IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1. Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1. Cell 2016 imex curation Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF. EBOLA protein:-PDBe use the nearest reviewed entry - P87666 which has ~16 aas different from the one quoted in Supplement- 97.6% ID. I dont know where PDBe found their GPcl aa numbers from (they use 32-188 and 509-632-which does bear some resemblance to the Dube-2009 publication numbering. The EBOV GP accession number is given in the Supplement:- KJ660347.1 which corresponds (100% identity) to the sequence in Uniprot entry X5HMX4. The exact sequence of "Primed GPcl" is not given. Dube et al 2009 state that cathepsin cleavage results in the GP protein containing residues 33 to 190 of GP 1 and all residues of GP 2 (502-676). gaof@im.ac.cn 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF. EBOLA protein:-PDBe use the nearest reviewed entry - P87666 which has ~16 aas different from the one quoted in Supplement- 97.6% ID. I dont know where PDBe found their GPcl aa numbers from (they use 32-188 and 509-632-which does bear some resemblance to the Dube-2009 publication numbering. To mimic endosomal protease cleavage and produce primed EBOV GP (GPcl) which was capable of NPC1 binding, EBOV GP-ecto-Delta-mucin protein was incubated with thermolysin. The EBOV GP accession number is given in the Supplement:- KJ660347.1 which corresponds (100% identity) to the sequence in Uniprot entry X5HMX4. The exact sequence of "Primed GPcl" is not given. Dube et al 2009 state that cathepsin cleavage results in the GP protein containing residues 33 to 190 of GP 1 and all residues of GP 2 (502-676). gaof@im.ac.cn Accepted 2017-MAR-08 AT 08:53 GMT AT 08:53 GMT by ORCHARD Cell 2016 imex curation I have added the extra Expt and also altered all the viral constructs so that the TWO "Sufficient to Bind" regions have been amalgamated into ONE - throughout the publication. Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1. Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1. Cell 2016 imex curation Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF. EBOLA protein:-PDBe use the nearest reviewed entry - P87666 which has ~16 aas different from the one quoted in Supplement- 97.6% ID. I dont know where PDBe found their GPcl aa numbers from (they use 32-188 and 509-632-which does bear some resemblance to the Dube-2009 publication numbering. The EBOV GP accession number is given in the Supplement:- KJ660347.1 which corresponds (100% identity) to the sequence in Uniprot entry X5HMX4. The exact sequence of "Primed GPcl" is not given. Dube et al 2009 state that cathepsin cleavage results in the GP protein containing residues 33 to 190 of GP 1 and all residues of GP 2 (502-676). gaof@im.ac.cn 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF. EBOLA protein:-PDBe use the nearest reviewed entry - P87666 which has ~16 aas different from the one quoted in Supplement- 97.6% ID. I dont know where PDBe found their GPcl aa numbers from (they use 32-188 and 509-632-which does bear some resemblance to the Dube-2009 publication numbering. To mimic endosomal protease cleavage and produce primed EBOV GP (GPcl) which was capable of NPC1 binding, EBOV GP-ecto-Delta-mucin protein was incubated with thermolysin. The EBOV GP accession number is given in the Supplement:- KJ660347.1 which corresponds (100% identity) to the sequence in Uniprot entry X5HMX4. The exact sequence of "Primed GPcl" is not given. Dube et al 2009 state that cathepsin cleavage results in the GP protein containing residues 33 to 190 of GP 1 and all residues of GP 2 (502-676). gaof@im.ac.cn Accepted 2017-MAR-08 AT 08:53 GMT AT 08:53 GMT by ORCHARD Cell 2016 imex curation I have added the extra Expt and also altered all the viral constructs so that the TWO "Sufficient to Bind" regions have been amalgamated into ONE - throughout the publication. Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1. Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1. Cell 2016 imex curation Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF. EBOLA protein:-PDBe use the nearest reviewed entry - P87666 which has ~16 aas different from the one quoted in Supplement- 97.6% ID. I dont know where PDBe found their GPcl aa numbers from (they use 32-188 and 509-632-which does bear some resemblance to the Dube-2009 publication numbering. The EBOV GP accession number is given in the Supplement:- KJ660347.1 which corresponds (100% identity) to the sequence in Uniprot entry X5HMX4. The exact sequence of "Primed GPcl" is not given. Dube et al 2009 state that cathepsin cleavage results in the GP protein containing residues 33 to 190 of GP 1 and all residues of GP 2 (502-676). gaof@im.ac.cn 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF., Wang H., Shi Y., Song J., Qi J., Lu G., Yan J., Gao GF. EBOLA protein:-PDBe use the nearest reviewed entry - P87666 which has ~16 aas different from the one quoted in Supplement- 97.6% ID. I dont know where PDBe found their GPcl aa numbers from (they use 32-188 and 509-632-which does bear some resemblance to the Dube-2009 publication numbering. To mimic endosomal protease cleavage and produce primed EBOV GP (GPcl) which was capable of NPC1 binding, EBOV GP-ecto-Delta-mucin protein was incubated with thermolysin. The EBOV GP accession number is given in the Supplement:- KJ660347.1 which corresponds (100% identity) to the sequence in Uniprot entry X5HMX4. The exact sequence of "Primed GPcl" is not given. Dube et al 2009 state that cathepsin cleavage results in the GP protein containing residues 33 to 190 of GP 1 and all residues of GP 2 (502-676). gaof@im.ac.cn Accepted 2017-MAR-08 AT 08:53 GMT AT 08:53 GMT by ORCHARD Cell 2016 imex curation I have added the extra Expt and also altered all the viral constructs so that the TWO "Sufficient to Bind" regions have been amalgamated into ONE - throughout the publication. npc1_human NPC intracellular cholesterol transporter 1 NPC1 Niemann-Pick C1 protein protein protein human Homo sapiens Human MTARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF DIP protein O15118 original sequence version: 157 DA1523E09822E5C7 x5hmx4_9mono GP protein protein Zaire ebolavirus Zaire ebolavirus MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTAVSNGPKNISGQSPARTSSDPETNTTNEDHKIMASENSSAMVQVHSQGRKAAVSHLTTLATISTSPQPPTTKTGPDNSTHNTPVYKLDISEATQVGQHHRRADNDSTASDTPPATTAAGPLKAENTNTSKSADSLDLATTTSPQNYSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRREVIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYTEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDFVDKTLPDQGDNDNWWTGWRQWIPAGIGVTGVIIAVIALFCICKFVF 9FD5F72F401A6069 gp-npc1-3 1 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range X5HMX4:p.Thr42Val mutation mutation certain certain sequence position certain certain certain sequence position certain T V Wed May 02 15:43:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr42val' Tue Jun 30 18:38:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr42Val' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 12 direct interaction direct interaction Table S1 2.3A R free = 0.181 R work = 0.23 gp-npc1-1 2 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range X5HMX4:p.Thr42Val mutation mutation certain certain sequence position certain certain certain sequence position certain T V Wed May 02 15:59:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr42val' Tue Jun 30 15:53:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr42Val' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O15118:p.[Tyr423_Pro424delinsAlaAla;Phe503_Phe504delinsAlaAla;Tyr506Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain FF AA certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain YP AA Wed May 02 17:42:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_pro423-424ala_ala,phe_phe503-504ala_ala,tyr506ala' Tue Jun 30 19:22:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr423_Pro424delinsAlaAla;Phe503_Phe504delinsAlaAla;Tyr506Ala]' O15118:p.Tyr423_Pro424delinsGlyGly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain YP GG Wed May 02 19:00:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_pro423-424gly_gly' Tue Jun 30 15:41:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr423_Pro424delinsGlyGly' O15118:p.Phe503_Phe504delinsGlyGly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain FF GG Wed May 02 16:04:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe_phe503-504gly_gly' Tue Jun 30 20:15:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe503_Phe504delinsGlyGly' O15118:p.Phe503_Phe504delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain FF AA Wed May 02 17:03:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe_phe503-504ala_ala' Tue Jun 30 19:52:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe503_Phe504delinsAlaAla' O15118:p.Phe503Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F G Wed May 02 18:15:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe503gly' Tue Jun 30 19:17:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe503Gly' O15118:p.Phe504Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F G Wed May 02 19:19:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe504gly' Tue Jun 30 20:15:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe504Gly' O15118:p.Phe503Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 18:05:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe503ala' Tue Jun 30 18:45:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe503Ala' O15118:p.Phe504Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:27:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe504ala' Tue Jun 30 17:58:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe504Ala' O15118:p.Tyr506Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Mon Feb 27 13:58:09 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'phe506ala' Wed May 02 18:17:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr506ala' Tue Jun 30 16:29:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr506Ala' O15118:p.Pro424Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P G Wed May 02 17:32:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro424gly' Tue Jun 30 19:48:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro424Gly' O15118:p.Tyr423Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:45:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr423ala' Tue Jun 30 19:12:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr423Ala' O15118:p.Pro424Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P A Wed May 02 18:14:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro424ala' Tue Jun 30 16:49:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro424Ala' ecoli Escherichia coli (strain K12) 21 17 direct interaction direct interaction Fig 2 B/C, fig 6 gp-npc1-2 2 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range region sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range X5HMX4:p.Thr42Val mutation mutation certain certain sequence position certain certain certain sequence position certain T V Wed May 02 19:48:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr42val' Tue Jun 30 18:46:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr42Val' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region glycores glycosylated residue GlycoRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NGFR signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 43 38 43 39 direct interaction direct interaction Fig 2 E/F gp-1 3 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range X5HMX4:p.Thr42Val mutation mutation certain certain sequence position certain certain certain sequence position certain T V Wed May 02 16:52:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr42val' Tue Jun 30 19:56:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr42Val' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supp 4A