IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Allosteric activation of ADAMTS13 by von Willebrand factor. Allosteric activation of ADAMTS13 by von Willebrand factor. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay tag fluorescence tag visualisation by fluorescence tag fluorescence Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. Cleavage was detected as an increase in fluorescence esadler@dom.wustl.edu Accepted 2017-MAR-08 AT 13:40 GMT AT 13:40 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Allosteric activation of ADAMTS13 by von Willebrand factor. Allosteric activation of ADAMTS13 by von Willebrand factor. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. Plasma VWF was vortexed (with ADAMTS13 variants) to imitate fluid shear stress and start cleavage of multimeric VWF. esadler@dom.wustl.edu Accepted 2017-MAR-08 AT 13:40 GMT AT 13:40 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Allosteric activation of ADAMTS13 by von Willebrand factor. Allosteric activation of ADAMTS13 by von Willebrand factor. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu Accepted 2017-MAR-08 AT 13:40 GMT AT 13:40 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Allosteric activation of ADAMTS13 by von Willebrand factor. Allosteric activation of ADAMTS13 by von Willebrand factor. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay weight by staining molecular weight estimation by staining weight by staining Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu Accepted 2017-MAR-08 AT 13:40 GMT AT 13:40 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation ats13_human A disintegrin and metalloproteinase with thrombospondin motifs 13 von Willebrand factor-cleaving protease ADAMTS13 C9orf8 UNQ6102/PRO20085 protein protein human Homo sapiens Human MHQRHPRARCPPLCVAGILACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSPGAPLKGRPPSPGFQRQRQRQRRAAGGILHLELLVAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPSLGAQFRVHLVKMVILTEPEGAPNITANLTSSLLSVCGWSQTINPEDDTDPGHADLVLYITRFDLELPDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLGVTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASDGAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDPPRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKAVACTFAREHLDMCQALSCHTDPLDQSSCSRLLVPLLDGTECGVEKWCSKGRCRSLVELTPIAAVHGRWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFGGRACVGADLQAEMCNTQACEKTQLEFMSQQCARTDGQPLRSSPGGASFYHWGAAVPHSQGDALCRHMCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTLSLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDNSTCSPRKGSFTAGRAREYVTFLTVTPNLTSVYIANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPSLLEDGRVEYRVALTEDRLPRLEEIRIWGPLQEDADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQAWVWAAVRGPCSVSCGAGLRWVNYSCLDQARKELVETVQCQGSQQPPAWPEACVLEPCPPYWAVGDFGPCSASCGGGLRERPVRCVEAQGSLLKTLPPARCRAGAQQPAVALETCNPQPCPARWEVSEPSSCTSAGGAGLALENETCVPGADGLEAPVTEGPGSVDEKLPAPEPCVGMSCPPGWGHLDATSAGEKAPSPWGSIRTGAQAAHVWTPAAGSCSVSCGRGLMELRFLCMDSALRVPVQEELCGLASKPGSRREVCQAVPCPARWQYKLAACSVSCGRGVVRRILYCARAHGEDDGEEILLDTQCQGLPRPEPQEACSLEPCPPRWKVMSLGPCSASCGLGTARRSVACVQLDQGQDVEVDEAACAALVRPEASVPCLIADCTYRWHVGTWMECSVSCGDGIQRRRDTCLGPQAQAPVPADFCQHLPKPVTVRGCWAGPCVGQGTPSLVPHEEAAAPGRTTATPAGASLEWSQARGLLFSPAPQPRRLLPGPQENSVQSSACGRQHLEPTGTIDMRGPGQADCAVAIGRPLGEVVTLRVLESSLNCSAGDMLLLWGRLTWRKMCRKLLDMTFSSKTNTLVVRQRCGRPGGGVLLRYGSQLAPETFYRECDMQLFGPWGEIVSPSLSPATSNAGGCRLFINVAPHARIAIHALATNMGAGTEGANASYILIRDTHSLRTTAFHGQQVLYWESESSQAEMEFSEGFLKAQASLRGQYWTLQSWVPEMQDPQSWKGKEGT DIP protein Q76LX8 original sequence version: 50 A2103AFABC1A4445 sspa_staau Glutamyl endopeptidase sspA Endoproteinase Glu-C Staphylococcal serine proteinase V8 protease V8 proteinase protein protein staau Staphylococcus aureus MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA 8B138D0C7996AA3E vwf_human von Willebrand factor VWF F8VWF protein protein human Homo sapiens Human MIPARFAGVLLALALILPGTLCAEGTRGRSSTARCSLFGSDFVNTFDGSMYSFAGYCSYLLAGGCQKRSFSIIGDFQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYASKGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWALSSGEQWCERASPPSSSCNISSGEMQKGLWEQCQLLKSTSVFARCHPLVDPEPFVALCEKTLCECAGGLECACPALLEYARTCAQEGMVLYGWTDHSACSPVCPAGMEYRQCVSPCARTCQSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVHSGKRYPPGTSLSRDCNTCICRNSQWICSNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCQDHSFSIVIETVQCADDRDAVCTRSVTVRLPGLHNSLVKLKHGAGVAMDGQDVQLPLLKGDLRIQHTVTASVRLSYGEDLQMDWDGRGRLLVKLSPVYAGKTCGLCGNYNGNQGDDFLTPSGLAEPRVEDFGNAWKLHGDCQDLQKQHSDPCALNPRMTRFSEEACAVLTSPTFEACHRAVSPLPYLRNCRYDVCSCSDGRECLCGALASYAAACAGRGVRVAWREPGRCELNCPKGQVYLQCGTPCNLTCRSLSYPDEECNEACLEGCFCPPGLYMDERGDCVPKAQCPCYYDGEIFQPEDIFSDHHTMCYCEDGFMHCTMSGVPGSLLPDAVLSSPLSHRSKRSLSCRPPMVKLVCPADNLRAEGLECTKTCQNYDLECMSMGCVSGCLCPPGMVRHENRCVALERCPCFHQGKEYAPGETVKIGCNTCVCQDRKWNCTDHVCDATCSTIGMAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFRILVGNKGCSHPSVKCKKRVTILVEGGEIELFDGEVNVKRPMKDETHFEVVESGRYIILLLGKALSVVWDRHLSISVVLKQTYQEKVCGLCGNFDGIQNNDLTSSNLQVEEDPVDFGNSWKVSSQCADTRKVPLDSSPATCHNNIMKQTMVDSSCRILTSDVFQDCNKLVDPEPYLDVCIYDTCSCESIGDCACFCDTIAAYAHVCAQHGKVVTWRTATLCPQSCEERNLRENGYECEWRYNSCAPACQVTCQHPEPLACPVQCVEGCHAHCPPGKILDELLQTCVDPEDCPVCEVAGRRFASGKKVTLNPSDPEHCQICHCDVVNLTCEACQEPGGLVVPPTDAPVSPTTLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMMERLRISQKWVRVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKYTLFQIFSKIDRPEASRITLLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHANLKQIRLIEKQAPENKAFVLSSVDELEQQRDEIVSYLCDLAPEAPPPTLPPDMAQVTVGPGLLGVSTLGPKRNSMVLDVAFVLEGSDKIGEADFNRSKEFMEEVIQRMDVGQDSIHVTVLQYSYMVTVEYPFSEAQSKGDILQRVREIRYQGGNRTNTGLALRYLSDHSFLVSQGDREQAPNLVYMVTGNPASDEIKRLPGDIQVVPIGVGPNANVQELERIGWPNAPILIQDFETLPREAPDLVLQRCCSGEGLQIPTLSPAPDCSQPLDVILLLDGSSSFPASYFDEMKSFAKAFISKANIGPRLTQVSVLQYGSITTIDVPWNVVPEKAHLLSLVDVMQREGGPSQIGDALGFAVRYLTSEMHGARPGASKAVVILVTDVSVDSVDAAADAARSNRVTVFPIGIGDRYDAAQLRILAGPAGDSNVVKLQRIEDLPTMVTLGNSFLHKLCSGFVRICMDEDGNEKRPGDVWTLPDQCHTVTCQPDGQTLLKSHRVNCDRGLRPSCPNSQSPVKVEETCGCRWTCPCVCTGSSTRHIVTFDGQNFKLTGSCSYVLFQNKEQDLEVILHNGACSPGARQGCMKSIEVKHSALSVELHSDMEVTVNGRLVSVPYVGGNMEVNVYGAIMHEVRFNHLGHIFTFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQRPGQTCQPILEEQCLVPDSSHCQVLLLPLFAECHKVLAPATFYAICQQDSCHQEQVCEVIASYAHLCRTNGVCVDWRTPDFCAMSCPPSLVYNHCEHGCPRHCDGNVSSCGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQPCQICTCLSGRKVNCTTQPCPTAKAPTCGLCEVARLRQNADQCCPEYECVCDPVSCDLPPVPHCERGLQPTLTNPGECRPNFTCACRKEECKRVSPPSCPPHRLPTLRKTQCCDEYECACNCVNSTVSCPLGYLASTATNDCGCTTTTCLPDKVCVHRSTIYPVGQFWEEGCDVCTCTDMEDAVMGLRVAQCSQKPCEDSCRSGFTYVLHEGECCGRCLPSACEVVTGSPRGDSQSSWKSVGSQWASPENPCLINECVRVKEEVFIQQRNVSCPQLEVPVCPSGFQLSCKTSACCPSCRCERMEACMLNGTVIGPGKTVMIDVCTTCRCMVQVGVISGFKLECRKTTCNPCPLGYKEENNTGECCGRCLPTACTIQLRGGQIMTLKRDETLQDGCDTHFCKVNERGEYFWEKRVTGCPPFDEHKCLAEGGKIMKIPGTCCDTCEEPECNDITARLQYVKVGSCKSEVEVDIHYCQGKCASKAMYSIDINDVQDQCSCCSPTRTEPMQVALHCTNGSVVYHEVLNAMECKCSPRKCSK DIP protein P04275 original sequence version: 134 DIP protein P04275 original sequence version: 127 DIP protein P04275 original sequence version: 133 D5C1C78360917C29 adamts13-vwf-2 1 5 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain N C Wed May 02 15:13:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1610cys' Tue Jun 30 17:48:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1610Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain E G Wed May 02 15:29:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1598gly' Tue Jun 30 20:10:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1598Gly' P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed May 02 14:42:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1617arg' Tue Jun 30 17:46:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1617Arg' IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 1, Supp 1 increase in pH, but only for full length version of ADAMTS13 Full length ADAMTS13 showed maximum activity at pH6 and decreased activity at pH 7.4. The MDTCS construct (ADAMTS13 aas 1-684) maintained maximal activity at all pH values tested. adamts13-vwf-3 1 5 enzyme enzyme neutral component neutral component MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain N C Wed May 02 14:51:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1610cys' Tue Jun 30 19:29:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1610Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain E G Wed May 02 14:53:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1598gly' Tue Jun 30 18:41:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1598Gly' P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed May 02 20:16:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1617arg' Tue Jun 30 18:33:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1617Arg' IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 24 19 protein cleavage protein cleavage 1D, supp 1 increase in pH, but only for full length version of ADAMTS13 Full length ADAMTS13 showed maximum activity at pH6 and decreased activity at pH 7.4. The MDTCS construct (ADAMTS13 aas 1-684) maintained maximal activity at all pH values tested. adamts13-vwf-4 1 7 stimulator stimulator neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 7 stimulator stimulator neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 5 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain N C Wed May 02 17:56:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1610cys' Tue Jun 30 16:58:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1610Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain E G Wed May 02 18:02:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1598gly' Tue Jun 30 20:14:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1598Gly' P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed May 02 15:51:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1617arg' Tue Jun 30 17:55:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1617Arg' IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 31 33 protein cleavage protein cleavage 2D adamts13-vwf-5 1 5 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain N C Wed May 02 16:32:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1610cys' Tue Jun 30 19:09:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1610Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain E G Wed May 02 20:28:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1598gly' Tue Jun 30 16:27:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1598Gly' P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Wed May 02 18:16:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1617arg' Tue Jun 30 19:53:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1617Arg' IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 stimulator stimulator neutral component neutral component D4_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage 2D Adding VWF cleavage fragments had little effect on ADAMTS13 activity. adamts13-vwf-6 2 5 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 7 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 58 60 protein cleavage protein cleavage 3 full-length ADAMTS13 cleaved VWF ∼1.7-fold faster than MDTCS. adamts13-vwf-10 4 6 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 7 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 64 66 protein cleavage protein cleavage Supp 3 adamts13-vwf-11 2 5 enzyme enzyme neutral component neutral component MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 7 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-plasma homo sapiens blood plasma plasma blood plasma 73 75 protein cleavage protein cleavage 3i full-length ADAMTS13 cleaved VWF ∼1.7-fold faster than MDTCS. w47sa5ys-1 3 7 unspecified role unspecified role bait bait regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 19:32:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys2773ala' Tue Jun 30 19:04:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys2773Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 18:24:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu225gln' Tue Jun 30 17:34:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu225Gln' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 4 2-RHS vwf-adamts13-1 3 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 14:48:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu225gln' Tue Jun 30 18:28:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu225Gln' MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 4 1-LHS vwf-adamts13-2 3 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 18:57:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu225gln' Tue Jun 30 18:39:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu225Gln' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 4 1-RHS vwf-adamts13-3 3 7 unspecified role unspecified role bait bait regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 18:16:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys2773ala' Tue Jun 30 20:20:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys2773Ala' P04275:p.Gly1505Glu mutation mutation certain certain sequence position certain certain certain sequence position certain G E Wed May 02 18:16:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1505glu' Tue Jun 30 20:00:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1505Glu' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 14:25:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu225gln' Tue Jun 30 18:41:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu225Gln' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 4 3-RHS vwf-adamts13-4 3 7 unspecified role unspecified role bait bait c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P04275:p.Gly1505Glu mutation mutation certain certain sequence position certain certain certain sequence position certain G E Wed May 02 17:21:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1505glu' Tue Jun 30 17:36:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1505Glu' regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 20:20:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys2773ala' Tue Jun 30 20:21:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys2773Ala' ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 14:24:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu225gln' Tue Jun 30 18:46:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu225Gln' MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 4 3-LHS vwf-adamts13-5 3 7 unspecified role unspecified role bait bait regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:42:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys2773ala' Tue Jun 30 16:30:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys2773Ala' mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. 5 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 15:14:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu225gln' Tue Jun 30 17:36:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu225Gln' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 4 4-RHS