IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ T cell receptor reversed polarity recognition of a self-antigen major histocompatibility complex. T cell receptor reversed polarity recognition of a self-antigen major histocompatibility complex. Nature immunology 2015 imex curation Beringer DX., Kleijwegt FS., Wiede F., van der Slik AR., Loh KL., Petersen J., Dudek NL., Duinkerken G., Laban S., Joosten A., Vivian JP., Chen Z., Uldrich AP., Godfrey DI., McCluskey J., Price DA., Radford KJ., Purcell AW., Nikolic T., Reid HH., Tiganis T., Roep BO., Rossjohn J. hugh.reid@monash.edu, tony.tiganis@monash.edu,B.O.Roep@lumc.nl,jamie.rossjohn@monash.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Beringer DX., Kleijwegt FS., Wiede F., van der Slik AR., Loh KL., Petersen J., Dudek NL., Duinkerken G., Laban S., Joosten A., Vivian JP., Chen Z., Uldrich AP., Godfrey DI., McCluskey J., Price DA., Radford KJ., Purcell AW., Nikolic T., Reid HH., Tiganis T., Roep BO., Rossjohn J. TCR/MHC-class2/proinsulin_peptide complex. The TCR was derived from two clones of in vitro–induced Treg cells. hugh.reid@monash.edu, tony.tiganis@monash.edu,B.O.Roep@lumc.nl,jamie.rossjohn@monash.edu Accepted 2017-MAY-15 AT 08:57 BST AT 08:57 BST by ORCHARD Nature immunology 2015 imex curation clone_fs18_tcr_alpha_chain protein protein human Homo sapiens Human MQQVKQNSPSLSVQEGRISILNCDYTNSMFDYFLWYKKYPAEGPTFLISISSIKDKNEDGRFTVFLNKSAKHLSLHIVPSQPGDSAVYFCAASVYAGGTSYGKLTFGQGTILTVHPNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS clone_fs17_tcr_alpha_chain protein protein human Homo sapiens Human MQQVKQNSPSLSVQEGRISILNCDYTNSMFDYFLWYKKYPAEGPTFLISISSIKDKNEDGRFTVFLNKSAKHLSLHIVPSQPGDSAVYFCAASSSAGGTSYGKLTFGQGTILTVHPNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS a0a1v1igj9_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKFPGGSCMAALTVTLMVLSSPLALAGDTRPRFLEQVKHECHFFNGTERVRFLDRYFYHQEEYVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQKRAAVDTYCRHNYGVGESFTVQRRVYPEVTVYPAKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKTGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSLTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS 8116E91DA38294E5 clone_fs18_tcr_beta_chain protein protein human Homo sapiens Human MNAGVTQTPKFRVLKTGQSMTLLCAQDMNHEYMYWYRQDPGMGLRLIHYSVGEGTTAKGEVPDGYNVSRLKKQNFLLGLESAAPSQTSVYFCASRPRRDNEQFFGPGTRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFFPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD dra_human HLA class II histocompatibility antigen, DR alpha chain HLA-DRA MHC class II antigen DRA HLA-DRA1 protein protein human Homo sapiens Human MAISGVPVLGFFIIAVLMSAQESWAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGLRKSNAAERRGPL 3CD1CDBA89D92350 clone_fs17_tcr_beta_chain protein protein human Homo sapiens Human MNAGVTQTPKFRVLKTGQSMTLLCAQDMNHEYMYWYRQDPGMGLRLIHYSVGEGTTAKGEVPDGYNVSRLKKQNFLLGLESAAPSQTSVYFCASRPRDPVTQYFGPGTRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFFPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD ins_human Insulin INS protein protein human Homo sapiens Human MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN DIP protein P01308 original sequence version: 184 DIP protein P01308 original sequence version: 104 DIP protein P01308 original sequence version: 177 Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver mint homomint Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730] C2C3B23B85E520E5 clone_fs18_tcr_alpha_chain-clone_fs18_tcr_beta_chain-2 1 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293s Human 293S cell line Human 293s 293S cell line 6 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293s Human 293S cell line Human 293s 293S cell line 2 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association Table 1 P01903:- there is a C terminal expression tag TSGDDDDK:- the DDK may be FLAG - but no mention of this in methods section, and from info provided, I dont see how this would be possible, so not entered. Similarly for P13760: c terminal TGGDDDDK 2.5A RFree =0.196 RWork =0.16 clone_fs18_tcr_alpha_chain-clone_fs18_tcr_beta_chain-1 1 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293s Human 293S cell line Human 293s 293S cell line 6 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293s Human 293S cell line Human 293s 293S cell line 3 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association Table 1-2 P01903:- there is NO C terminal expression tag TSGDDDDK shown here!: P13760: c terminal TGGDDDDK as for other PDBe entry.....? (not entered) 4A RFree =0.283 RWork =0.224