DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. Nature structural & molecular biology 2015 imex curation Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. DIP original experiment: DIP-266061X Nature structural & molecular biology 2015 IMEx An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. Nature structural & molecular biology 2015 imex curation Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. DIP original experiment: DIP-266062X Nature structural & molecular biology 2015 IMEx An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. Nature structural & molecular biology 2015 imex curation Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. DIP original experiment: DIP-266064X Nature structural & molecular biology 2015 IMEx An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. An ancient protein-DNA interaction underlying metazoan sex determination. Nature structural & molecular biology 2015 imex curation Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Murphy MW., Lee JK., Rojo S., Gearhart MD., Kurahashi K., Banerjee S., Loeuille GA., Bashamboo A., McElreavey K., Zarkower D., Aihara H., Bardwell VJ. DIP original experiment: DIP-266063X Nature structural & molecular biology 2015 IMEx An ancient protein-DNA interaction underlying metazoan sex determination. dmrt1_human Doublesex- and mab-3-related transcription factor 1 DMRT1 DMT1 DM domain expressed in testis protein 1 protein protein human Homo sapiens Human MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKASGALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMENRHAMSSQYRMHSYYPPPSYLGQSVPQFFTFEDAPSYPEARASVFSPPSSQDSGLVSLSSSSPISNKSTKAVLECEPASEPSSFTVTPVIEEDE DIP protein Q9Y5R6 original sequence version: 123 mint 23348B2C4A4733A1 dmrt1-1 1 5 unspecified role unspecified role unspecified role unspecified role Region 67-136 Region 67-136 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dmrt1-2 2 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction dmrt1-3 4 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro physical association physical association dmrt1-4 3 5 unspecified role unspecified role unspecified role unspecified role Region 67-136 Region 67-136 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association