DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264783X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264786X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264798X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264784X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264795X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264791X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264796X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264787X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264789X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264788X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264785X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264793X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264790X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264794X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264797X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. Structure (London, England : 1993) 2015 imex curation Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Lakomek K., Stoehr G., Tosi A., Schmailzl M., Hopfner KP. DIP original experiment: DIP-264792X Structure (London, England : 1993) 2015 IMEx Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2. g0ryi5_chatd RuvB-like helicase CTHT_0006820 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MVQISEVRGNTRDHRTAAHTHIKGLGLNSSGIAEKQAAGFVGQCAAREACGVVVDLIKAHKMAGRGVLLAGGPGTGKTALALAISQELGTKIPFCPITGSEIYSTEVKKTEVLMENFRRAIGLRVRETKDVYEGEVTEMTPEEAENPLGGYGKTISTLLIGLKSARGQKKLRLDPSIYEAIQKERVQVGDVIYIETNTGACKRVGRSDAYATEFDLEAEEYVPIPKGEVHKKKEIVQDVTLHDLDVANARPQGGQDIISMMGQLMKPKMTEITDKLRMEINKVVQKYINQGVAELIPGVLFIDEAHMLDIECFTYLNKALESPIAPIVVLASNRGIATIRGADDLKAAHGIPPDFLQRLLIIPTHPYEPDEIRRIVRIRAQTEGVQLTDAAVDRVAEHGVRISLRYCLQLLAPASILARVNGRTQVDVQDIAEAEELFLDARRSANILTSTGESGGLHGFIS DIP protein G0RYI5 original sequence version: 17 g0ryc2_chatd RuvB-like helicase CTHT_0006170 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MAAPLVTSVTETKELRGLNLIAAHSHIRGLGVDADTLEPRPSSQGLVGQEKARKAAAVVLEMIKQGKIAGRAVLIAGPPSTGKTAIAMGMAQSLGQDVPFTTLAASEIFSLEMSKTEALTQAFRKSIGVRIKEESEIMEGEVVEIQIDRSVTGGAKQGKLTIKTTDMEAIYDMGSKMIDAMTKERVMAGDIISIDKSSGKITKLGRSYARSRDYDAMGVDTKFLQCPEGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIRSEIRDQINTKVAEWKEEGKAEIVPGVLFIDEVHMLDIECFSYINRALESDLAPIVIMASNRGVSRIRGTDYKSPHGLPLDFLDRVVIINTHPYTPDELRQILSIRAQEEEVDLTPDALALLTKIGQEAGLRYASNLITTSQLIAAKRRAKQVGVEDVQRSFKLFYDPARSVRFVQESEKRLIGNDGVVDFSYQGAAEAAAPTLPAAAPVDPVGGEKMDMS DIP protein G0RYC2 original sequence version: 17 g0ryc2_chatd-g0ryi5_chatd-1 12 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-2 1 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-3 15 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-4 4 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-5 9 18 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 17 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association g0ryc2_chatd-g0ryi5_chatd-6 14 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-7 3 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-8 11 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-9 13 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-10 5 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-11 2 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-12 6 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-13 8 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-14 7 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-15 10 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryc2_chatd-g0ryi5_chatd-16 16 18 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role CL-K CL-K xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction