DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Structure (London, England : 1993) 2015 imex curation Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. DIP original experiment: DIP-267826X Structure (London, England : 1993) 2015 IMEx Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Structure (London, England : 1993) 2015 imex curation Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. DIP original experiment: DIP-267824X Structure (London, England : 1993) 2015 IMEx Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Structure (London, England : 1993) 2015 imex curation Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. DIP original experiment: DIP-267827X Structure (London, England : 1993) 2015 IMEx Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Structure (London, England : 1993) 2015 imex curation Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. DIP original experiment: DIP-267823X Structure (London, England : 1993) 2015 IMEx Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. Structure (London, England : 1993) 2015 imex curation Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Helander S., Montecchio M., Pilstål R., Su Y., Kuruvilla J., Elvén M., Ziauddin JM., Anandapadamanaban M., Cristobal S., Lundström P., Sears RC., Wallner B., Sunnerhagen M. DIP original experiment: DIP-267825X Structure (London, England : 1993) 2015 IMEx Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity. pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q13526 original sequence version: 65 DIP protein Q13526 original sequence version: 64 DIP protein Q13526 original sequence version: 163 DIP protein Q13526 original sequence version: 156 DIP protein Q13526 original sequence version: 117 35391AF40B7D1E13 myc_mouse Myc proto-oncogene protein Proto-oncogene c-Myc Transcription factor p64 Myc protein protein mouse Mus musculus Mouse MPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA DIP protein P01108 original sequence version: 146 DIP protein P01108 original sequence version: 167 3FCA39BFFD6FC59E myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA DIP protein P01106 original sequence version: 195 DIP protein P01106 original sequence version: 196 DIP protein P01106 original sequence version: 148 DIP protein P01106-1 original sequence version: 161 DIP protein P01106 original sequence version: 182 DIP protein P01106 original sequence version: 197 DIP protein P01106-1 original sequence version: 208 DIP protein P01106 original sequence version: 121 DIP protein P01106-1 original sequence version: 144 DIP protein P01106 original sequence version: 144 ED5C028029A4C5D1 myc-pin1-1 4 8 unspecified role unspecified role bait bait Region 1-88 Region 1-88 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 6-39 Region 6-39 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 50-163 Region 50-163 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction myc-pin1-2 5 8 unspecified role unspecified role unspecified role unspecified role Region 1-88 Region 1-88 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction myc-pin1-3 3 8 unspecified role unspecified role bait bait Region 1-88 Region 1-88 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P01106:p.[Pro21Ala;Tyr22Ala;Phe23Ala;Tyr24Ala] P21A/Y22A/F23A/Y24A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P21A/Y22A/F23A/Y24A'. Wed May 02 15:48:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro21ala,tyr22ala,phe23ala,tyr24ala' Tue Jun 30 17:09:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro21Ala;Tyr22Ala;Phe23Ala;Tyr24Ala]' P01106:p.[Pro21Ala;Tyr22Ala] P21A/Y22A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P21A/Y22A'. Wed May 02 14:47:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro21ala,tyr22ala' Tue Jun 30 17:04:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro21Ala;Tyr22Ala]' P01106:p.[Tyr22Ala;Phe23Ala] Y22A/F23A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y22A/F23A'. Wed May 02 15:19:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr22ala,phe23ala' Tue Jun 30 17:06:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr22Ala;Phe23Ala]' ecolx Escherichia coli 6 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction myc-pin1-4 2 8 unspecified role unspecified role unspecified role unspecified role Region 1-88 Region 1-88 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 6-39 Region 6-39 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 50-163 Region 50-163 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction myc-pin1-5 1 6 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 7 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P01108:p.[Tyr22Ala;Phe23Ala;Ile24Ala] Y22A/F23A/I24A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y22A/F23A/I24A'. Wed May 02 15:38:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr22ala,phe23ala,ile24ala' Tue Jun 30 18:54:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr22Ala;Phe23Ala;Ile24Ala]' P01108:p.[Tyr22Ala;Phe23Ala] Y22A/F23A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y22A/F23A'. Wed May 02 16:19:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr22ala,phe23ala' Tue Jun 30 17:32:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr22Ala;Phe23Ala]' P01108:p.[Pro21Ala;Tyr22Ala;Phe23Ala] P21A/Y22A/F23A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P21A/Y22A/F23A'. Wed May 02 18:41:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro21ala,tyr22ala,phe23ala' Tue Jun 30 19:11:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro21Ala;Tyr22Ala;Phe23Ala]' human Homo sapiens Human physical association physical association