIENRPSVGVLQLHLLCLFSSGIVMSTWCWTPSSIETWKRYIRKKCGKEVVEEVKMPKHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFDVNDLNSSETNDISSTWAAYLPQCVKRRMALTGAATGNSSSHGPRKNSLDSEISVSVRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRRDYIAAATGKSSRRRESSTSVESQVIALKKTTYPNASHKVGVFAHHSSKKQHNYTSSMKRRTANAGLDPSILNEFLQKNGDFIFPFLQNQDMSSSSEEDNSRASQKIQDLNVVVKQQEISEDDHDGIKIEELPNSKQVALENFLKNIKKSNESNSNRHSRNSARSQSKKSQKRHLKNPAADLDFRKDCVKYRSNDSLSCSSEELDVALDVGSLLNSSFSGISMGKPHSRNSKTSCDVGIQANPFELVPSYGEDELQQAMRLLNAASRQRTEAANEDFGGTELQGLLGHSHRHQREPTFMSESDKLKMLLLPSK DIP protein P91682 original sequence version: 121 DIP protein P91682 original sequence version: 141 DIP protein P91682 original sequence version: 105 7797FC71A539A87A fused_drome Serine/threonine-protein kinase fused fu CG6551 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNRYAVSSLVGQGSFGCVYKATRKDDSKVVAIKVISKRGRATKELKNLRRECDIQARLKHPHVIEMIESFESKTDLFVVTEFALMDLHRYLSYNGAMGEEPARRVTGHLVSALYYLHSNRILHRDLKPQNVLLDKNMHAKLCDFGLARNMTLGTHVLTSIKGTPLYMAPELLAEQPYDHHADMWSLGCIAYESMAGQPPFCASSILHLVKMIKHEDVKWPSTLTSECRSFLQGLLEKDPGLRISWTQLLCHPFVEGRIFIAETQAEAAKESPFTNPEAKVKSSKQSDPEVGDLDEALAALDFGESRQENLTTSRDSINAIAPSDVEHLETDVEDNMQRVVVPFADLSYRDLSGVRAMPMVHQPVINSHTCFVSGNSNMILNHMNDNFDFQASLRGGGVAAKPIVAPTVRQSRSKDLEKRKLSQNLDNFSVRLGHSVDHEAQRKATEIATQEKHNQENKPPAEAISYANSQPPQQQPQQLKHSMHSTNEEKLSSDNTPPCLLPGWDSCDESQSPPIENDEWLAFLNRSVQELLDGELDSLKQHNLVSIIVAPLRNSKAIPRVLKSVAQLLSLPFVLVDPVLIVDLELIRNVYVDVKLVPNLMYACKLLLSHKQLSDSAASAPLTTGSLSRTLRSIPELTVEELETACSLYELVCHLVHLQQQFLTQFCDAVAILAASDLFLNFLTHDFRQSDSDAASVRLAGCMLALMGCVLRELPENAELVERIVFNPRLNFVSLLQSRHHLLRQRSCQLLRLLARFSLRGVQRIWNGELRFALQQLSEHHSYPALRGEAAQTLDEISHFTFFVT DIP protein P23647 original sequence version: 121 DIP protein P23647 original sequence version: 147 A7804E2749B79D0C q9vc05_drome Oxysterol-binding protein Osbp CG6708 Dmel\CG6708 OSBP OSBP-Dm osbp Dmel_CG6708 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTDAAGNALAEKGLPEMKGWLLKWTNYIKGYQRRWFVLSKGVLSYYRNQSEINHTCRGTISLHGALIHTVDSCTFVISNGGTQTFHIKAGTEVERQSWVTALELAKAKAIRAIECEEEEETETAHVVPSQEISSVVRDLTDRLESMRTCYDLITKHGAALQRALNDLETNEEESLGSRTKIVNERATLFRITSNAMINAGNDYLHTAEAQGHKWSKMLHHEREQRQRLEEIIEQMAKQQSQMEQAAVLVRQNKPVSSTSGAGTAGSLVTSDEEMEFFDAEEHGYSGSGRSPESSDCERGTFILKMHKRRSSSEDQVEGHLEGSSSESDEQKRTVQTVQQVCLVSAPRVASGGQGDDDDDVDKALPAKESTDSIYGRNWNPDLIKKRRDRVPDKPNHPISLWGIMKNCIGKDLSKIPMPINFNEPLSMLQRLVEDYEYTEILDYAATCQDECEQLAYIAAFTVSAYATTTNRTGKPFNPLLGETYECDRMDDYGWRCLAEQVSHHPPVAALHCESKNWTCWQEFSMTSKFRGKYVQINPLGGVYVQFPNSGRRYSWRKVTTTVNNIIVGKLWVDQHGEMEIRGSQAAEGHKCILNFIPYSYFSRDVQRSVKGVVMNKDNEVKWVVRGTWDMKIEIAPVLKTSGSVSSPTYTTGEFKLAWRRRPAPPDSDKFYNFTTLACQLNEEEEGVAPTDSRLRPDQRLMEQGDWDESNKEKLRLEEKQRTERRRRENEAEEAAAEGRPYPAYEPMWFKREKEEGSEEYVHVFKNTYWEAKAAQNFEGCPDIY DIP protein Q9VC05 original sequence version: 126 E11429C115C0C80D smo-osbp-1 4 9 unspecified role unspecified role prey prey Region 17-123 Region 17-123 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association smo-gprk2-1 1 5 unspecified role unspecified role prey prey Region 667-714 Region 667-714 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association smo-cos-5 2 8 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 6 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P91682:p.[Arg736Ala;Arg737Ala;Arg738Ala] R736A/R737A/R738A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R736A/R737A/R738A'. Wed May 02 14:53:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg736ala,arg737ala,arg738ala' Tue Jun 30 19:52:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg736Ala;Arg737Ala;Arg738Ala]' drome Drosophila melanogaster (Fruit fly) Fruit fly association association smo-6 3 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly proximity proximity close-contact colocalization neighbourhood interaction