DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 2015 imex curation Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266262X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 2015 imex curation Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266259X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 2015 imex curation Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266258X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 2015 imex curation Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266257X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 2015 imex curation Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266260X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 2015 imex curation Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266261X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG traf6_human TNF receptor-associated factor 6 TRAF6 RNF85 Interleukin-1 signal transducer RING finger protein 85 E3 ubiquitin-protein ligase TRAF6 RING-type E3 ubiquitin transferase TRAF6 protein protein human Homo sapiens Human MSLLNCENSCGSSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV DIP protein Q9Y4K3 original sequence version: 154 DIP protein Q9Y4K3 original sequence version: 91 DIP protein Q9Y4K3 original sequence version: 114 DIP protein Q9Y4K3 original sequence version: 118 DIP protein Q9Y4K3 original sequence version: 140 DIP protein Q9Y4K3 original sequence version: 159 DIP protein Q9Y4K3 original sequence version: 132 DIP protein Q9Y4K3 original sequence version: 158 DIP protein Q9Y4K3 original sequence version: 171 DIP protein Q9Y4K3 original sequence version: 117 DIP protein Q9Y4K3 original sequence version: 113 5AB9C255CCFEE749 tifa_human TRAF-interacting protein with FHA domain-containing protein A Putative NF-kappa-B-activating protein 20 TIFA T2BP TRAF2-binding protein Putative MAPK-activating protein PM14 protein protein human Homo sapiens Human MTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQVSRVQFSLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQFILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTEMDENES DIP protein Q96CG3 original sequence version: 104 2E7FE091A52415B0 tifa-traf6-2 4 8 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association tifa-traf6-4 2 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association traf6-ub-3 6 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association tifa-traf6-5 3 8 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96CG3:p.Thr9Ala T9A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T9A'. Wed May 02 16:34:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr9ala' Tue Jun 30 18:58:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr9Ala' Q96CG3:p.[Gly50Glu;Ser66Ala] G50E/S66A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain G E This feature has been corrected as a result of our quality control procedures. The original label was 'G50E/S66A'. Wed May 02 15:07:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly50glu,ser66ala' Tue Jun 30 19:43:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly50Glu;Ser66Ala]' Q96CG3:p.Glu178Ala E178A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E178A'. Wed May 02 17:10:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu178ala' Tue Jun 30 16:18:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu178Ala' human Homo sapiens Human physical association physical association tifa-ub-1 1 7 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association traf6-tifa-2 5 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait human Homo sapiens Human 9 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association