DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structure (London, England : 1993) 2015 imex curation Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. DIP original experiment: DIP-265781X Structure (London, England : 1993) 2015 IMEx Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structure (London, England : 1993) 2015 imex curation Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. DIP original experiment: DIP-265779X Structure (London, England : 1993) 2015 IMEx Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structure (London, England : 1993) 2015 imex curation Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. DIP original experiment: DIP-265780X Structure (London, England : 1993) 2015 IMEx Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. Structure (London, England : 1993) 2015 imex curation Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wang X., Hou Y., Deng K., Zhang Y., Wang DC., Ding J. DIP original experiment: DIP-265778X Structure (London, England : 1993) 2015 IMEx Structural Insights into the Molecular Recognition between Cerebral Cavernous Malformation 2 and Mitogen-Activated Protein Kinase Kinase Kinase 3. m3k3_human Mitogen-activated protein kinase kinase kinase 3 MAP3K3 MAPKKK3 MEKK3 MAPK/ERK kinase kinase 3 protein protein human Homo sapiens Human MDEQEALNSIMNDLVALQMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY DIP protein Q99759 original sequence version: 164 DIP protein Q99759-1 original sequence version: 173 28129168A57571DD q9bsq5-1 Cerebral cavernous malformations 2 protein CCM2 C7orf22 Malcavernin PP10187 protein protein human Homo sapiens Human MEEEGKKGKKPGIVSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLPERVEPDRLLSDYIEKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATELLQDYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTTNGNRATGSSDDRSAPSEGDEWDRMISDISSDIEALGCSMDQDSA DIP protein Q9BSQ5-1 original sequence version: 117 DIP protein Q9BSQ5-1 original sequence version: 125 ccm2-map3k3-1 4 5 unspecified role unspecified role prey prey Region 1-22 Region 1-22 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role bait bait Region 290-376 Region 290-376 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ccm2-map3k3-2 3 5 unspecified role unspecified role prey prey Region 1-126 Region 1-126 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 290-376 Region 290-376 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction ccm2-map3k3-3 2 5 unspecified role unspecified role prey prey Region 1-126 Region 1-126 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99759:p.Leu7Asp L7D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L7D'. Wed May 02 19:30:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu7asp' Tue Jun 30 16:14:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu7Asp' Q99759:p.Ile10Asp I10D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I10D'. Wed May 02 14:32:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile10asp' Tue Jun 30 19:31:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile10Asp' Q99759:p.Leu14Asp L14D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L14D'. Wed May 02 17:18:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu14asp' Tue Jun 30 18:54:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu14Asp' Q99759:p.Leu17Asp L17D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L17D'. Wed May 02 14:58:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu17asp' Tue Jun 30 17:37:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu17Asp' ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 290-376 Region 290-376 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ccm2-map3k3-4 1 5 unspecified role unspecified role unspecified role unspecified role Region 1-22 Region 1-22 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role Region 290-376 Region 290-376 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction