DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS biology 2014 imex curation Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. DIP original experiment: DIP-262229X PLoS biology 2014 IMEx The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS biology 2014 imex curation Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. DIP original experiment: DIP-262226X PLoS biology 2014 IMEx The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS biology 2014 imex curation Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. DIP original experiment: DIP-262225X PLoS biology 2014 IMEx The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS biology 2014 imex curation Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. DIP original experiment: DIP-262228X PLoS biology 2014 IMEx The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS biology 2014 imex curation Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. DIP original experiment: DIP-262230X PLoS biology 2014 IMEx The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS biology 2014 imex curation Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pietrobon V., Fréon K., Hardy J., Costes A., Iraqui I., Ochsenbein F., Lambert SA. DIP original experiment: DIP-262227X PLoS biology 2014 IMEx The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. pcna_schpo Proliferating cell nuclear antigen pcn1 pcn SPBC16D10.09 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLEARFQQAALLKKLLDAIKELVTDANFDCNDNGISLQAMDSSHVALVSMLIKSDGFEPYRCDRNIALGINLNALSKVLRCAQNEDLVTLKAEDTPEVLNLVFESEKNDRISDYDVKLMDIDQEHLGIPDIEYDATITMPAAEFQRITRDLLTLSDSVTINASKEGVRFSCKGDIGNGSTTLKQHTDLSDQDQSIEISLTQAVTLTFSLKYLAQFTKATPLATRVTLSMSNDVPLLVEYKMESGFLRFYLAPKIGEEDEE 1EE5B2A27A899C4E 1EE5B2A27A899C4E rlf2_schpo Chromatin assembly factor 1 subunit rlf2 rlf2 SPBC29A10.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNSESVDSDVAASTSNKGNELCSSSTDITSLSVSSPNESVIHSSHSASEADEYVCKLSYEGNRKKRIYNGSAEAGKEKKLQKQRAQEERIRQKEAERLKREKERQQREQEKKLREQEKIAAKKMKELEKLEKERIRLQEQQRRKEERDQKLREKEEAQRLRQEQILNKERQQLKLNNFFTKGVEKRIAPNENFVADKTDELNEFEKEFRPFFIKHQMSLSKYPSPNESDSFLDEVLSTSKSYPLKLNDIFTPSDAVSSANSLGVSNRNSENEVRQLMSAYQDPSVSKPQEILSCLSQIPIKFIFFYQDVRPPYFGSYTKTHSHGSNVLLNPWLEDEDIDYTYDSEAEWVADEEDDGEDLESEDEEVDNSDDIVEDGDNAFVDDEDDDKDSVNASNTHRSSGPLEVIVEGPVWDSKFLPDFNCLSLIEPISSFSASTYLQIDPKEDLWASQDTAPASSGMTIGPTSSLSDDLQVRFPSEDIPKFIEYVRNSHDNKVFLIENLRHMFPYVTKNIISETLGKVAVRKGKSVSDGWIIKENFASLLSS B7413523836A9C4A B7413523836A9C4A hus2_schpo ATP-dependent DNA helicase hus2/rqh1 rqh1 hus2 rad12 rec9 SPAC2G11.12 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTVTKTNLNRHLDWFFRESPQKIENVTSPIKTLDFVKVKVSSSDIVVKDSIPHKSKNVFDDFDDGYAIDLTEEHQSSSLNNLKWKDVEGPNILKPIKKIAVPASESEEDFDDVDEEMLRAAEMEVFQSCQPLAVNTADTTVSHSTSSSNVPRSLNKIHDPSRFIKDNDVENRIHVSSASKVASISNTSKPNPIVSENPISATSVSIEIPIKPKELSNNLPFPRLNNNNTNNNNDNNAIEKRDSASPTPSSVSSQISIDFSTWPHQNLLQYLDILRDEKSEISDRIIEVMERYPFSSRFKEWIPKRDILSQKISSVLEVLSNNNNSNNNNGNNGTVPNAKTFFTPPSSITQQVPFPSTIIPESTVKENSTRPYVNSHLVANDKITATPFHSEAVVSPLQSNIRNSDIAEFDEFDIDDADFTFNTTDPINDESGASSDVVVIDDEEDDIENRPLNQALKASKAAVSNASLLQSSSLDRPLLGEMKDKNHKVLMPSLDDPMLSYPWSKEVLGCLKHKFHLKGFRKNQLEAINGTLSGKDVFILMPTGGGKSLCYQLPAVIEGGASRGVTLVISPLLSLMQDQLDHLRKLNIPSLPLSGEQPADERRQVISFLMAKNVLVKLLYVTPEGLASNGAITRVLKSLYERKLLARIVIDEAHCVSHWGHDFRPDYKQLGLLRDRYQGIPFMALTATANEIVKKDIINTLRMENCLELKSSFNRPNLFYEIKPKKDLYTELYRFISNGHLHESGIIYCLSRTSCEQVAAKLRNDYGLKAWHYHAGLEKVERQRIQNEWQSGSYKIIVATIAFGMGVDKGDVRFVIHHSFPKSLEGYYQETGRAGRDGKPAHCIMFYSYKDHVTFQKLIMSGDGDAETKERQRQMLRQVIQFCENKTDCRRKQVLAYFGENFDKVHCRKGCDICCEEATYIKQDMTEFSLQAIKLLKSISGKATLLQLMDIFRGSKSAKIVENGWDRLEGAGVGKLLNRGDSERLFHHLVSEGVFVEKVEANRRGFVSAYVVPGRQTIINSVLAGKRRIILDVKESSSKPDTSSRSLSRSKTLPALREYQLKSTTASVDCSIGTREVDEIYDSQMPPVKPSLIHSRNKIDLEELSGQKFMSEYEIDVMTRCLKDLKLLRSNLMAIDDSRVSSYFTDSVLLSMAKKLPRNVKELKEIHGVSNEKAVNLGPKFLQVIQKFIDEKEQNLEGTELDPSLQSLDTDYPIDTNALSLDHEQGFSDDSDSVYEPSSPIEEGDEEVDGQRKDILNFMNSQSLTQTGSVPKRKSTSYTRPSKSYRHKRGSTSYSRKRKYSTSQKDSRKTSKSANTSFIHPMVKQNYR 13A9A5B94E5A9BD1 yeg3_schpo Uncharacterized WD repeat-containing protein C26H5.03 SPAC26H5.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MRAEVLQIRWHYDANDDHTPIYSVDFQKNSLNKFATCGGDSKIRIWQLITSESSTKVEYLSTLSRHTQAVNVVRFNPEGNILATAGDEGTIMLWVPTNTPITTLADDAEELALAKEYWKVKIVCRSMGSEIYDLCWSVDSNFLIAGAMDNSLRLYDAHTGQLLTQKFDHSHYVQGVCWDPLNQYIVSESSDRSICLYEIQEEKKNPKKFQLVLKSRICRIEYNVTKFELISVTKPLNNDESSGISEPIETSNNNESPVSKHEALSSTANIVKDGSLERTEPPNSLNSKISYSLYCNETLVSFFRRPAFSPDGLLLVTPAGRLRPHGQPNFEVPYTAYIYTRGSITKQPVACLNGFKKPVIAVRFSPIHYELNSFSNFSFTSVSFNLPYRMVFAVACQDAVYIYDTQTCKPFYRAVNLHYSNLTDIAWNDDGNVLLMTSIDGFCSVITFEPGELGVKSQHKISLPEKRSASPSSIDDSQDNTAGGPATTTLIPRKVESSKVSKKRIAPTPVYP 6010733FED6CC87C pcf1-pcna-1 3 8 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 9 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast association association pcf1-pcf2-1 1 8 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 10 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast association association pcf1-pcna-2 6 8 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 9 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast association association pcf1-pcna-3 2 8 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q1MTN9:p.[Gln172Ala;Leu175Ala;Phe178Ala;Phe179Ala] Q172A/L175A/F178A/F179A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q172A/L175A/F178A/F179A'. Wed May 02 17:21:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln172ala,leu175ala,phe178ala,phe179ala' Tue Jun 30 15:50:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln172Ala;Leu175Ala;Phe178Ala;Phe179Ala]' schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association pcf2-pcna-1 4 10 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association pcf1-pcf2-2 5 8 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 10 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association