IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. eLife 2015 imex curation Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. usually I would add competition expts with "zero" point - no competitor - but in these instances there is only a reaction/dissociation in presence of TAPBPR - or I have (probably) not done these correctly... "Peptide-receptive" HLA-A*02:01 and HLA-B*08:01 (HLA-B*08:01fos or HLA-B*08:01fos T134K molecules) were obtained by exposing the monomeric MHC class I complexes with UV labile peptides(for HLA-A*02:01= KILGFVFjV (j represents 3-amino-3-(2-nitro) phenyl-propionic acid and for HLA-B*08:01 =FLRGRAjGL) to ∼360 nm light for 20 min at 4°C ('UV exposed'). MHC class I molecules were subsequently allowed to bind to fluorescent peptides and dissociation of the fluorescent peptide was subsequently followed after the addition of excess non-labelled competitor peptide in the absence or presence of TAPBPR or other proteins. HLA-B*08:01fos or HLA-B*08:01fos T134K molecules HLA A*0201/HLA B:0801:-DNA encoding the "ER luminal domains" was used; but the residue numbering of these domains is not stated;it has been assumed to be the domains before the transmembrane region. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk Accepted 2017-MAY-03 AT 09:56 BST AT 09:56 BST by ORCHARD eLife 2015 imex curation I have replaced the Plant protein with Q6DPU2:- I have used this entry rather than D9J353, as it was an exact match to the sequence given in the Nature article, as in the Data-Proc comment I added - also a Reviewed entry - so presumably will not be "lost" TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. eLife 2015 imex curation Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line Human 721.221 721.221 HLA-A, B, C-null phenotype lymphoblastoid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. This cell line does not express endogenous HLA class 1 molecules. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. most IPs are using non-specific antibodies; i had hoped to get something out of Fig 7- but the HCA2 antibody targets HLA class 1 and there is no info as to which HLA molecules are expressed by KBM-7 cells - text says it expresses HLA-A2 - but not if it expresses HLA-B/C as well. lhb22@cam.ac.uk Accepted 2017-MAY-03 AT 09:56 BST AT 09:56 BST by ORCHARD eLife 2015 imex curation I have replaced the Plant protein with Q6DPU2:- I have used this entry rather than D9J353, as it was an exact match to the sequence given in the Nature article, as in the Data-Proc comment I added - also a Reviewed entry - so presumably will not be "lost" TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. eLife 2015 imex curation Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. "Peptide-receptive" HLA-A*02:01 and HLA-B*08:01 were obtained by exposing the monomeric MHC class I complexes with UV labile peptides(for HLA-A*02:01= KILGFVFjV (j represents 3-amino-3-(2-nitro) phenyl-propionic acid and for HLA-B*08:01 =FLRGRAjGL) to ∼360 nm light for 20 min at 4°C ('UV exposed'). The binding of fluorescent peptides to UV-exposed MHC class I was monitored in the absence and the presence of TAPBPR or I285K mutant. The Uniprot entry for the UV-labile peptide KILGFVFjV (j represents 3-amino-3-(2-nitro) phenyl-propionic acid) -UniProt Q6DPU2, influenza Matrix 1 epitope; this was derived from the sequence GILGFVFTL - see PMID:17406393 HLA A*0201/B0801:-DNA encoding the "ER luminal domains" was used; but the residue numbering of these domains is not stated;it has been assumed to be the domains before the transmembrane region. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk Accepted 2017-MAY-03 AT 09:56 BST AT 09:56 BST by ORCHARD eLife 2015 imex curation I have replaced the Plant protein with Q6DPU2:- I have used this entry rather than D9J353, as it was an exact match to the sequence given in the Nature article, as in the Data-Proc comment I added - also a Reviewed entry - so presumably will not be "lost" TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. eLife 2015 imex curation Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-721.220 human 721.220 lymphoblastoid cell line, tapasin, HLA-A/B negative, HLA-Cw*0102 positive Human 721.220 721.220 lymphoblastoid cell line, tapasin, HLA-A/B negative, HLA-Cw*0102 positive anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. not entered as an association as they do not usually transfect more than one molecule at a time lhb22@cam.ac.uk Accepted 2017-MAY-03 AT 09:56 BST AT 09:56 BST by ORCHARD eLife 2015 imex curation I have replaced the Plant protein with Q6DPU2:- I have used this entry rather than D9J353, as it was an exact match to the sequence given in the Nature article, as in the Data-Proc comment I added - also a Reviewed entry - so presumably will not be "lost" TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. eLife 2015 imex curation Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. lhb22@cam.ac.uk 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Hermann C., van Hateren A., Trautwein N., Neerincx A., Duriez PJ., Stevanović S., Trowsdale J., Deane JE., Elliott T., Boyle LH. Mapping is given in relation to UniProt full length molecules -including signal sequences- as signal peptide lengths given in this publication sometimes differ from those stated in Uniprot. supp Fig 2: I have NOT entered data without TPABPR or Tapasin for every figure, as the black line may only be random decay/dissociation or similar, rather than an interaction. HLA-A*02:01fos and beta2-microglobulin molecules made peptide receptive by UV exposure and were loaded with various labelled peptides and the samples split. Excess unlabelled FLPSDCFPSV with or without the proteins TAPBPR or Tapasin-Jun were then added. lhb22@cam.ac.uk Accepted 2017-MAY-03 AT 09:56 BST AT 09:56 BST by ORCHARD eLife 2015 imex curation I have replaced the Plant protein with Q6DPU2:- I have used this entry rather than D9J353, as it was an exact match to the sequence given in the Nature article, as in the Data-Proc comment I added - also a Reviewed entry - so presumably will not be "lost" b2mg_human Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM DIP protein P61769 original sequence version: null DIP protein P61769 original sequence version: 138 DIP protein P61769 original sequence version: 134 DIP protein P61769 original sequence version: 135 DIP protein P61769 original sequence version: 116 DIP protein P61769 original sequence version: 60 DIP protein P61769 original sequence version: 79 DIP protein P61769 original sequence version: 87 DIP protein P61769 original sequence version: 93 DIP protein P61769 original sequence version: 94 DIP protein P61769 original sequence version: 91 DIP protein P61769 original sequence version: 106 DIP protein P61769 original sequence version: 111 AFD2DBEF07DCEF27 hs90a_human Heat shock protein HSP 90-alpha HSP90AA1 HSP90A HSPC1 Heat shock 86 kDa Renal carcinoma antigen NY-REN-38 HSPCA Lipopolysaccharide-associated protein 2 protein protein human Homo sapiens Human MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLIPNKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVTVITKHNDDEQYAWESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKHSQFIGYPITLFVEKERDKEVSDDEAEEKEDKEEEKEKEEKESEDKPEIEDVGSDEEEEKKDGDKKKKKKIKEKYIDQEELNKTKPIWTRNPDDITNEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENRKKKNNIKLYVRRVFIMDNCEELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNLVKKCLELFTELAEDKENYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQKHIYYITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD DIP protein P07900 original sequence version: 197 DIP protein P07900 original sequence version: 116 DIP protein P07900 original sequence version: 150 DIP protein P07900-1 original sequence version: 158 DIP protein P07900 original sequence version: 207 DIP protein P07900 original sequence version: 192 DIP protein P07900 original sequence version: 156 969F65FCC0BC86FD mov10_human Helicase MOV-10 Moloney leukemia virus 10 protein MOV10 KIAA1631 Armitage homolog protein protein human Homo sapiens Human MPSKFSCRQLREAGQCFESFLVVRGLDMETDRERLRTIYNRDFKISFGTPAPGFSSMLYGMKIANLAYVTKTRVRFFRLDRWADVRFPEKRRMKLGSDISKHHKSLLAKIFYDRAEYLHGKHGVDVEVQGPHEARDGQLLIRLDLNRKEVLTLRLRNGGTQSVTLTHLFPLCRTPQFAFYNEDQELPCPLGPGECYELHVHCKTSFVGYFPATVLWELLGPGESGSEGAGTFYIARFLAAVAHSPLAAQLKPMTPFKRTRITGNPVVTNRIEEGERPDRAKGYDLELSMALGTYYPPPRLRQLLPMLLQGTSIFTAPKEIAEIKAQLETALKWRNYEVKLRLLLHLEELQMEHDIRHYDLESVPMTWDPVDQNPRLLTLEVPGVTESRPSVLRGDHLFALLSSETHQEDPITYKGFVHKVELDRVKLSFSMSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWLLWPMLFPVAPRDVPLLPSDVKLKLYDRSLESNPEQLQAMRHIVTGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLPKAHILACAPSNSGADLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKPCCNWDAKKGEYVFPAKKKLQEYRVLITTLITAGRLVSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMEVKETGDPGGQLVLAGDPRQLGPVLRSPLTQKHGLGYSLLERLLTYNSLYKKGPDGYDPQFITKLLRNYRSHPTILDIPNQLYYEGELQACADVVDRERFCRWAGLPRQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKLLLAPSSKKGKARLSPRSVGVISPYRKQVEKIRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFNVAVTRAKALLIIVGNPLLLGHDPDWKVFLEFCKENGGYTGCPFPAKLDLQQGQNLLQGLSKLSPSTSGPHSHDYLPQEREGEGGLSLQVEPEWRNEL DIP protein Q9HCE1 original sequence version: 55 DIP protein Q9HCE1 original sequence version: 74 28A8B3BF9B5B54FC hlaf_human HLA class I histocompatibility antigen, alpha chain F HLA-F HLA-5.4 HLAF CDA12 HLA F antigen Leukocyte antigen F MHC class I antigen F protein protein human Homo sapiens Human MAPRSLLLLLSGALALTDTWAGSHSLRYFSTAVSRPGRGEPRYIAVEYVDDTQFLRFDSDAAIPRMEPREPWVEQEGPQYWEWTTGYAKANAQTDRVALRNLLRRYNQSEAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADTVAQITQRFYEAEEYAEEFRTYLEGECLELLRRYLENGKETLQRADPPKAHVAHHPISDHEATLRCWALGFYPAEITLTWQRDGEEQTQDTELVETRPAGDGTFQKWAAVVVPPGEEQRYTCHVQHEGLPQPLILRWEQSPQPTIPIVGIVAGLVVLGAVVTGAVVAAVMWRKKSSDRNRGSYSQAAV D4782968A67E9B7D 1b08_human_deprecated MHC class I antigen B*8 HLA-B HLAB protein protein human Homo sapiens Human MLVMAPRTVLLLLSAALALTETWAGSHSMRYFDTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQIFKTNTQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHNQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAARVAEQDRAYLEGTCVEWLRRYLENGKDTLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA This protein is not up-to-date anymore with the uniprot protein because of feature conflicts. 9B2A9760021680DF capsd_hbvh3 Capsid protein C Core antigen Core protein HBcAg p21.5 protein protein isolate Nicaragua/2928Nic/1997 Hepatitis B virus genotype H subtype adw4 HBV-H MDIDPYKEFGASVELLSFLPSDFFPSVRDLLDTASALYRDALESPEHCTPNHTALRQAILCWGELMTLASWVGNNLEDPAARDLVVNYVNTNMGLKIRQLLWFHISCLTFGRDTVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRQRGRAPRRRTPSPRRRRSQSPRRRRSQSPASQC 271C191A0B79B432 1a68_human HLA class I histocompatibility antigen, A-68 alpha chain HLA-A HLAA Aw-68 HLA class I histocompatibility antigen, A-28 alpha chain MHC class I antigen A*68 protein protein human Homo sapiens Human MAVMAPRTLVLLLSGALALTQTWAGSHSMRYFYTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDRNTRNVKAQSQTDRVDLGTLRGYYNQSEAGSHTIQMMYGCDVGSDGRFLRGYRQDAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQWRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWVAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEPSSQPTIPIVGIIAGLVLFGAVITGAVVAAVMWRRKSSDRKGGSYSQAASSDSAQGSDVSLTACKV 78539C59DB8B1DFC ebna3_ebvg Epstein-Barr nuclear antigen 3 Epstein-Barr nuclear antigen 3A BLRF3-BERF1 EBNA3 protein protein ebvg Epstein-Barr virus (strain B95-8) Human herpesvirus 4 MDKDRPGDPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELSDSSSEKEAEDAQLEPAQKGTKRKRVDHDAGGSAPARPMLPPQPDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQISNTEMYIMYAMAIRQAIRDRRRNPASRRDQAKWRLQTLAAGWPMGYQAYSSWMYSYTDHQTAPTFVQLQATLGCTGGRRCHVTFSAGTFKPPRCTPGDRQWLYVQSSVGNIVQSCNPRYSIFFDYMAIHRSLTKIWEEVLTPDQRVSFMEFLGFLQRTDLSYIKSFVSDALGTTSIQTPWIDDNSSTETAQAWNAGFLRGRAYGLDLLRTEGEHVEGATGETREESEDTESDGDDEDLPCIVSRGGPKVKRPPIFIRRLHRLLLMRAGKRTEQGKEVLEKARGSTYGTPRPPVPKPRPEVPQSDETATSHGSAQVPEPPTIHLAAQGMAYPLHEQRGMAPCPVAQAPHTPLPPVSPGDQLPGVSSDGRVACAPVPAPAGPIVRPWEPSLTQAAGQAFAPVRPQHMPVEPVPVPTVALERPVYPKPVRPAPPKIAMQGPGETSGIRRARERWRPAPWTPNPPRSPSQMSVRDRLARLRAEAQVKQASVEVQPPQVTQVSPQQPMEGPGAPFSQVADVVHTPGVPAMQPQYFDLPLIQPISQGAPVAPLRASMGPVPPVPATQPQYFDIPLTEPINQGASAAHFLPQQPMEGPLVPEQWMFPGAALSQSVRPGVAQSQYFDLPLTQPITHGAPAAHFLHQPPMEGPWVPEQWMFQGAPPSQGTDVVQHQLDALGYPLHALNHPGVPVSPAVNQYHLSQAAFGLPIDEDESGEGSDTSEPCEALDLSIHGRPCPQAPEWPVQGEGGQDATEVLDLSIHGRPRPRTPEWPVQGESGQNVTDHEPRRVVVSAIVHMCQDDEFPDLQDPPDEA DABB76CCC45C2E5C etfb_human Electron transfer flavoprotein subunit beta ETFB FP585 protein protein human Homo sapiens Human MAELRVLVAVKRVIDYAVKIRVKPDRTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEVIAVSCGPAQCQETIRTALAMGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDLVLLGKQAIDDDCNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI 47E6EAEF50EB2C80 m1_i02a1 Matrix protein 1 M protein protein Influenza A virus (strain A/Guinea fowl/Hong Kong/38/2002 H5N1 genotype X0) Influenza A virus (strain A/Guinea fowl/Hong Kong/38/2002 H5N1 genotype X0) ETYVLSIVPSGPLKAEIAQRLEDVFAGKNTDLEALMEWLKTRPILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLYKKLKREITFHGAKEVALSYSTGALASCMGLIYNRMGTVTTEVAFGLVCATCEQIADSQHRSHRQMATITNPLIRHENRMVLASTTAKAMEQMAGSSEQAAEAMEVASQARQMVQAMRTIGTHPNSSTGLRDNLLENLQAYQNRMGVQMQRFK F10A531595088BCF gag_hv1w2 Gag polyprotein gag Pr55Gag protein protein isolate WMJ22 Human immunodeficiency virus type 1 group M subtype B HIV-1 MGARASVLSGGELDKWEKIRLRPGGKKKYRLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEKKDTKEALDKIEEEQNKCKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPTTIMMQKGNFRNQRKT F965F184AD63ECCD pp65_hcmvt 65 kDa phosphoprotein UL83 64 kDa matrix phosphoprotein GP64 Phosphoprotein UL83 Tegument protein UL83 protein protein hcmvt Human cytomegalovirus (strain Towne) Human herpesvirus 5 MASVLGPISGHVLKAVFSRGDTPVLPHETRLLQTGIHVRVSQPSLILVSQYTPDSTPCHRGDNQLQVQHTYFTGSEVENVSVNVHNPTGRSICPSQEPMSIYVYALPLKMLNIPSINVHHYPSAAERKHRHLPVADAVIHASGKQMWQARLTVSGLAWTRQQNQWKEPDVYYTSAFVFPTKDVALRHVVCAHELVCSMENTRATKMQVIGDQYVKVYLESFCEDVPSGKLFMHVTLGSDVEEDLTMTRNPQPFMRPHERNGFTVLCPKNMIIKPGKISHIMLDVAFTSHEHFGLLCPKSIPGLSISGNLLMNGQQIFLEVQAIRETVELRQYDPVAALFFFDIDLLLQRGPQYSEHPTFTSQYRIQGKLEYRHTWDRHDEGAAQGDDDVWTSGSDSDEELVTTERKTPRVTGGGAMAGASTSAGRKRKSASSATACTAGVMTRGRLKAESTVAPEEDTDEDSDNEIHNPAVFTWPPWQAGILARNLVPMVATVQGQNLKYQEFFWDANDIYRIFAELEGVWQPAAQPKRRRHRQDALPGPCIASTPKKHRG C56D11AEB01F3C23 hlab_human HLA class I histocompatibility antigen, B alpha chain Human leukocyte antigen B HLA-B HLAB protein protein human Homo sapiens Human MLVMAPRTVLLLLSAALALTETWAGSHSMRYFYTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQIYKAQAQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDKLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA 5E5A7BDE031403D6 smc2_human Structural maintenance of chromosomes protein 2 PRO0324 SMC2 CAPE SMC2L1 Chromosome-associated protein E XCAP-E homolog protein protein human Homo sapiens Human MHIKSIILEGFKSYAQRTEVNGFDPLFNAITGLNGSGKSNILDSICFLLGISNLSQVRASNLQDLVYKNGQAGITKASVSITFDNSDKKQSPLGFEVHDEITVTRQVVIGGRNKYLINGVNANNTRVQDLFCSVGLNVNNPHFLIMQGRITKVLNMKPPEILSMIEEAAGTRMYEYKKIAAQKTIEKKEAKLKEIKTILEEEITPTIQKLKEERSSYLEYQKVMREIEHLSRLYIAYQFLLAEDTKVRSAEELKEMQDKVIKLQEELSENDKKIKALNHEIEELEKRKDKETGGILRSLEDALAEAQRVNTKSQSAFDLKKKNLACEESKRKELEKNMVEDSKTLAAKEKEVKKITDGLHALQEASNKDAEALAAAQQHFNAVSAGLSSNEDGAEATLAGQMMACKNDISKAQTEAKQAQMKLKHAQQELKNKQAEVKKMDSGYRKDQEALEAVKRLKEKLEAEMKKLNYEENKEESLLEKRRQLSRDIGRLKETYEALLARFPNLRFAYKDPEKNWNRNCVKGLVASLISVKDTSATTALELVAGERLYNVVVDTEVTGKKLLERGELKRRYTIIPLNKISARCIAPETLRVAQNLVGPDNVHVALSLVEYKPELQKAMEFVFGTTFVCDNMDNAKKVAFDKRIMTRTVTLGGDVFDPHGTLSGGARSQAASILTKFQELKDVQDELRIKENELRALEEELAGLKNTAEKYRQLKQQWEMKTEEADLLQTKLQQSSYHKQQEELDALKKTIEESEETLKNTKEIQRKAEEKYEVLENKMKNAEAERERELKDAQKKLDCAKTKADASSKKMKEKQQEVEAITLELEELKREHTSYKQQLEAVNEAIKSYESQIEVMAAEVAKNKESVNKAQEEVTKQKEVITAQDTVIKAKYAEVAKHKEQNNDSQLKIKELDHNISKHKREAEDGAAKVSKMLKDYDWINAERHLFGQPNSAYDFKTNNPKEAGQRLQKLQEMKEKLGRNVNMRAMNVLTEAEERYNDLMKKKRIVENDKSKILTTIEDLDQKKNQALNIAWQKVNKDFGSIFSTLLPGANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGGQRSLVALSLILSMLLFKPAPIYILDEVDAALDLSHTQNIGQMLRTHFTHSQFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQCQNGKISKEAKSKAKPPKGAHVEV DIP protein O95347 original sequence version: 87 DIP protein O95347 original sequence version: 95 DIP protein O95347 original sequence version: 139 15EBB56B31FC1364 tpsn_human Tapasin TAP-binding protein TAP-associated protein NGS-17 TAPBP NGS17 TAPA protein protein human Homo sapiens Human MKSLSLLLAVALGLATAVSAGPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPRVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSVGLFLSAFLLLGLFKALGWAAVYLSTCKDSKKKAE 7340549519B288AD 1a02_human HLA class I histocompatibility antigen, A-2 alpha chain HLA-A HLAA Human leukocyte antigen A*02 protein protein human Homo sapiens Human MAVMAPRTLVLLLSGALALTQTWAGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEPSSQPTIPIVGIIAGLVLFGAVITGAVVAAVMWRRKSSDRKGGSYSQAASSDSAQGSDVSLTACKV Deprecated HLA-A AC, merged into a different UniProt canonical but we have mutation features in variable positions. Allele =A*02:01:01:01 Allele =A*02:01:01:01 according to IPD-IMGT/HLA database B54A97B24B337C08 tpsnr_human Tapasin-related protein TAPBPL TAP-binding protein-like TAP-binding protein-related protein Tapasin-like protein protein human Homo sapiens Human MGTQEGWCLLLCLALSGAAETKPHPAEGQWRAVDVVLDCFLAKDGAHRGALASSEDRARASLVLKQVPVLDDGSLEDFTDFQGGTLAQDDPPIIFEASVDLVQIPQAEALLHADCSGKEVTCEISRYFLQMTETTVKTAAWFMANMQVSGGGPSISLVMKTPRVTKNEALWHPTLNLPLSPQGTVRTAVEFQVMTQTQSLSFLLGSSASLDCGFSMAPGLDLISVEWRLQHKGRGQLVYSWTAGQGQAVRKGATLEPAQLGMARDASLTLPGLTIQDEGTYICQITTSLYRAQQIIQLNIQASPKVRLSLANEALLPTLICDIAGYYPLDVVVTWTREELGGSPAQVSGASFSSLRQSVAGTYSISSSLTAEPGSAGATYTCQVTHISLEEPLGASTQVVPPERRTALGVIFASSLFLLALMFLGLQRRQAPTGLGLLQAERWETTSCADTQSSHLHEDRTARVSQPS FA39C335FC22960E ms18a_human Protein Mis18-alpha MIS18A FAPP1-associated protein 1 C21orf46 FASP1 C21orf45 protein protein human Homo sapiens Human MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIESYVLGSSEKQIVSEDKELFNLESRVEIEKSLTQMEDVLKALQMKLWEAESKLSFATCKS A07522806C4B6221 tapbpl-hla_a-3 3 11 competitor competitor competitor neutral component neutral component tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 14:59:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 20:25:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role neutral component neutral component Q6DPU2:p.[Gly51Lys;Thr58_Leu59delinsXaaVal] mutation mutation certain certain sequence position certain certain certain sequence position certain G K certain certain sequence position certain certain certain sequence position certain TL XV Tue May 09 10:24:07 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'thr58xaa,gly51lys,leu59val' Wed May 02 16:40:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly51lys,thr_leu58-59xaa_val' Tue Jun 30 16:11:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly51Lys;Thr58_Leu59delinsXaaVal]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BX59:p.Ile285Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I K Wed May 02 14:43:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile285lys' Tue Jun 30 17:32:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile285Lys' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 33 30 35 physical association physical association 2 C HLA-A*0201 allele b2m-c-1 1 17 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 17:35:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 16:50:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component Q9BX59:p.Ile285Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I K Wed May 02 15:58:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile285lys' Tue Jun 30 18:35:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile285Lys' luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 52 49 46 physical association physical association 2 B HLA-A*0201 allele In the absence of TAPBPR there is no dissociation. b2m-c-2 1 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:21:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:53:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 15:57:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:38:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 70 67 64 physical association physical association 2 A HLA-A*0201 allele In the absence of TAPBPR there is no dissociation. b2m-c-3 3 15 unspecified role unspecified role neutral component neutral component Q6DPU2:p.[Gly51Lys;Thr58_Leu59delinsXaaVal] mutation mutation certain certain sequence position certain certain certain sequence position certain G K certain certain sequence position certain certain certain sequence position certain TL XV Tue May 09 10:32:14 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'thr58xaa,gly51lys,leu59val' Wed May 02 18:03:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly51lys,thr_leu58-59xaa_val' Tue Jun 30 17:48:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly51Lys;Thr58_Leu59delinsXaaVal]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 20:03:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:35:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component Q9BX59:p.Ile285Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I K Wed May 02 18:20:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile285lys' Tue Jun 30 19:21:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile285Lys' luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 81 87 79 physical association physical association 2 D HLA-A*0201 allele Mixtures of the high affinity labelled peptide FLPSDC*FPSV and a variable concentration of the peptide NLVPMVATV were added. In the presence of TAPBPR, the lower affinity peptide NLVPMVATV became a poorer competitor. I cannot add the low affinity peptide as an interactor as it would be a competitor to a competitor - no known role! b2m-c-4 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 15:57:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:01:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:17:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:40:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 99 105 102 physical association physical association 2 -supplement 1A HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-5 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:17:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 18:41:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 121 118 116 physical association physical association 2 -supplement 1B, figure 2 E, G HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone The half-life of peptides in the presence of TAPBPR does not follow the same trend as for the presence of Tapasin;TAPBPR enhanced dissociation of KLVK*EVIAV and FLPSDC*FPSV to a much smaller extent than was apparent in the presence of tapasin-jun or absence of either cofactor (competitor only). I have given up with these; not sure what to enter - especially for the YLEN....far RHS peptide, whose result=0%- does this mean dissociation was very very quick, or did not happen at all? so just entered a comment. b2m-c-6 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 16:36:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 19:07:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 19 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 137 134 132 physical association physical association 2 -supplement 1C HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-7 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 17:17:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 16:57:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 153 148 150 physical association physical association 2 -supplement 1D HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-8 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 16:39:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 19:27:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 166 164 169 physical association physical association 2 -supplement 1E HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-9 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:10:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 20:19:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 180 185 182 physical association physical association 2 -supplement 1F HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-10 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 17:20:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:52:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 196 201 198 physical association physical association 2 -supplement 1G HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-11 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 14:57:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 19:25:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 19:49:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 18:42:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 218 212 215 physical association physical association 2 -supplement 1Ajun HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-13 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:59:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:51:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 234 229 231 physical association physical association 2 -supplement 1Bjun, figure 2 E/F HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone Figure 2 E/F: the half-life hierarchy for tapasin was similar to the hierarchy observed in the absence of either cofactor (competitor only), with the exception of the dissociation of GLDDIKDLK*V whose dissociation was relatively much slower. I have given up with these; not sure what to enter - especially for the YLEN....far RHS peptide, whose result=0%- does this mean dissociation was very very quick, or did not happen at all? so just entered a comment. b2m-c-14 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:35:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 18:15:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 19 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 247 250 245 physical association physical association 2 -supplement 1Cjun HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-15 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 15:29:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:45:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 263 266 261 physical association physical association 2 -supplement 1Djun HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-16 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 19:49:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:30:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 277 282 279 physical association physical association 2 -supplement 1Ejun HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-17 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 17:28:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 17:09:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 293 295 298 physical association physical association 2 -supplement 1Fjun HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone b2m-c-18 5 11 competitor competitor competitor neutral component neutral component P0C6I6:p.Phe23Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 14:58:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe23cys' Tue Jun 30 19:33:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe23Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range jun_leucine_zipper_tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 311 309 314 physical association physical association 2 -supplement 1Gjun HLA-A*0201 allele The dissociation rates of all peptides were enhanced in the presence of TAPBPR and in the presence of tapasin-jun as compared to competing peptide alone tapbpl-hla_a-5 2 21 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 22 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 3-1, 8A tapbpl-hla_a-6 2 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 22 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 3-2 tapbpl-hla_b-1 2 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 22 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 3-3 weak reaction tapbpl-hla_b-2 2 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 22 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 3-4 b2m-c-12 1 16 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 16 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p.Thr158Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T K Wed May 02 16:27:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr158lys' ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 338 336 341 physical association physical association 4 A/B HLA-B*0801 allele TAPBPR did not significantly enhance dissociation of two additional peptides, ELRSRK*WAI and FLRGRK*YGL from HLA-B*08:01. (Figs 4 C/D) b2m-gag-1 3 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q3KST2:p.Tyr331Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Y X Wed May 02 20:20:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr331xaa' Tue Jun 30 17:37:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr331Xaa' chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component mature_protein sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 16 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tamra_tag rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminal_leucine_zipper tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p.Thr158Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T K Wed May 02 16:16:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr158lys' ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role neutral component neutral component luminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 358 353 350 physical association physical association 4 E HLA-B*0801 allele The enhancement of binding by TAPBPR was only observed when using the EIYK*RWIIL peptide. 213423q4-1 4 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 22 unspecified role unspecified role bait bait physical association physical association 8A tapbpl-hla_b-3 4 21 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 22 unspecified role unspecified role bait bait physical association physical association 8Ai