IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The interaction of KIR3DL1*001 with HLA class I molecules is dependent upon molecular microarchitecture within the Bw4 epitope. The interaction of KIR3DL1*001 with HLA class I molecules is dependent upon molecular microarchitecture within the Bw4 epitope. Journal of immunology (Baltimore, Md. : 1950) 2015 imex curation Saunders PM., Vivian JP., Baschuk N., Beddoe T., Widjaja J., O'Connor GM., Hitchen C., Pymm P., Andrews DM., Gras S., McVicar DW., Rossjohn J., Brooks AG. jamie.rossjohn@monash.edu,agbrooks@unimelb.edu.au 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Saunders PM., Vivian JP., Baschuk N., Beddoe T., Widjaja J., O'Connor GM., Hitchen C., Pymm P., Andrews DM., Gras S., McVicar DW., Rossjohn J., Brooks AG. jamie.rossjohn@monash.edu,agbrooks@unimelb.edu.au Accepted 2017-MAR-31 AT 08:49 BST AT 08:49 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2015 imex curation altered The interaction of KIR3DL1*001 with HLA class I molecules is dependent upon molecular microarchitecture within the Bw4 epitope. The interaction of KIR3DL1*001 with HLA class I molecules is dependent upon molecular microarchitecture within the Bw4 epitope. Journal of immunology (Baltimore, Md. : 1950) 2015 imex curation Saunders PM., Vivian JP., Baschuk N., Beddoe T., Widjaja J., O'Connor GM., Hitchen C., Pymm P., Andrews DM., Gras S., McVicar DW., Rossjohn J., Brooks AG. jamie.rossjohn@monash.edu,agbrooks@unimelb.edu.au 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Saunders PM., Vivian JP., Baschuk N., Beddoe T., Widjaja J., O'Connor GM., Hitchen C., Pymm P., Andrews DM., Gras S., McVicar DW., Rossjohn J., Brooks AG. jamie.rossjohn@monash.edu,agbrooks@unimelb.edu.au Accepted 2017-MAR-31 AT 08:49 BST AT 08:49 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2015 imex curation altered ebna3_ebvg Epstein-Barr nuclear antigen 3 Epstein-Barr nuclear antigen 3A BLRF3-BERF1 EBNA3 protein protein ebvg Epstein-Barr virus (strain B95-8) Human herpesvirus 4 MDKDRPGDPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELSDSSSEKEAEDAQLEPAQKGTKRKRVDHDAGGSAPARPMLPPQPDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQISNTEMYIMYAMAIRQAIRDRRRNPASRRDQAKWRLQTLAAGWPMGYQAYSSWMYSYTDHQTAPTFVQLQATLGCTGGRRCHVTFSAGTFKPPRCTPGDRQWLYVQSSVGNIVQSCNPRYSIFFDYMAIHRSLTKIWEEVLTPDQRVSFMEFLGFLQRTDLSYIKSFVSDALGTTSIQTPWIDDNSSTETAQAWNAGFLRGRAYGLDLLRTEGEHVEGATGETREESEDTESDGDDEDLPCIVSRGGPKVKRPPIFIRRLHRLLLMRAGKRTEQGKEVLEKARGSTYGTPRPPVPKPRPEVPQSDETATSHGSAQVPEPPTIHLAAQGMAYPLHEQRGMAPCPVAQAPHTPLPPVSPGDQLPGVSSDGRVACAPVPAPAGPIVRPWEPSLTQAAGQAFAPVRPQHMPVEPVPVPTVALERPVYPKPVRPAPPKIAMQGPGETSGIRRARERWRPAPWTPNPPRSPSQMSVRDRLARLRAEAQVKQASVEVQPPQVTQVSPQQPMEGPGAPFSQVADVVHTPGVPAMQPQYFDLPLIQPISQGAPVAPLRASMGPVPPVPATQPQYFDIPLTEPINQGASAAHFLPQQPMEGPLVPEQWMFPGAALSQSVRPGVAQSQYFDLPLTQPITHGAPAAHFLHQPPMEGPWVPEQWMFQGAPPSQGTDVVQHQLDALGYPLHALNHPGVPVSPAVNQYHLSQAAFGLPIDEDESGEGSDTSEPCEALDLSIHGRPCPQAPEWPVQGEGGQDATEVLDLSIHGRPRPRTPEWPVQGESGQNVTDHEPRRVVVSAIVHMCQDDEFPDLQDPPDEA DABB76CCC45C2E5C 1b57_human HLA class I histocompatibility antigen, B-57 alpha chain HLA-B Bw-57 HLAB MHC class I antigen B*57 protein protein human Homo sapiens Human MRVTAPRTVLLLLWGAVALTETWAGSHSMRYFYTAMSRPGRGEPRFIAVGYVDDTQFVRFDSDAASPRMAPRAPWIEQEGPEYWDGETRNMKASAQTYRENLRIALRYYNQSEAGSHIIQVMYGCDVGPDGRLLRGHDQSAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAARVAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA The protein could not be merged with EBI-1046513 because of some conflicts with the protein sequence (features which cannot be shifted). C1EFCF089096AFF9 igkc_human Immunoglobulin kappa constant IGKC Ig kappa chain C region Ig kappa chain C region AG Ig kappa chain C region CUM Ig kappa chain C region EU Ig kappa chain C region OU Ig kappa chain C region ROY Ig kappa chain C region TI protein protein human Homo sapiens Human RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC B97BEEBBA4D0B291 1b08_human_deprecated MHC class I antigen B*8 HLA-B HLAB protein protein human Homo sapiens Human MLVMAPRTVLLLLSAALALTETWAGSHSMRYFDTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQIFKTNTQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHNQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAARVAEQDRAYLEGTCVEWLRRYLENGKDTLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA This protein is not up-to-date anymore with the uniprot protein because of feature conflicts. 9B2A9760021680DF p61769-pro_0000018779 Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM 21 119 B6EE395752B3322D ki3l1_human Killer cell immunoglobulin-like receptor 3DL1 KIR3DL1 CD158E NKAT3 NKB1 CD158 antigen-like family member E HLA-BW4-specific inhibitory NK cell receptor MHC class I NK cell receptor Natural killer-associated transcript 3 p70 natural killer cell receptor clones CL-2/CL-11 protein protein human Homo sapiens Human MSLMVVSMACVGLFLVQRAGPHMGGQDKPFLSAWPSAVVPRGGHVTLRCHYRHRFNNFMLYKEDRIHIPIFHGRIFQESFNMSPVTTAHAGNYTCRGSHPHSPTGWSAPSNPVVIMVTGNHRKPSLLAHPGPLVKSGERVILQCWSDIMFEHFFLHKEGISKDPSRLVGQIHDGVSKANFSIGPMMLALAGTYRCYGSVTHTPYQLSAPSDPLDIVVTGPYEKPSLSAQPGPKVQAGESVTLSCSSRSSYDMYHLSREGGAHERRLPAVRKVNRTFQADFPLGPATHGGTYRCFGSFRHSPYEWSDPSDPLLVSVTGNPSSSWPSPTEPSSKSGNPRHLHILIGTSVVIILFILLLFFLLHLWCSNKKNAAVMDQEPAGNRTANSEDSDEQDPEEVTYAQLDHCVFTQRKITRPSQRPKTPPTDTILYTELPNAKPRSKVVSCP 47DEA12BBAFDEC53 hla_b-kir3dl1-1 2 4 unspecified role unspecified role bait bait p.Leu106Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R Wed May 02 19:00:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu106arg' p.Arg107Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R G Wed May 02 19:27:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg107gly' p.[Ile104Asn;Leu106Arg] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I N certain certain sequence position certain certain certain sequence position certain L R Wed May 02 16:58:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104asn,leu106arg' p.[Ile104Asn;Arg107Gly] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I N certain certain sequence position certain certain certain sequence position certain R G Wed May 02 16:47:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104asn,arg107gly' extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.Ile104Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I N Tue May 09 10:44:08 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ile80asn' Wed May 02 16:17:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104asn' ecoli Escherichia coli (strain K12) 7 ancillary ancillary unspecified role unspecified role whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey possible_his_and_secretion_tags tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 ancillary ancillary unspecified role unspecified role fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 15 18 physical association physical association Table 1 B5701 allele Kd for the HLA-B5701 Ile104Asn mutant was >300uM hla_b-kir3dl1-2 2 7 ancillary ancillary unspecified role unspecified role whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait p.[Asn104Ile;Arg106_Gly107delinsLeuArg] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain N I certain certain sequence position certain certain certain sequence position certain RG LR Tue May 09 10:52:36 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'asn104ile,arg106leu,gly107arg' Wed May 02 18:55:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn104ile,arg_gly106-107leu_arg' extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain possible_his_and_secretion_tags tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 ancillary ancillary unspecified role unspecified role fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 34 29 26 physical association physical association Table 1i B0801 allele Kd for the R106L mutant was >300uM No binding was shown by the wild type. hla_b-kir3dl1-3_x14-1 1 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component p.[Asn104Ile;Arg106_Gly107delinsLeuArg] mutation mutation certain certain sequence position certain certain certain sequence position certain N I certain certain sequence position certain certain certain sequence position certain RG LR Tue May 09 10:44:15 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'asn104ile,arg106leu,gly107arg' Wed May 02 15:20:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn104ile,arg_gly106-107leu_arg' extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 2 B0801 allele 2A R work = 0.178 R free = 0.229 hla_b-kir3dl1-x11-1 1 4 unspecified role unspecified role neutral component neutral component p.[Ile104Asn;Leu106Arg;Arg107Gly] mutation mutation certain certain sequence position certain certain certain sequence position certain I N certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain R G Wed May 02 20:13:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104asn,leu106arg,arg107gly' extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 2 B5701 allele A 2.15 R work =0.19 R free =0.23 b2m-ebna3_x13-1 1 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component p.Asn104Ile mutation mutation certain certain sequence position certain certain certain sequence position certain N I Wed May 02 17:51:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn104ile' extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 2 B0801 allele 1.8A R work = 0.17 R free = 0.21 b2m-hla_b-x12-1 1 4 unspecified role unspecified role neutral component neutral component extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.Ile104Asn mutation mutation certain certain sequence position certain certain certain sequence position certain I N Tue May 09 10:35:25 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ile80asn' Wed May 02 19:25:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104asn' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 2 B5701 allele A 1.8 R work = 0.18 R free = 0.22