DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266296X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266304X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266299X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266300X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266301X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266302X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266297X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266303X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science (New York, N.Y.) 2015 imex curation Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266298X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. g0s6m7_chatd Probable vacuolar protein sorting-associated protein 16 homolog CTHT_0026760 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MDTAHPTASWEQLGERFYRKIQLYTQVFDQDFDLDNYIVTGAPYGGAIALYRDDEKLVAYQPSRSSRPTIDICSLSGKLLRRISWDQGPIKGVGWSEDEKLLIVMVDGTVRCYFDLQSEFTQFSLGHGAEEHGVKSCRFYSHGLVALLGNNALVSVSSYDEPRPKLLASPPEGRVYSWNIIPPAYSLSRSVEVLLSVNQTIYVCDASECEDRFLDIGPFSHIAVSPNGRFCALYTTTGKVHVITSDFQSRLSEHDTKSKIAPNYFEWCGNDAVVIAWDDEVHLVGPSGSLARFFYDSGRIHLIPDFDGVRILANDRCDFLQKVPDVIEEVFGLGADSPASILLDAVEQLEMKSPKADDNIQLIRPHLVEAVDTCVSAAGQEFSIHWQKQLLKAASFGKSVLDIYNSDDFVDMCETLRVLNAVRFYEVGLPLSYEQYQRLSPSGLISRLLNRHEYLLAIRIADHLRLPTDKIHVHWASAKVRLGSEDDDTICRKIVEKLSGKPGISFEVIARTAYEEGRTRLATELLNHEPRAGRQVPLLLSMEEDELALDKAIESGDTDLIYFVIHQLRRKLPLASFFRVVSSRPTASAMVEALARNSDGDGNEDTALLKDLYYQDDRRLDGASVFIREALQQPETRTASDKLDLAANLLQGNQKEHVFELGALKEAKMLLLNQTMFKLIKLGYHGRAKKIQSEFKVPERVAWWIRLQALVAKRDWNEIEEISRQRKSPIGWEPFFNQVLQAGNPRLAATFIPKCTNLEPGQTITMYEKCGMRVKAAEEAVRLKDTEAWNRLLEAAGRNTAEGREIERLGATVFKK DIP protein G0S6M7 original sequence version: 13 DIP protein G0S6M7 original sequence version: 18 g0s5g3_chatd V-SNARE coiled-coil homology domain-containing protein CTHT_0032860 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MASSSTSTPLLYACIAYETTILTETTLSPSSHASSLSSLALPKIDHSKPQKATYAREPYHIHYLASSASEYPDDEASAGRLTYLVISPDKTLYRVTCFEFLTTLRTRFLARFPPEKHDFSNFLPYGCAEFEGELRKLMAEYSQRALDVENNGGDSINSVQREIEDVRGIMSRNIEGLLERGERIDLLVDKTDRLGGSAREFRLRSRGLKRKMWWKNVKLMGLLGFVVVLILLTIVTSVKGGV DIP protein G0S5G3 original sequence version: 19 g0s236_chatd CTHT_0015850 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MSFDQLSSLEAGRRRGPTTSSRYTDDPEFARLSQDLMNKLFRLNGNNQRLAGEIGHLGTRRDTPRVRERVHELIEESRDMFKQVGEGVKKIQAWEDVTPTQRYMQQKLSREFQTSLAEFQSLQRQALEKQKASVSAARASFESEHHAPSATGAPEQHQLLQQQQELTHLAPQDEVDFQEALILEREEEIRNIEQGVSDLNVLFQQVAQLVAEQGEVLDTIERNVEAVGDDTRGADRELRAAARYQKRARSRMCCLLMILTVILTIILLAIFVG DIP protein G0S236 original sequence version: 18 g0scm5_chatd CTHT_0057760 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MAGQFAAKRLGKELQKIRNSLPPGIELVSADNFEEWLLDIRVLDDNPLYANQTYRLRFRFGSTYPIEPPEVVFVKSADRPIPIHPHIYSNGIICLDLLGHQGWSPVQNVESVCMSLQTPRLPTPTWIHNHQTPHGGLVSMAPRAGFDAEQVRDKARKDLLHLLEGVRGKKNLVIEKDLAGPLGVIVKASTLRDYGVDNFFFLENKNTGTSQRNIVFIARGESVRNAHAIAAQIKRIQRESQTSHDFHIFWVPRRTLFSDKVLEEAGVLGDANISELPLYFFPLERDVLSLELNDSFRDLYLAKDPTPVFLLSRALMGIQKKHGLFPRIIGKGENAKRVADLLSRMRQELLAGEEAGESDRAGLSPSTTIESVIIIDREVDFVTPLLTQLTYEGLIDEYFGIQNNQTDVDAVIVGAPAQSAASTSTAVPTNSSQSRKRKIQLDGSDSLYSQLRDANFAIVGSLLNTVARRLKSDYESRHNTKTTAELKEFVKKLPGYQAEQQSLKIHSNIAEEIINYTRTEIFNKLLEVQQNLAAGADPSSQFDSIEELVARDTPLPQVLRLLCLYSCISGGIKTKELDHFRRLVLQGYGHQHLLTLHNLERLQMFLSKSSPLASMITMSGSSGGPDQKTNYTYLRKQLRLIVDEVNEQDPNDIAYVYSGYAPLSIRLVQCVLQKQYLLSITKGSGTVIAAGPVAGGGAQGWKGFEEIVKHARGPTFDEIQKGEDKAVKARALLSGSSGDKKTVFVVFVGGITFTEIAALRFIAKQEEARRNIVICTTSIINGNRMMNAAIETATFEKTTVTTAAAQ DIP protein G0SCM5 original sequence version: 19 DIP protein G0SCM5 original sequence version: 26 g0s236_chatd-g0s5g3_chatd-1 1 11 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association g0s6m7_chatd-g0scm5_chatd-4 5 10 unspecified role unspecified role unspecified role unspecified role Region503-816 Region503-816 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0s236_chatd-g0scm5_chatd-1 8 12 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association Deletion of region 476-498 decreased the interaction rate/strength. g0s5g3_chatd-g0scm5_chatd-1 9 11 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0s236_chatd-g0s6m7_chatd-1 4 12 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 503-816 Region 503-816 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0s5g3_chatd-g0scm5_chatd-2 2 11 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain G0S5G3:p.Phe201Ala F201A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F201A'. Wed May 02 20:19:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe201ala' Tue Jun 30 17:02:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe201Ala' ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association g0s5g3_chatd-g0scm5_chatd-3 6 11 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain G0SCM5:p.Gly460Asp G460D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G D This feature has been corrected as a result of our quality control procedures. The original label was 'G460D'. Wed May 02 15:00:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly460asp' Tue Jun 30 19:49:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly460Asp' G0SCM5:p.Ala467His A467H mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A H This feature has been corrected as a result of our quality control procedures. The original label was 'A467H'. Wed May 02 18:58:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala467his' Tue Jun 30 17:37:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala467His' G0SCM5:p.Phe489Ala F489A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F489A'. Wed May 02 19:36:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe489ala' Tue Jun 30 18:30:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe489Ala' G0SCM5:p.Ser507Glu S507E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S E This feature has been corrected as a result of our quality control procedures. The original label was 'S507E'. Wed May 02 18:06:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser507glu' Tue Jun 30 17:24:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser507Glu' Region 476-498 Region 476-498 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association g0s236_chatd-g0scm5_chatd-2 7 12 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0s5g3_chatd-g0s6m7_chatd-1 3 10 unspecified role unspecified role unspecified role unspecified role Region 503-816 Region 503-816 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction