DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structure (London, England : 1993) 2015 imex curation Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. DIP original experiment: DIP-267311X Structure (London, England : 1993) 2015 IMEx Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structure (London, England : 1993) 2015 imex curation Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. DIP original experiment: DIP-267309X Structure (London, England : 1993) 2015 IMEx Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structure (London, England : 1993) 2015 imex curation Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. DIP original experiment: DIP-267308X Structure (London, England : 1993) 2015 IMEx Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structure (London, England : 1993) 2015 imex curation Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yin J., Schoeffler AJ., Wickliffe K., Newton K., Starovasnik MA., Dueber EC., Harris SF. DIP original experiment: DIP-267310X Structure (London, England : 1993) 2015 IMEx Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. ubp1_human Ubiquitin carboxyl-terminal hydrolase 1 USP1 Ubiquitin-specific-processing protease 1 Deubiquitinating enzyme 1 Ubiquitin thioesterase 1 protein protein human Homo sapiens Human MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL DIP protein O94782 original sequence version: 141 50AA2817A60810AF id2_human DNA-binding protein inhibitor ID-2 Inhibitor of DNA binding 2 ID2 BHLHB26 Class B basic helix-loop-helix protein 26 Inhibitor of differentiation 2 protein protein human Homo sapiens Human MKAFSPVRSVRKNSLSDHSLGISRSKTPVDDPMSLLYNMNDCYSKLKELVPSIPQNKKVSKMEILQHVIDYILDLQIALDSHPTIVSLHHQRPGQNQASRTPLTTLNTDISILSLQASEFPSELMSNDSKALCG DIP protein Q02363 original sequence version: 133 DIP protein Q02363 original sequence version: 90 DIP protein Q02363 original sequence version: 144 A0D98B96396EB11E p62068-1 Ubiquitin carboxyl-terminal hydrolase 46 USP46 Deubiquitinating enzyme 46 Ubiquitin thioesterase 46 Ubiquitin-specific-processing protease 46 protein protein human Homo sapiens Human MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE DIP protein P62068-1 original sequence version: 99 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q8taf3-1 WD repeat-containing protein 48 WDR48 KIAA1449 UAF1 USP1-associated factor 1 WD repeat endosomal protein p80 protein protein human Homo sapiens Human MAAHHRQNTAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQDPYIASMEHHTDWVNDIVLCCNGKTLISASSDTTVKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQIFLWDVNTLTALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTDNVKALLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTDLRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLCTQPDQVIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWFSVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDEEENEVNHVNGEQENRVQKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMPKFNKIPFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPGEQEKEEDIAVLAEEKIELLCQDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST DIP protein Q8TAF3-1 original sequence version: 129 usp1-wdr48-1 4 9 unspecified role unspecified role prey prey Q8TAF3-1:p.[Lys214Glu;Trp256Ala;Arg272Asp] K214E/W256A/R272D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R D certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K214E/W256A/R272D'. Wed May 02 16:02:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys214glu,trp256ala,arg272asp' Tue Jun 30 16:13:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys214Glu;Trp256Ala;Arg272Asp]' HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 5 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association usp46-wdr48-1 3 7 unspecified role unspecified role bait bait trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role prey prey Q8TAF3-1:p.Ser170Tyr S170Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S Y This feature has been corrected as a result of our quality control procedures. The original label was 'S170Y'. Wed May 02 19:06:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser170tyr' Tue Jun 30 19:52:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser170Tyr' Q8TAF3-1:p.[Lys214Glu;Trp256Ala;Arg272Asp] K214E/W256A/R272D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R D certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K214E/W256A/R272D'. Wed May 02 19:50:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys214glu,trp256ala,arg272asp' Tue Jun 30 18:21:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys214Glu;Trp256Ala;Arg272Asp]' trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction usp1-wdr48-2 2 9 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait O94782:p.Glu444Lys E444K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E444K'. Wed May 02 15:56:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu444lys' Tue Jun 30 19:24:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu444Lys' Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association id2-ub-1 1 8 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human 6 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association