DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure (London, England : 1993) 2015 imex curation Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. DIP original experiment: DIP-264829X Structure (London, England : 1993) 2015 IMEx DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure (London, England : 1993) 2015 imex curation Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. DIP original experiment: DIP-264828X Structure (London, England : 1993) 2015 IMEx DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure (London, England : 1993) 2015 imex curation Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. DIP original experiment: DIP-264830X Structure (London, England : 1993) 2015 IMEx DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure (London, England : 1993) 2015 imex curation Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. DIP original experiment: DIP-264832X Structure (London, England : 1993) 2015 IMEx DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure (London, England : 1993) 2015 imex curation Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. DIP original experiment: DIP-264831X Structure (London, England : 1993) 2015 IMEx DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure (London, England : 1993) 2015 imex curation Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. Only protein-protein interactions in vitro In vitro sans small angle neutron scattering SANS SANS sans predetermined predetermined participant predetermined Nowicka U., Zhang D., Walker O., Krutauz D., Castañeda CA., Chaturvedi A., Chen TY., Reis N., Glickman MH., Fushman D. DIP original experiment: DIP-264833X Structure (London, England : 1993) 2015 IMEx DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. yub Ubiquitin protein protein yeast Saccharomyces cerevisiae Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG This sequence has been withdrawn from Uniprot. ddi1_yeast DNA damage-inducible protein 1 DDI1 v-SNARE-master 1 VSM1 YER143W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDLTISNELTGEIYGPIEVSEDMALTDLIALLQADCGFDKTKHDLYYNMDILDSNRTQSLKELGLKTDDLLLIRGKISNSIQTDAATLSDEAFIEQFRQELLNNQMLRSQLILQIPGLNDLVNDPLLFRERLGPLILQRRYGGYNTAMNPFGIPQDEYTRLMANPDDPDNKKRIAELLDQQAIDEQLRNAIEYTPEMFTQVPMLYINIEINNYPVKAFVDTGAQTTIMSTRLAKKTGLSRMIDKRFIGEARGVGTGKIIGRIHQAQVKIETQYIPCSFTVLDTDIDVLIGLDMLKRHLACVDLKENVLRIAEVETSFLSEAEIPKSFQEGLPAPTSVTTSSDKPLTPTKTSSTLPPQPGAVPALAPRTGMGPTPTGRSTAGATTATGRTFPEQTIKQLMDLGFPRDAVVKALKQTNGNAEFAASLLFQ B43425B029B7658B B43425B029B7658B ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ufo1_yeast Ubiquitin ligase complex F-box protein UFO1 UFO1 YML088W protein protein yeast Saccharomyces cerevisiae Baker's yeast MERPGLVLQDLPPEILINIFSHLDEKDLFTLQELSTHFRNLIHDEELWKNLFKSRVHTTHFPTFSQSSKFSVEYIERTRGLHHWQHNKAIRTKYTIIPTRNWDQPSIERIVFDYPRVAAYNDGTITILQLQNHKRQKKFKKLIYIPCTTPQGCSTMDFNINAAVFGRFDGRVFGKLLSNKSYLTPVMEFTGRHSAGVTAICNSESWDTSREDWSVSGSENGEIIWWCENKLVKMWKVSNRVIWKLAFFKDWTLIMDDEKLYIIHQMQELHSIDIPKDLDEQPMRVRFFKMDFGSMTLVLADLNNVYTISVNPNGNFGNLRKLEMPEQICAVEIDEKTSQREQNWQFAGDDGCYISLLTTQNTLYIINIRDLSSSGLKVQCKISFDEQVYVSQVTNLIVVVALPNVLQILNAMTGELIKTVLKTEKFPEFLKVSQDKIIMGSGNVLNYLKFVSSDSKKHHHSTKGKNTVSNKWNETLNTELQYYDEDEDLRRKRQSEISRLIDAYGGDLELSGDTDEENDIQLRIALLESQEAQARNQAEAGEPVGDDEDEQLRRALEESQLIYETQTNSSANHGNNTNDEIDEDDEEFLRAIRQSRVEDERRRHLRNHTTGRRNGPLSDDNFATYGAAESSERTSTENTIGSSVGVDASNNVDEDLQLAIALSLSEIN 10CA467CF9417FBC 10CA467CF9417FBC hub-ddi1-1 3 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 8 unspecified role unspecified role prey prey Region 2-80 Region 2-80 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction hub-ufo1-1 4 10 unspecified role unspecified role unspecified role unspecified role Region 547-668 Region 547-668 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ddi1-hub-1 2 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 389-428 Region 389-428 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ddi1-hub-2 1 9 unspecified role unspecified role unspecified role unspecified role lys6ala,arg42ala,arg72ala K6A/R42A/R72A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'K6A/R42A/R72A'. lys6glu,arg42glu,arg72glu K6E/R42E/R72E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'K6E/R42E/R72E'. ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 2-80 Region 2-80 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ddi1-yub-1 5 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait Region 2-80 Region 2-80 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association ddi1-hub-3 6 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 2-80 Region 2-80 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction