DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Structure (London, England : 1993) 2016 imex curation Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. Only protein-protein interactions ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. DIP original experiment: DIP-268464X Structure (London, England : 1993) 2016 IMEx Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Structure (London, England : 1993) 2016 imex curation Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. DIP original experiment: DIP-268463X Structure (London, England : 1993) 2016 IMEx Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Structure (London, England : 1993) 2016 imex curation Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. DIP original experiment: DIP-268465X Structure (London, England : 1993) 2016 IMEx Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Structure (London, England : 1993) 2016 imex curation Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. DIP original experiment: DIP-268462X Structure (London, England : 1993) 2016 IMEx Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Structure (London, England : 1993) 2016 imex curation Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. DIP original experiment: DIP-268467X Structure (London, England : 1993) 2016 IMEx Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. Structure (London, England : 1993) 2016 imex curation Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nie S., Ke H., Gao F., Ren J., Wang M., Huo L., Gong W., Feng W. DIP original experiment: DIP-268466X Structure (London, England : 1993) 2016 IMEx Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators. q8bjs4-3 SUN domain-containing protein 2 Sun2 Unc84b Protein unc-84 homolog B Sad1/unc-84 protein-like 2 protein protein mouse Mus musculus Mouse MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSYYSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFGSSSGYSSEDDLAGRLFGLLYWWIGTTWYRLTTAASLLDVFVLTRSRHFSLNLKSFLWFLLLLLLLTGLTYGAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDASQHFQAEQRVLSRVHSLERRLEALAADFSSNWQKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLVSRREATLKEDLRRDTVAHIQEELATLRAEHHQDSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQESSVKELGRLEAQLASLRQELAALTLKQNSVADEVGLLPQKIQAARADVESQFPDWIRQFLLGDRGARSGLLQRDEMHAQLQELENKILTKMAEMQGKSAREAAASLGQILQKEGIVGVTEEQVHRIVKQALQRYSEDRIGMVDYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGTFAYDQDGEPIQTFYFQASKMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH DIP protein Q8BJS4-3 original sequence version: 98 No experimental confirmation available. q6zwq0-1 Nesprin-2 Syne2 KASH domain-containing protein 2 Nuclear envelope spectrin repeat protein 2 Nucleus and actin connecting element protein Synaptic nuclear envelope protein 2 NUANCE Nesp2G Nesprin-2 Giant protein protein mouse Mus musculus Mouse MAASPVLPTEDGEGFLGIDDLHFSLQAEQEDTQKKTFTCWINSQLAKHTPPSVVSDLFADIKKGHVLLDLLEVLSGQQLPRDKGSNTFQCRINIEHALTFLKNRSIKLINIHVADIVEGNPSIILGLIWTIILHFHIEKLAQTLSCDYNQPSPEVVSVAASSPTSSPPTKKCSKAQAQARWQWSAKKALLQWAQEQCARSESVNVTDFKSSWRNGMAFLAVIHALRPDLIDMDSMRHRSNKDNLKEAFRIAEHELKIPKLLEPEDVDVVNPDEKSIMTYVAQFLKYSKDAPGPGDSTQAKVRDALVWLTLQEKRFQKMLKDSASETYCNKYHSLLSFMESLNEEKESFIDVLSLKGRMGELNEDESRLRQGWTSLMHQVAAWRAQLDDALPSPLKETEAWLKDIEGVVQEGVPTSQSYSEARTLIQGKLSSFKSLMGSFDYHSDVLMAFQSNAEKSLPAVPPVKLEEMTRRINNVLGKNFIPLLEFHDSKCSVLALLDEAKAKLDVWNGTYESKESVEVLLEDWHKFTGEKKFLIQLDASFQKCEEMYKNSARECESIREEYMMLEKNVHSCRQYIHNTKATLQRALMSWATFEEDLALLKASFDLTKKEQIKEVPVETLLQWNTKHTSLNEVGSFLIGVSSREVAASISKELRRLNKRWRKFITKTPLLKLPLVKIQDQPPGNSSGTSLSKESAMAAEPGGSRGEDVKAAEKQEVEDEESAGQLKVNEEVEGLIKQVTIWESQTKSILDLLQHGDHADGSSADTLQHLIAKGSVYEELLARTEDTLQMDVQSPSNLEPFQNVLRAGLQAKIQEAKQGVQITMVELSAVLKNLSDEPLELDLGLKVEEAQKELEVSILRAEQLLGQRERPGGFLLKYKEALEILNTNSLAKYLRAVEELKRTVPGGAKLQLEEQSRVASAKWEPLRHEISLYLQQLKIAIEEEKLRDNIARLEKQINKEKKLIRRGRTRGLRKEHEACLSPESIKCQLEHHVGVLRVLCEELTSPEDQQELKRALRDYEQKIARLLKCASEIHTTLQSSQGGALEERSALITTENGRRDADGEVPLEIPDNQLSTEKAMEPIKNFSQTSELKPQQEESIMEKEGKDCSASLSDLQERYDTQRGLLEQHLQDSKSRVTSDFASEQERSSACLQSKLAELQVLLADTDAHWEKFEITSLNLRRLMSDAEKPVLNQERDLLKGNEQVLHGLLNTRMESLEMALQIVLPLEKECSLLCASDLPLCTVAVQDLHPVEIDGVYQNLRDIRDSIAKQIRVCTSLEEPSNSVPRELHTLDQCAIQDIVLKCRLQLETMNQKVEMREDALDALEGFLASLRAAKLSAELPADRPAPKAPEVLSEDILLMKEKAGPLDERLRTLGINIKDAEGGENTTCERLVGALSVNLVAMDGQSKEEGPPEDKKLLEACSSKNLELFKNIQDLQNQISKIGLKDPTAPAVKHRKKSLLRLDKDLDGLEEEKVRIQKIAGSLPRFKDGSEKNVIQQCEDTAALWESTKASVTESLEQCGSALELLRQYQNIKNNLTALIQKEEGIISQQASYMGKDNLKKKIAEIETVKEEFSDHLEVVDKINQICKNLQYHLNKMKTFEDPPFEKEANAIVDRWLDINEKTEEYGENLGRALALWDKLFIIKNNIDEWTEQILGKAESHELTEEDRGRLKEELKVLEEQSAEFSRRVADIQSLLQSNEKPLELQVMESSVLSKMKDVKTHVAGGSNSYAPSGSTAELREDLDQAKTQMGMTESLLNALSPSDSLEIFTKLEEIQQQIFQQKHSMTVLENQIGCLTPELSELKRQYASVSNLFNTKKNALQDHFATFLNEQCKNFNDWFSNVKTNLQECFEPPETKLSLEQRLQKLSDFLTLGGGNSKIQQVETVLQHVKMLLPKAHVKELDSWLRSQELELENMESICQARAGELNNSFQQLLRLEDDCRSLSKWLTNQEENWGKMEVSGERMDLFSQALTRKREQFETVAQLSDSLKEHGLTEGEETIKESTHLIDRYQALWRQLHEIEEEDKLPAAEDQSFNDLADDVIHWIKEIKESLMALNSSEGKMPLEERIQKIKEIIALKPEGDAKIQMVMRQAEHCEAPLAQETFTDLSNQWDSTLHLANTYLSHQEKLVLEGEKYLQSKEDLRLMLTELKKQQEAGFALQPGLPEKQAQLKIYKKFLQKAQDLTSLLEELKSQGNYLLECTKNPSFSEEPWLEVKHLHESLLQQLQDSVQKLEGHVQEHSSYQVCLTDLSSTLDDISKEYFSLCDGSKDQIMAKERMQKLQELESRLRFQGGALKKASALAKSIKQNTSSVGQKIIKDDIRSLKYKQKDLENRIESAKQETENGLNSILKSKSSTEKHVKFSLPVEEMPATSEVPKPTRESAAVGESGGARETNTNSAVEMILSKQLSLNVQESMQNAQDEREVNELQNQPLELDIMLRNEQLKGMEELSTHLEARRAAIELLEQSQHLNQTEEQALVLPAARPSVCHLGSLQQELHTLKKTKERQYGLLSGFQDQLVMAEASLNTSLAEVESLKIGSLDSATYLGKIKKFLGSVENQQGSLSKLRTEWAHLSSLLAAADQKLVESQMKHLEHGWELVEQLAHRKCFQQATEHSELTCLLEKLQDLKVSLHQQQQRLTLSLNSPGQQAAIVDMVTPAAELQAIKCEFSGLKWQAELHMKRLWGEKDKKTLEDAINNLNKQMEALEPLNREVENRIKKCELQNRIKETLSWVKNTMAELVVPIALLPDNILSQIRKCKLIHDGILGNQQAVELLVEEVRGITPSLAPCEGDGLNALLEDLQSQHQALLLKSTERSQQLELKLEEKSKLFAIIGKVQLTLEESETLMSPTGDRASTEAELERRLAILKASQQQLQDTESALSAHLQELTNAYKDANVFERLFLDDQLKNLKARTNRTQRFLQNNGSELKQKMESYREFHDKAAVLQKEAECILHGGLLPLRQELEQDAKEQLGNLRDKLAAIRGSLSQVLTSEEVFDTIGLSWDGSLLARLQTQVLEREREVEGKIKQLDTFLIARDRHQASISKIRAVDLQIKKGAESLLKVPSMSPESTLLNAQTLIQKIEKSKRLRDEIIRKLSKNEAFDDSFKESEMQRLKLCAEENSRLQEALQNMLLELQPREMGEKEFREKLENALHVLKQIQSRLQQPLCVNLGVQHIQHEKETWEAFGEQVEAEMCGLRAVRITEEQREENDSGTGGMEAKLRDIEGLHMELSKSISLRADVLNDAYDSANRYDELVAGALRIITSLEATLLSYRVDLHNPQKTLELAHLKQEELQSSVADLRSLTETLGAISSPEAKEQLRCTLEVLAAKNSALKAGLEAQEAEEERCLENYKCFRKMKEEICSRLRKMEMDLGQSIFPLPRSYKEALARLEQSKALTSNLLSTKEDLVKLRQLLRHLRCRSTENDATCALGVASALWEKWLSLLEAAREWQQWGGELKREWKFISEEIEREAIILETLQEDLPEISKTNAAPTEELWQLLDSLCQHQESVEKQQLLLALLLQRVRSIQNIPEGTETGETIPALQEIGSMQERCDRLLHTTRKNKDLVQAEIQAQQSFLKEIKDVKRVFEQISTSFPNLAPEGHPERAEQFEELRSILQKGKLSFENIMEKLRIKYSEMYSIVPAEIGSQVEECRSALEDAEEKMSSEVSKSSPSSIMRRKIERINNGLHCVEKMLQQKSRNIEEAHEIQKKIWDELDLWHSKLNELDSEVQDFVEQDPGQAQEWMDNLMAPFQQHQQVSQRAESRTSQLNKATIKMEEYNDLLKSTEVWIEKTSCLLANPACYDSSRTLSHRASTLQMALEDSEQKHSLLHSIFTDLEDLSIIFETDDLIQTIHELSDQVAALQQQIMEALPHVQQVADDVVAIESEVKAMEKKVAKIKAILLSKEIFDFPPEEHLKHGEVILENIHPMKKTIAEIMTYQVELRLPQTGTKPLPVFQRTSQLLQDVKLLENVTQEQNELLKVVIKQTAECDEEIDSLKQMLTNYSAEISPEHVSQNQVADLPSLQGEMERLEKQILNLNQKKEDLLVDLKTAVLNLHEHLKQEQQEVGDKPSAGASECTVAERDASERKLSRTNSMSFLPVVKEEAEESSVKSEDGRRRTEPPSASWSFLGKHSKDLEGDGASSSSSATIVQDADGRISTCDSSMVHIIAPDSGSTEEGPAPSPRLSQTDEGATPPIEAALLDFPREQGAFESTVERSRPRPADILRVCKTQVAKLELWLQQANVAFEPETVDADMQQVVEEELAGCQAMLTEIEYKVASLLETCKDQGLGDCGTTQQEAEALSWKLKTVKCNLEKVQMVLQEKFSEDQHPSTLKKPSEPHDVDQPAGLSELDSVLTERPQFSRQKDAPQPQILELKPSEQKDLIKFTELNAKKTWLQGHQENEDANRQSASSSKVPSPGNAASDSTLPLQAQSGDKWQYLHHELTSRPNPSVPQLVEPQVALTTSTLPSVSVYNFRCPTADELQAYTTQLEELRQEANTIQTQGSMTEETYISLDKRLFELFLSLSRCLGSVEGLLQRPGLLREDACAQQVFFQKLALELKKLYLALGDKKDDFLKAVTWPGKEATLLPECIDALTVSLESVQSRAAWRDASLKAGLEHSRSYQNEVKRLYSQLIKKKTALQQSLNEISGQSISKQLQKADVHTAELQNSEKQVAKLRDEGERLRFPHGLLQDVYKLEDVLDSMWGILRARYLELSSPFLSKSLQTLLQGMAELVSIGKGKLAADPLQHAKSKAALQAQLQDHKAFFQKLVADMLLIQTYSATMFPPSLQKGEGFGAEQVAEVRALEEEACLRGAQLQSMLQKWEEFDDNYASLEKDLEALISSLPSVSLVEETEERLLERISFYQQIKRNIDGKHARLCQTLNEGRQLAASVSCPEPEGQIARLEEQWLSLNKRIDQELHRLQTLLKHLLSYSRDSDELTRWLETSQQTLNYWKEQSLNVSQDLNTIRSNIDRFFKFSKEVDERSSLKSAVMSTGNQLLHLKEADTATLRASLAQFEQKWTVLITQLPDIQEKLHQLQMEKLPSREAISEMISWMNAVEPQAAGKDTELSKSSASQVKHLLQKLKEFRMEMDYKQWVVDFVNQSLLQLSTCDVESKRYERTEFAEHLGEMNRQWQRVHGTLNRKIQHLEQLLESITENENKVQNLNSWLEAQEERLKMLQKPESAVSMEKLLLDCQDIENQLALKSKALDELRQSSLTMDGGDVPLLEDMASGIVELFQKKNNVTSQVHQLRASVQSVLQEWKACDKLYDEATMRTTQLTYSMEHSKPAVLSLQALACQVQNLEALQDEAENGERSWEKLQEVIGRLKASCPSMAGIIEEKCQDAHSRWTQVNQDLADQLQEARGQLQLWKAPHNAHAEAAAWLQQQEAKFQQLANTNLSGDNLADILPRALKDIKGLQSDLQKTKEAFLENSTLSDQLPQPEERSTPGLHSGQRHSLQTAAYLEKMLLAKSNEFEIVLAQFKDFTDRLAYSKDLIVHKEENLNKLYHEEKEEVPDLFLNHVLALTAQSPDIERLNEESLRLPLSDVTIKTLQSLNRQWIRATATALDHYSELQGNGLNEKFLHYCERWIQVLEKIQESLSVEVAHSLPALLEQQKTYEILEAEVSTNQAVADAYVTQSLQLLDTAEIEKRPEFVSEFSKLSDQWQRAARGVRQRKCDISRLVTQWRFFTTSVEDLLRFLADTSQLLSAVKEQDCYSLCQTRRLVHELKSKEIHLQRWRTTYALALEAGEKLRNTPSPETREFVDGQISRLQESWKDTELSLGEVISRLQSTAETWDQCKKKIKKLKKRLQALKAQSEDPLPELHEALHEEKELIKEVEKSLANWTHSLKELQTMKADLSQHILAEDVTVLKEQIQLLHRQWEDLCLRVAIRKQEIEDRLNSWIVFNEKNKELCAWLVQMENKVLQTADVSIEEMIEKLQKDCMEEISLFTENKLQLKQMGDQLIKASSKAKAAELEEKLSKINDRWQHLFDVIGSRVKKLKETFAFIQQLDKNMSNLRTWLARIESELSKPVVYDVCDNQEIQKRLAEQQDLQRDIEQHSAGVESVFNICDVLLHDSDACANETECDSIQQTTRSLDRRWRNICAMSMERRMKIEETWRLWQKFLDDYSRFEDWLKSAERTAACPNSSEVLYTNAKEELKRFEAFQRQIHERLTQLELINKQYRRLARENRTDTASKLKQMVHEGNQRWDNLQKRVTAILRRLRYFTNQREEFEGTRESILVWLTEMDLQLTNVEHFSESDAEDKMRQLNGFQQEITLNTNKIDQLIVFGEQLIQKSEPLDAVLIEDELEELHRYCQEVFGRVSRFHRRLTSHTPGLDDEKEASENETDIEDPREIQADSWRKRRESEEPTSPQSLCHLVPPALGHERSGCETPVSVDSIPLEWDHTGDVGGSSSHEDDEEGPFYSALSGKSISEGHPWHVPDSPSHSKHHYKHMEGDRTEAPVPTDASTPFKSDYVKLLLRQGTDDSKEGLKEAQQEDEQLATLTGQQPGAFDRWELIQAQELHSKLRLKQTVQQLKSDIGSIAAWLGKTEAELEALKLAEPPSDIQEIALRVKRLQEILKAFDTYKALMVSVNVSHKEYLPSQSPEATELQNRLHQLSLSWDSVQGVLDSWRGDLRQSLMQCQDFHQLSQDLLLWLATAESRRQKAHVTSPEADRQVLLECQKDLMRLEKELVARQPQVSSLREISSSLLVKGQGEDYIEAEEKVHVIEKKLKQLQEQVAQDLMSLQRSLDPDASLTSFDEVDSGEQLPAAFAKSPRPRWTFLEEEEEEEETDSRMPHLDSPGSSQPRRSFLSRVIRAALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT DIP protein Q6ZWQ0-1 original sequence version: 87 DIP protein Q6ZWQ0-1 original sequence version: 100 syne2-sun2-4 3 8 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 6799-6874 Region 6799-6874 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role prey prey Region 378-699 Region 378-699 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8BJS4-3:p.[Leu400Ala;Leu403Ala] L400A/L403A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L400A/L403A'. Wed May 02 20:30:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu400ala,leu403ala' Tue Jun 30 16:31:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu400Ala;Leu403Ala]' ecolx Escherichia coli direct interaction direct interaction sun2-7 4 7 unspecified role unspecified role unspecified role unspecified role Region 378-450 Region 378-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8BJS4-3:p.[Leu400Ala;Leu403Ala] L400A/L403A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L400A/L403A'. Wed May 02 18:08:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu400ala,leu403ala' Tue Jun 30 16:51:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu400Ala;Leu403Ala]' ecolx Escherichia coli direct interaction direct interaction sun2-8 5 7 unspecified role unspecified role unspecified role unspecified role Region 378-450 Region 378-450 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sun2-3 2 7 unspecified role unspecified role unspecified role unspecified role Region 378-450 Region 378-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sun2-syne2-2 6 8 unspecified role unspecified role prey prey Region 6799-6874 Region 6799-6874 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait Region 378-699 Region 378-699 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8BJS4-3:p.[Leu400Ala;Leu403Ala] L400A/L403A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L400A/L403A'. Wed May 02 15:44:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu400ala,leu403ala' Tue Jun 30 18:20:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu400Ala;Leu403Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association sun2-4 1 7 unspecified role unspecified role unspecified role unspecified role Region 378-450 Region 378-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction