UniProt UniProt http://www.uniprot.org RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD Cell reports 2015 imex curation RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD Cell reports 2015 imex curation RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD Cell reports 2015 imex curation RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel anti tag western anti tag western blot anti tag western Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD Cell reports 2015 imex curation RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD Cell reports 2015 imex curation RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD human lung cDNA Cell reports 2015 imex curation RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell reports 2015 imex curation Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel anti tag western anti tag western blot anti tag western Takashima K., Oshiumi H., Takaki H., Matsumoto M., Seya T. seya-tu@pop.med.hokudai.ac.jp Accepted 2017-FEB-22 AT 11:17 GMT AT 11:17 GMT by ORCHARD Cell reports 2015 imex curation riok3_human Serine/threonine-protein kinase RIO3 sudD homolog RIOK3 SUDD RIO kinase 3 protein protein human Homo sapiens Human MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE 3C3392BAC4ECB912 ifih1_human Interferon-induced helicase C domain-containing protein 1 Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated protein 5 Murabutide down-regulated protein RNA helicase-DEAD box protein 116 RIG-I-like receptor 2 IFIH1 MDA5 RH116 Clinically amyopathic dermatomyositis autoantigen 140 kDa protein protein human Homo sapiens Human MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENSLLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTASPGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTRIQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED DIP protein Q9BYX4 original sequence version: 130 DIP protein Q9BYX4 original sequence version: 120 DIP protein Q9BYX4 original sequence version: 123 DIP protein Q9BYX4 original sequence version: 66 DIP protein Q9BYX4 original sequence version: 82 DIP protein Q9BYX4-1 original sequence version: 136 DIP protein Q9BYX4-1 original sequence version: 107 DIP protein Q9BYX4 original sequence version: 122 789CFB4824B92DC9 ifih1-riok3-1 6 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 12 physical association physical association no figure ifih1-riok3-2 5 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association 1D ifih1-riok3-3 2 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1E, F riok3-ifih1-1 3 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1G Observed before and after polyI:C stimulation ifih1-riok3-4 1 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 1K ifih1-ifih1-2 4 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BYX4:p.Ser828Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:18:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser828ala' Tue Jun 30 20:15:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser828Ala' Q9BYX4:p.Ser828Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 19:43:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser828asp' Tue Jun 30 15:37:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser828Asp' 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BYX4:p.Ser828Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:16:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser828ala' Tue Jun 30 17:40:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser828Ala' Q9BYX4:p.Ser828Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 19:46:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser828asp' Tue Jun 30 17:47:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser828Asp' physical association physical association 4B-E, S4A-B Multinerization observed in resting and polyI:C stimulated cells ifih1-ifih1-1 7 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4F-I Multinerization observed in resting and polyI:C stimulated cells RIOK3. RIOK3 knock-out increases multimer formation ifih1-riok3-5 2 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association S1C