UniProt UniProt http://www.uniprot.org Binding of the Extracellular Eight-Cysteine Motif of Opy2 to the Putative Osmosensor Msb2 Is Essential for Activation of the Yeast High-Osmolarity Glycerol Pathway. Binding of the Extracellular Eight-Cysteine Motif of Opy2 to the Putative Osmosensor Msb2 Is Essential for Activation of the Yeast High-Osmolarity Glycerol Pathway. Molecular and cellular biology 2015 imex curation Yamamoto K., Tatebayashi K., Saito H. tategone@ims.u-tokyo.ac.jp, h-saito@ims.u-tokyo.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yamamoto K., Tatebayashi K., Saito H. I can't think of a sensible way of entering Fig. 6F and part of 6E not made clearer in another expt, as its all double mutants and I have no idea which has the effect, so i've ignored it unless you have a better idea. tategone@ims.u-tokyo.ac.jp, h-saito@ims.u-tokyo.ac.jp Accepted 2017-NOV-06 AT 11:05 GMT AT 11:05 GMT by MGT Molecular and cellular biology 2015 imex curation opy2_yeast Protein OPY2 OPY2 Overproduction-induced pheromone-resistant protein 2 YPR075C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSSKASASSSLSSTATSSTSATRGSDGCVVCDSTASCPVCASGEYCVMTSLTCDKCPSTYCAKQSDSQLSSLSSSSSSSSSSNSNEKTSLIVGFTVGIVGGAMLIALVALYFINKRYWKPKRQKNKALKLEEASQSYGNEEEYFDDEDDDDEDDEDDGGMRKDESHTLFNTSLVPPTLNVPGNRSSASTTRTRASNILPIAYIPGVTSGLSTDKLQSKLRSSSKRQNAAGDIRSHITLGSSILDGLDDEDDEHNQVLNKDADDNLITAIRAKPKLVQIAEEESDKEIQDLDVIEEQTEADDLSHMAKSEASHGNNDEDDDEEGSFILDLEIPESIRESTQGSRTESPFEDKFEIHDER 6D444AFA25042FFB msb2_yeast Signaling mucin MSB2 MSB2 Multicopy suppressor of bud emergence 2 Osmosensor MSB2 YGR014W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQFPFACLLSTLVISGSLARASPFDFIFGNGTQQAQSQSESQGQVSFTNEASQDSSTTSLVTAYSQGVHSHQSATIVSATISSLPSTWYDASSTSQTSVSYASQESDYAVNQNSWSASTNQLPSTSTTSYYAPTFSTSADFAASSVNAASDVSTASVPIDTSANSIPFTTTSNIETTTSAPLTSDTPLISTSTMSAADNVFSSANPISASLTTTDSSESFDQTSTAGAIPVQSSADFSSSSEILVQSSADFSSPSSPTTTDISLSAAPLQTSESSSFTTASAALPVSSTDVDGSSASPVVSMSAAGQIASSSSTDNPTMSETFSLTSTEVDGSDVSSTVSALLSAPFLQTSTSNSFSIVSPSVSFVPSQSSSDVASSSTANVVSSSFSDIPPQTSTSGSVVSVAQSASALAFQSSTEVYGASASSTMSSLLSTTSLQSTTLDSSSLASSSASSSDLTDYGVSSTASIPLLSASEQASTSSSFSVVSPSVSFVPSQSSSDVASTSAPSVVSSSFSYTSLQAGGSSMTNPSSSTIVYSSSTGSSEESAASTASATLSGSSSTYMAGNLQSQPPSTSSLLSESQATSTSAVLASSSVSTTSPYTTAGGASTEASSLISSTSAETSQVSYSQSTTALQTSSFASSSTTEGSETSSQGFSTSSVLVQMPSSISSEFSPSQTTTQMNSASSSSQYTISSTGILSQVSDTSVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTSVPSTSSRSSVSQVSDTPVPSTSSRSSVSQTSSSLQPTTTSSQRFTISTHGALSESSSVSQQASEITSSINATASEYHSIQTTAATQSTTLSFTDANSSSASAPLEVATSTPTPSSKASSLLLTPSTSSLSQVATNTNVQTSLTTESTTVLEPSTTNSSSTFSLVTSSDNNWWIPTELITQAPEAASTASSTVGGTQTMTLPHAIAAATQVPEPEGYTLITIGFKKALNYEFVVSEPKSSAQIFGYLPEALNTPFKNVFTNITVLQIVPLQDDSLNYLVSVAEVYFPTAEIEELSNLITNSSSAFYTDGMGTAKSMAAMVDSSIPLTGLLHDSNSNSGGSSDGSSSSNSNSGSSGSGSNSNSGVSSSSGNSYQDAGTLEYSSKSNSNVSTSSKSKKKIIGLVIGVVVGGCLYILFMIFAFKYIIRRRIQSQEIIKNPEISSISSSEFGGEKNYNNEKRMSVQESITQSMRIQNWMDDSYYGHGLTNNDSTPTRHNTSSSIPKISRPIASQNSLGWNEV 67D5D984D5CA4A6D msb2-opy2-6 1 3 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain thr-48 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag certain certain sequence position certain certain certain sequence position certain hmh_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role prey prey cysteine-rich region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2A, 2C, 3D To examine whether these proteins interact in vivo, we coexpressed GFP-tagged Opy2ΔSR1 (Opy2ΔSR1-GFP) and HA-tagged Msb2Δ1 (Msb2Δ1-2×HA) in the yeast strain TM257 (ssk2Δ ssk22Δ), and coimmunoprecipitation experiments were conducted. msb2-opy2-2 1 3 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain thr-48 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Q06810:p.[Cys30Ala;Cys55Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A Wed May 02 17:51:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys30ala,cys55ala' Tue Jun 30 19:01:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys30Ala;Cys55Ala]' Q06810:p.[Cys33Ala;Cys58Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:56:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys33ala,cys58ala' Tue Jun 30 17:53:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys33Ala;Cys58Ala]' Q06810:p.[Cys30Ala;Cys42Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:45:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys30ala,cys42ala' Tue Jun 30 19:54:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys30Ala;Cys42Ala]' Q06810:p.[Cys33Ala;Cys39Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:41:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys33ala,cys39ala' Tue Jun 30 18:27:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys33Ala;Cys39Ala]' physical association physical association Fig. 5F, 6A The interaction was not substantially altered by osmostress msb2-opy2-4 1 3 unspecified role unspecified role bait bait P32334:p.Thr986Cys mutation mutation certain certain sequence position certain certain certain sequence position certain T C Wed May 02 15:31:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr986cys' Tue Jun 30 19:00:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr986Cys' c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain thr-48 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Q06810:p.Cys48Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:08:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys48ala' Tue Jun 30 18:48:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys48Ala' physical association physical association Fig. 6E, 7A The interaction was not substantially altered by osmostress