DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268163X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268168X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions human Homo sapiens Human tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268169X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268166X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268164X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268167X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268165X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268170X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Science (New York, N.Y.) 2015 imex curation Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang L., Chen S., Ruan J., Wu J., Tong AB., Yin Q., Li Y., David L., Lu A., Wang WL., Marks C., Ouyang Q., Zhang X., Mao Y., Wu H. DIP original experiment: DIP-268162X Science (New York, N.Y.) 2015 IMEx Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. prgj_salty Protein PrgJ prgJ STM2872 protein protein salty Salmonella typhimurium MSIATIVPENAVIGQAVNIRSMETDIVSLDDRLLQAFSGSAIATAVDKQTITNRIEDPNLVTDPKELAISQEMISDYNLYVSMVSTLTRKGVGAVETLLRS 7AC04EF792D72598 flic_salty Flagellin fliC flaF hag Phase 1-I flagellin STM1959 protein protein salty Salmonella typhimurium MAQVINTNSLSLLTQNNLNKSQSALGTAIERLSSGLRINSAKDDAAGQAIANRFTANIKGLTQASRNANDGISIAQTTEGALNEINNNLQRVRELAVQSANSTNSQSDLDSIQAEITQRLNEIDRVSGQTQFNGVKVLAQDNTLTIQVGANDGETIDIDLKQINSQTLGLDTLNVQQKYKVSDTAATVTGYADTTIALDNSTFKASATGLGGTDQKIDGDLKFDDTTGKYYAKVTVTGGTGKDGYYEVSVDKTNGEVTLAGGATSPLTGGLPATATEDVKNVQVANADLTEAKAALTAAGVTGTASVVKMSYTDNNGKTIDGGLAVKVGDDYYSATQNKDGSISINTTKYTADDGTSKTALNKLGGADGKTEVVSIGGKTYAASKAEGHNFKAQPDLAEAAATTTENPLQKIDAALAQVDTLRSDLGAVQNRFNSAITNLGNTVNNLTSARSRIEDSDYATEVSNMSRAQILQQAGTSVLAQANQVPQNVLSLLR 4BD7849FA3B936BA 4BD7849FA3B936BA bir1e_mouse Baculoviral IAP repeat-containing protein 1e Naip5 Birc1e Naip-rs3 Neuronal apoptosis inhibitory protein 5 protein protein mouse Mus musculus Mouse MAEHGESSEDRISEIDYEFLPELSALLGVDAFQVAKSQEEEEHKERMKMKKGFNSQMRSEAKRLKTFETYDTFRSWTPQEMAAAGFYHTGVRLGVQCFCCSLILFGNSLRKLPIERHKKLRPECEFLQGKDVGNIGKYDIRVKRPEKMLRGGKARYHEEEARLESFEDWPFYAHGTSPRVLSAAGFVFTGKRDTVQCFSCGGSLGNWEEGDDPWKEHAKWFPKCEFLQSKKSSEEIAQYIQSYEGFVHVTGEHFVKSWVRRELPMVSAYCNDSVFANEELRMDMFKDWPQESPVGVEALVRAGFFYTGKKDIVRCFSCGGCLEKWAEGDDPMEDHIKFFPECVFLQTLKSSAEVIPTLQSQYALPEATETTRESNHGDAAAVHSTVVDLGRSEAQWFQEARSLSEQLRDNYTKATFRHMNLPEVCSSLGTDHLLSCDVSIISKHISQPVQEALTIPEVFSNLNSVMCVEGETGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPDQGLANIICAQLLGAGGCISEVCLSSSIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNRVRDIRLYLGTSLEIQEFPFYNTVSVLRKFFSHDIICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQNASAQDKFQDVTLFQSYMQYLSLKYKATAEPLQATVSSCGQLALTGLFSSCFEFNSDDLAEAGVDEDEKLTTLLMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQIDSPLKAINSFNIFLYYVSSHSSSKAAPTVVSHLLQLVDEKESLENMSENEDYMKLHPQTFLWFQFVRGLWLVSPESSSSFVSEHLLRLALIFAYESNTVAECSPFILQFLRGKTLALRVLNLQYFRDHPESLLLLRSLKVSINGNKMSSYVDYSFKTYFENLQPPAIDEEYTSAFEHISEWRRNFAQDEEIIKNYENIRPRALPDISEGYWKLSPKPCKIPKLEVQVNNTDAADQALLQVLMEVFSASQSIEFRLFNSSGFLESICPALELSKASVTKCSMSRLELSRAEQELLLTLPALQSLEVSETNQLPEQLFHNLHKFLGLKELCVRLDGKPNVLSVLPREFPNLLHMEKLSIQTSTESDLSKLVKFIQNFPNLHVFHLKCDFLSNCESLMAVLASCKKLREIEFSGRCFEAMTFVNILPNFVSLKILNLKDQQFPDKETSEKFAQALGSLRNLEELLVPTGDGIHQVAKLIVRQCLQLPCLRVLTFHDILDDDSVIEIARAATSGGFQKLENLDISMNHKITEEGYRNFFQALDNLPNLQELNICRNIPGRIQVQATTVKALGQCVSRLPSLIRLHMLSWLLDEEDMKVINDVKERHPQSKRLIIFWKLIVPFSPVILE DIP protein Q9R016 original sequence version: 82 DIP protein Q9R016 original sequence version: 113 DIP protein Q9R016 original sequence version: 118 DIP protein Q9R016 original sequence version: 115 04C04877908103EE nlrc4_mouse NLR family CARD domain-containing protein 4 Nlrc4 Card12 Ipaf Caspase recruitment domain-containing protein 12 Ice protease-activating factor protein protein mouse Mus musculus Mouse MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA DIP protein Q3UP24 original sequence version: 86 DIP protein Q3UP24 original sequence version: 90 DIP protein Q3UP24 original sequence version: 87 casp1_mouse Caspase-1 Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 Casp1 Il1bc protein protein mouse Mus musculus Mouse MADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKLANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEDSKGGHPSSSETKEEQNKEDGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIIIQACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFPGH DIP protein P29452 original sequence version: 87 DIP protein P29452 original sequence version: 158 DIP protein P29452 original sequence version: 123 DIP protein P29452 original sequence version: 122 3BEBCEFA67C69B03 bir1b_mouse Baculoviral IAP repeat-containing protein 1b Naip2 Birc1b Naip-rs6 Neuronal apoptosis inhibitory protein 2 protein protein mouse Mus musculus Mouse MAAQGEAVEEIICEFDDDLVSELSTLLRVDALSVLKRQQEEDHKTRMKMKKGFNSQMRSEAKRLKTFETYDKFRSWTPQEMAAAGFYHTGVKLGVQCFCCSLILFSTRLRKLPIENHKKLRPECEFLLGKDVGNIGKYDIRVKSPEKMLRGDKARYHEEEARLESFEDWPFYAHGTSPRVLSAAGFVFTGKRDTVQCFSCGGCLGNWEEGDDPWKEHAKWFPKCEFLQSKKSPEEITQYVQSYEGFLHVTGEHFVNSWVRRELPMVSAYCNDSVFANEELRMDTFKDWPHESPGAVEALVKAGLFYTGKRDIVQCFSCGGCMEKWAEGDNPIEDHTKFFPNCVFLQTLKSSAEVIPALQSHCALPEAMETTSESNHDDAAAVHSTVVDVSPSEAQELEPASSLVSVLCRDQDHSEAQGRGCASSGTYLPSTDLGQSEAQWLQEARSLSEQLRDTYTKATFRHMNLPEVYSSLGTDHLLSCDVSIISKHISQPVQGSLTIPEVFSNLNSVMCVEGEAGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPGQELAKIICAQLLGAGGCISEVCLSSIIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNKVRGIRPYLDTSLEIKEFPFYNTVSVLRKLFSHDIMRVRKFINYFGFHEELQGIHKTPLFVAAVCTDWFKNPSDQPFQDVALFKAYMQYLSLKHKGAAKPLQATVSSCGQLALTGLFSSCFEFNSDNLAEAGVDEDEELTTCLMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQINSPLKAMSIYHTFLKYVSSHPSSKAAPTVVSHLLQLVDEKESLENMSENEDYMKLHPEALLWIECLRGLWQLSPESFSLFISENLLRICLNFAHESNTVAACSPVILQFLRGRTLDLKVLSLQYFWDHPETLLLLKSIKISLNGNNWVQRIDFSLIEKSFEKVQPPTIDQDYAIAFQPINEVQKNLSEKKHIIKKYEDMKHQIPLNISTGYWKLSPKPYKIPKLEVQVTNTGPADQALLQVLMEVFSASQSIEFRLSDSSGFLESIRPALELSKASVTKCSMSRLELSREDQKLLLTLPTLQSLEVSETNQLPDQLFHNLHKFLGLKELCVRLDSKPDVLSVLPGEFPNLHHMEKLSIRTSTESDLSKLVKLIQNSPNLHVFHLKCNFLSNCEPLMTVLASCKKLREIEFSGRCFEAMTFVNILPNFVFLKILNLRDQQFPDKETSEKFAQALGSLRNLEKLFVPTGDGIHQVAKLIVRQCLQLPCLRVLVFAETLDDDSVLEIAKGATRGGFQKLENLDLTLNHKITEEGYRNFFQVLDNLPNLKNLDISRHIPECIQIQAITVKALGQCVSRLPSLTRLGMLSWLLDEEDIKVINDVKERHPQSKRLTVHWRWVVPFSPVIQK DIP protein Q9QUK4 original sequence version: 89 DIP protein Q9QUK4 original sequence version: 128 naip2-nlrc4-1 9 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 15 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 13 unspecified role unspecified role unspecified role unspecified role Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association naip5-nlrc4-2 6 12 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q3UP24:p.Arg285Asp R285D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R285D'. Wed May 02 15:04:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg285asp' Tue Jun 30 15:48:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg285Asp' Q3UP24:p.[Arg288Asp;His289Asp] R288D/H289D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H D certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R288D/H289D'. Wed May 02 18:06:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg288asp,his289asp' Tue Jun 30 18:31:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg288Asp;His289Asp]' human Homo sapiens Human physical association physical association nlrc4-1 5 13 unspecified role unspecified role unspecified role unspecified role Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction flic-naip5-2 3 12 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 11 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 13 unspecified role unspecified role unspecified role unspecified role Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association nlrc4-flic-1 4 12 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9R016:p.[Arg587Ala;Arg590Ala] R587A/R590A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R587A/R590A'. Wed May 02 17:27:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg587ala,arg590ala' Tue Jun 30 17:25:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg587Ala;Arg590Ala]' human Homo sapiens Human 11 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association nlrc4-flic-2 7 12 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 11 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q3UP24:p.[Glu122Ala;Asp123Ala;Asp125Ala] E122A/D123A/D125A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E122A/D123A/D125A'. Wed May 02 17:11:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu122ala,asp123ala,asp125ala' Tue Jun 30 19:35:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu122Ala;Asp123Ala;Asp125Ala]' human Homo sapiens Human association association naip2-nlrc4-2 1 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 15 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 13 unspecified role unspecified role unspecified role unspecified role Region 93-1024 Region 93-1024 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association nlrc4-4 8 13 unspecified role unspecified role unspecified role unspecified role Region 1-88 Region 1-88 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction casp1-nlrc4-1 2 14 unspecified role unspecified role unspecified role unspecified role Region 1-91 Region 1-91 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain TAMRA-label TAMRA-label tetramethyl rhodamine label tetramethyl rhodamine label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 1-88 Region 1-88 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction