DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Foreign DNA capture during CRISPR-Cas adaptive immunity. Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature 2015 imex curation Nuñez JK., Harrington LB., Kranzusch PJ., Engelman AN., Doudna JA. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nuñez JK., Harrington LB., Kranzusch PJ., Engelman AN., Doudna JA. DIP original experiment: DIP-267203X Nature 2015 IMEx Foreign DNA capture during CRISPR-Cas adaptive immunity. Foreign DNA capture during CRISPR-Cas adaptive immunity. Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature 2015 imex curation Nuñez JK., Harrington LB., Kranzusch PJ., Engelman AN., Doudna JA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Nuñez JK., Harrington LB., Kranzusch PJ., Engelman AN., Doudna JA. DIP original experiment: DIP-267202X Nature 2015 IMEx Foreign DNA capture during CRISPR-Cas adaptive immunity. cas1_ecoli CRISPR-associated endonuclease Cas1 ygbT cas1 JW2725 b2755 protein protein ecoli Escherichia coli (strain K12) MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQAKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTWNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQPPAPPEDAQPVAIPLPVSLGDAGHRSS DIP protein Q46896 original sequence version: 87 DIP protein Q46896 original sequence version: 101 01A31BA98453D8A5 cas2_ecoli CRISPR-associated endoribonuclease Cas2 ygbF cas2 JW5438 b2754 protein protein ecoli Escherichia coli (strain K12) MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTPVDLDGLRLVSFLPV DIP protein P45956 original sequence version: 88 DIP protein P45956 original sequence version: 98 D1C159D924B477B4 ygbf-ygbt-6 2 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ygbf-ygbt-7 1 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction