DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure (London, England : 1993) 2016 imex curation Hänzelmann P., Schindelin H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hänzelmann P., Schindelin H. DIP original experiment: DIP-268472X Structure (London, England : 1993) 2016 IMEx Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure (London, England : 1993) 2016 imex curation Hänzelmann P., Schindelin H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hänzelmann P., Schindelin H. DIP original experiment: DIP-268470X Structure (London, England : 1993) 2016 IMEx Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure (London, England : 1993) 2016 imex curation Hänzelmann P., Schindelin H. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Hänzelmann P., Schindelin H. DIP original experiment: DIP-268475X Structure (London, England : 1993) 2016 IMEx Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure (London, England : 1993) 2016 imex curation Hänzelmann P., Schindelin H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hänzelmann P., Schindelin H. DIP original experiment: DIP-268473X Structure (London, England : 1993) 2016 IMEx Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure (London, England : 1993) 2016 imex curation Hänzelmann P., Schindelin H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hänzelmann P., Schindelin H. DIP original experiment: DIP-268471X Structure (London, England : 1993) 2016 IMEx Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure (London, England : 1993) 2016 imex curation Hänzelmann P., Schindelin H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hänzelmann P., Schindelin H. DIP original experiment: DIP-268474X Structure (London, England : 1993) 2016 IMEx Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein P55072 original sequence version: 89 DIP protein P55072 original sequence version: 114 DIP protein P55072 original sequence version: 123 DIP protein P55072 original sequence version: 150 DIP protein P55072 original sequence version: 174 DIP protein P55072 original sequence version: 175 DIP protein P55072 original sequence version: 176 DIP protein P55072 original sequence version: 160 DIP protein P55072 original sequence version: 128 DIP protein P55072 original sequence version: 132 501B721D3A77BA8A o95155-1 Ubiquitin conjugation factor E4 B UBE4B HDNB1 KIAA0684 UFD2 Homozygously deleted in neuroblastoma 1 RING-type E3 ubiquitin transferase E4 B Ubiquitin fusion degradation protein 2 protein protein human Homo sapiens Human MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSPAPTPSFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYTVTHPWASSGVSILSSSPSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH DIP protein O95155-1 original sequence version: 88 DIP protein O95155 original sequence version: 138 ufd1_human Ubiquitin recognition factor in ER-associated degradation protein 1 UFD1 UFD1L Ubiquitin fusion degradation protein 1 protein protein human Homo sapiens Human MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAGELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFVAFSGEGQSLRKKGRKP DIP protein Q92890 original sequence version: 91 DIP protein Q92890 original sequence version: 134 FC69860002C042C6 amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS DIP protein Q9UKV5 original sequence version: 136 DIP protein Q9UKV5 original sequence version: 122 DIP protein Q9UKV5 original sequence version: 113 DIP protein Q9UKV5 original sequence version: 85 DIP protein Q9UKV5 original sequence version: 103 DIP protein Q9UKV5 original sequence version: 40 8782324609C0E62A nsf1c_human NSFL1 cofactor p47 p97 cofactor p47 NSFL1C UBXN2C UBX domain-containing protein 2C protein protein human Homo sapiens Human MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT DIP protein Q9UNZ2 original sequence version: 104 DIP protein Q9UNZ2 original sequence version: 145 DIP protein Q9UNZ2 original sequence version: 112 79364617F940B9F9 ufd1l-vcp-2 2 9 unspecified role unspecified role unspecified role unspecified role Region 221-241 Region 221-241 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1-708/729-806 Region 1-708/729-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nsfl1c-vcp-15 3 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 1-480 Region 1-480 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P55072:p.Arg113Ala R113A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R113A'. Wed May 02 15:47:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg113ala' Tue Jun 30 16:37:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg113Ala' P55072:p.His183Ala H183A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H183A'. Wed May 02 17:36:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his183ala' Tue Jun 30 19:42:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His183Ala' P55072:p.Phe131Ala F131A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F131A'. Wed May 02 19:29:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe131ala' Tue Jun 30 18:03:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe131Ala' ecolx Escherichia coli direct interaction direct interaction ube4b-vcp-4 4 8 unspecified role unspecified role bait bait Region 1-23 Region 1-23 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey Region 1-480 Region 1-480 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P55072:p.Asp35Ala D35A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D35A'. Wed May 02 18:36:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp35ala' Tue Jun 30 19:55:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp35Ala' P55072:p.Asp35Asn D35N mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D N This feature has been corrected as a result of our quality control procedures. The original label was 'D35N'. Wed May 02 17:08:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp35asn' Tue Jun 30 17:50:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp35Asn' P55072:p.Val38Ala V38A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V38A'. Wed May 02 19:50:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val38ala' Tue Jun 30 19:53:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val38Ala' P55072:p.Ile70Ala I70A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I70A'. Wed May 02 19:07:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile70ala' Tue Jun 30 16:21:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile70Ala' P55072:p.Leu72Ala L72A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L72A'. Wed May 02 16:56:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu72ala' Tue Jun 30 20:21:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu72Ala' P55072:p.Tyr143Ala Y143A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y143A'. Wed May 02 20:22:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr143ala' Tue Jun 30 19:54:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr143Ala' P55072:p.Ile175Ala I175A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I175A'. Wed May 02 18:36:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile175ala' Tue Jun 30 20:03:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile175Ala' P55072:p.Phe52Ala F52A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F52A'. Wed May 02 15:23:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe52ala' Tue Jun 30 18:01:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe52Ala' P55072:p.Arg53Ala R53A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R53A'. Wed May 02 16:48:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg53ala' Tue Jun 30 19:40:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg53Ala' ecolx Escherichia coli direct interaction direct interaction amfr-vcp-1 1 10 unspecified role unspecified role bait bait Region 622-640 Region 622-640 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 7 unspecified role unspecified role prey prey Region 1-480 Region 1-480 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P55072:p.Asp35Ala D35A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D35A'. Wed May 02 19:55:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp35ala' Tue Jun 30 16:03:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp35Ala' P55072:p.Asp35Asn D35N mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D N This feature has been corrected as a result of our quality control procedures. The original label was 'D35N'. Wed May 02 19:46:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp35asn' Tue Jun 30 16:31:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp35Asn' P55072:p.Val38Ala V38A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V38A'. Wed May 02 17:44:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val38ala' Tue Jun 30 16:57:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val38Ala' P55072:p.Ile70Ala I70A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I70A'. Wed May 02 15:38:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile70ala' Tue Jun 30 17:31:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile70Ala' P55072:p.Leu72Ala L72A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L72A'. Wed May 02 19:25:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu72ala' Tue Jun 30 17:08:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu72Ala' P55072:p.Tyr143Ala Y143A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y143A'. Wed May 02 17:26:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr143ala' Tue Jun 30 17:17:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr143Ala' P55072:p.Ile175Ala I175A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I175A'. Wed May 02 18:15:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile175ala' Tue Jun 30 19:31:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile175Ala' ecolx Escherichia coli direct interaction direct interaction ufd1l-vcp-3 5 9 unspecified role unspecified role bait bait Region 221-241 Region 221-241 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 7 unspecified role unspecified role prey prey Region 1-480 Region 1-480 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P55072:p.Arg113Ala R113A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R113A'. Wed May 02 14:44:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg113ala' Tue Jun 30 16:15:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg113Ala' P55072:p.His115Ala H115A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H115A'. Wed May 02 19:48:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his115ala' Tue Jun 30 16:53:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His115Ala' P55072:p.His183Ala H183A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H183A'. Wed May 02 14:27:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his183ala' Tue Jun 30 19:23:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His183Ala' P55072:p.Phe131Ala F131A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F131A'. Wed May 02 18:00:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe131ala' Tue Jun 30 16:20:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe131Ala' ecolx Escherichia coli direct interaction direct interaction ufd1l-vcp-4 6 9 unspecified role unspecified role bait bait Region 221-241 Region 221-241 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q92890:p.Phe225Ala F225A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F225A'. Wed May 02 19:15:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe225ala' Tue Jun 30 16:25:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe225Ala' Q92890:p.Asn233Ala N233A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N233A'. Wed May 02 19:07:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn233ala' Tue Jun 30 15:43:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn233Ala' Q92890:p.Arg234Ala R234A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R234A'. Wed May 02 19:24:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg234ala' Tue Jun 30 16:04:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg234Ala' Q92890:p.Leu235Ala L235A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L235A'. Wed May 02 17:48:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu235ala' Tue Jun 30 20:00:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu235Ala' Q92890:p.Phe228Ala F228A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F228A'. Wed May 02 20:16:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe228ala' Tue Jun 30 19:53:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe228Ala' in vitro In vitro 7 unspecified role unspecified role prey prey Region 1-480 Region 1-480 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction