DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Science (New York, N.Y.) 2015 imex curation Kling A., Lukat P., Almeida DV., Bauer A., Fontaine E., Sordello S., Zaburannyi N., Herrmann J., Wenzel SC., König C., Ammerman NC., Barrio MB., Borchers K., Bordon-Pallier F., Brönstrup M., Courtemanche G., Gerlitz M., Geslin M., Hammann P., Heinz DW., Hoffmann H., Klieber S., Kohlmann M., Kurz M., Lair C., Matter H., Nuermberger E., Tyagi S., Fraisse L., Grosset JH., Lagrange S., Müller R. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kling A., Lukat P., Almeida DV., Bauer A., Fontaine E., Sordello S., Zaburannyi N., Herrmann J., Wenzel SC., König C., Ammerman NC., Barrio MB., Borchers K., Bordon-Pallier F., Brönstrup M., Courtemanche G., Gerlitz M., Geslin M., Hammann P., Heinz DW., Hoffmann H., Klieber S., Kohlmann M., Kurz M., Lair C., Matter H., Nuermberger E., Tyagi S., Fraisse L., Grosset JH., Lagrange S., Müller R. DIP original experiment: DIP-266253X Science (New York, N.Y.) 2015 IMEx Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Science (New York, N.Y.) 2015 imex curation Kling A., Lukat P., Almeida DV., Bauer A., Fontaine E., Sordello S., Zaburannyi N., Herrmann J., Wenzel SC., König C., Ammerman NC., Barrio MB., Borchers K., Bordon-Pallier F., Brönstrup M., Courtemanche G., Gerlitz M., Geslin M., Hammann P., Heinz DW., Hoffmann H., Klieber S., Kohlmann M., Kurz M., Lair C., Matter H., Nuermberger E., Tyagi S., Fraisse L., Grosset JH., Lagrange S., Müller R. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kling A., Lukat P., Almeida DV., Bauer A., Fontaine E., Sordello S., Zaburannyi N., Herrmann J., Wenzel SC., König C., Ammerman NC., Barrio MB., Borchers K., Bordon-Pallier F., Brönstrup M., Courtemanche G., Gerlitz M., Geslin M., Hammann P., Heinz DW., Hoffmann H., Klieber S., Kohlmann M., Kurz M., Lair C., Matter H., Nuermberger E., Tyagi S., Fraisse L., Grosset JH., Lagrange S., Müller R. DIP original experiment: DIP-266254X Science (New York, N.Y.) 2015 IMEx Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. Science (New York, N.Y.) 2015 imex curation Kling A., Lukat P., Almeida DV., Bauer A., Fontaine E., Sordello S., Zaburannyi N., Herrmann J., Wenzel SC., König C., Ammerman NC., Barrio MB., Borchers K., Bordon-Pallier F., Brönstrup M., Courtemanche G., Gerlitz M., Geslin M., Hammann P., Heinz DW., Hoffmann H., Klieber S., Kohlmann M., Kurz M., Lair C., Matter H., Nuermberger E., Tyagi S., Fraisse L., Grosset JH., Lagrange S., Müller R. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kling A., Lukat P., Almeida DV., Bauer A., Fontaine E., Sordello S., Zaburannyi N., Herrmann J., Wenzel SC., König C., Ammerman NC., Barrio MB., Borchers K., Bordon-Pallier F., Brönstrup M., Courtemanche G., Gerlitz M., Geslin M., Hammann P., Heinz DW., Hoffmann H., Klieber S., Kohlmann M., Kurz M., Lair C., Matter H., Nuermberger E., Tyagi S., Fraisse L., Grosset JH., Lagrange S., Müller R. DIP original experiment: DIP-266255X Science (New York, N.Y.) 2015 IMEx Antibiotics. Targeting DnaN for tuberculosis therapy using novel griselimycins. a0qx55_mycs2 DNA-directed DNA polymerase MSMEG_3178 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSGTDGRSSGSFVHLHNHTEYSMLDGAAKITPMLAEAQRLEMPAIGMTDHGNMFGASEFYNSATKAGIKPIIGIEAYIAPGSRFDTKRVTWGDPGQKGDDVSGSGAYTHMTMVAENATGLRNLFKLSSLASFEGQLGKWSRMDAEIIAEHAEGIIATTGCPSGEVQTRLRLGHEREALEAAAKWREIFGPENFFLELMDHGLDIERRVREGLLEIGRKLGIPPLATNDCHYVTREAARNHEALLCVQTGKTLSDPTRFKFDGDGYFLKSAEEMRALWDSQVPGACDSTLLIGERVQSYADVWEPRDRMPVFPVPEGHDQASWLTHEVKAGLERRFRGGPVPEEYTTRAEYEIKVICDKGFPSYFLIVADLINYARSVGIRVGPGRGSAAGSLVAYALGITNIDPIPHGLLFERFLNPERPSAPDIDIDFDDRRRGEMLRYAANRWGSERVAQVITFGTIKTKAALKDSARVNFGQPGFAIADRITKALPPPIMAKDIPLSGITDPNHERYKEAAEVRGLIDTDPDVRTIYETARGLEGLVRNAGVHACAVIMSSEPLIDAIPLWKRPQDGAIITGWDYPSCEAIGLLKMDFLGLRNLTIIGDAIANIKANRGIDLDLETLPLDDPAAYELLSRGDTLGVFQLDGGPMRDLLRRMQPTGFNDIVAVLALYRPGPMGMNAHNDYADRKNGRQPIKPIHPELEEPLKDILAETYGLIVYQEQIMFIAQKVASYTMGKADALRKAMGKKKLEVLEAEYKGFKEGMTANGFSEGAVKALWDTILPFAGYAFNKSHAAGYGLVSYWTAYLKANYPAEYMAGLLTSVGDDKDKAAVYLADCRRLGITVLPPDVNESEHNFASVGDDIRFGLGAIRNVGANVVSSLIKTRTEKGKFTDFSDYLNKIDITACNKKVTESLIKAGAFDSLGHPRKGLFLVHTDAVDSVLGTKKAEAMGQFDLFGGGEDTGTDAVFTIKVPDEEWEDKHKLALEREMLGLYVSGHPLNGVAHLLANQVDTQIPAILDGDVANDAQVLVGGILASVNRRVNKNGLPWASAQLEDLTGGIEVLFFPQTYSLFGADIADDVVVLVKAKVAARDDRIALIAHELIVPDFSSAQADRPLSVSLPTRQCTIDKVTALKQVLANHPGTSQVHLRLISGERITTLELDQSLRVTPSSALMGDLKALLGPGCLG DIP protein A0QX55 original sequence version: 70 dpo3b_myctu Beta sliding clamp Beta-clamp processivity factor DNA polymerase III beta sliding clamp subunit DNA polymerase III subunit beta Mtb beta-clamp MTCY10H4.0 MTV029.02 Rv0002 dnaN protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MDAATTRVGLTDLTFRLLRESFADAVSWVAKNLPARPAVPVLSGVLLTGSDNGLTISGFDYEVSAEAQVGAEIVSPGSVLVSGRLLSDITRALPNKPVDVHVEGNRVALTCGNARFSLPTMPVEDYPTLPTLPEETGLLPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILGETVVLAATDRFRLAVRELKWSASSPDIEAAVLVPAKTLAEAAKAGIGGSDVRLSLGTGPGVGKDGLLGISGNGKRSTTRLLDAEFPKFRQLLPTEHTAVATMDVAELIEAIKLVALVADRGAQVRMEFADGSVRLSAGADDVGRAEEDLVVDYAGEPLTIAFNPTYLTDGLSSLRSERVSFGFTTAGKPALLRPVSGDDRPVAGLNGNGPFPAVSTDYVYLLMPVRLPG 1AB4B6D18653B42D dpo3b_mycs2 Beta sliding clamp Beta-clamp processivity factor DNA polymerase III beta sliding clamp subunit DNA polymerase III subunit beta MSMEG_0001 MSMEI_0003 dnaN protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MATTTAGLTDLKFRVVREDFADAVAWVARSLPTRPTIPVLAGVLLTGTDEGLTISGFDYEVSAEVKVSAEIASAGSVLVSGRLLSDITKALPAKPVEVSVEGTRVSLTCGSARFSLPTLAVEDYPALPALPEETGVIASDLFAEAIGQVAVAAGRDDTLPMLTGIRVEISGESVVLAATDRFRLAVRELTWVTTAGDVEAAVLVPAKTLAEAAKAGTDGNQVHLALGSGASVGKDGLLGIRSEGKRSTTRLLDAEFPKFRQLLPAEHTAVATIGVAELTEAIKRVALVADRGAQIRMEFSDDTLKLSAGADDVGRAEEDLPVDFAGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPSRPAVLRPAGEDDGANGGSGPFPAAKTDYVYLLMPVRLPG DIP protein A0QND6 original sequence version: 74 dnan-10 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction i6xu56_myctu-1 2 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction dnan-dnae1-1 3 4 unspecified role unspecified role prey prey Region 943-954 Region 943-954 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction